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The map label for this gene is potI [H]

Identifier: 86748581

GI number: 86748581

Start: 1644717

End: 1645532

Strand: Direct

Name: potI [H]

Synonym: RPB_1456

Alternate gene names: 86748581

Gene position: 1644717-1645532 (Clockwise)

Preceding gene: 86748580

Following gene: 86748583

Centisome position: 30.85

GC content: 68.14

Gene sequence:

>816_bases
ATGGCCGCGCGCGCCGCCCGCCTGTCCCGCTTCAACCTCGTGTCGCTGACGCTGGGACTTGCGTTCCTGTATCTGCCGAT
CGTCATCATGGTGATCTACTCGTTCAACGATTCGCGCCTGGTCACGGTGTGGGGCGGCTGGTCGCTGCGCTGGTATCGCG
AATTGTTGCAGGACGAGGCGATGCTGGCGGCGGCGTGGATGAGCCTGAAGGTGGCGCTGGCGTCGGCGACCGCGGCGGCG
GTGTTCGGCACGCTGGCGGCGGTGGCGCTGTCGCGCGCCGGGCACTTTCGCGGCTGGACGTTGTTCTCCGGGATGCTGTA
TGCGCCGCTGGTGATGCCGGAAGTGATCACCGGCCTGTCGCTGCTGCTGCTGTTCGTGGCGCTGAATGCCGAGCGCGGCT
TCTGGACGGTAACGATCGCGCATACCACGCTGACGATGTGCTTCGTCGCGGTGGTGGTGCAGTCGCGCCTGCACGCGCTC
GACCGCAGCGTCGAGGAGGCGGCGATGGATCTCGGCTGCGGCCCGGTCGAGGCGTTCTTGCAGGCCACGGTGCCGCTGAT
CTGGCCGGCGATCGCGGCCGGCTGGATGCTGGCCTTCGCGCTGTCGCTCGACGATCTGGTGATCGCCAGCTTCACCACCG
GCCCGGGCTCGGCGACGTTGCCGATCCGGATCTATTCGGAGATCAGGCTCGGCGTGAAGCCGCAGATCAACGCGGTCTGC
ACCCTGATCATCGCGGTCGTCGCGCTGGCGATCGTGCTCGCCTCGCTGGCGTCGAAACTGTCGCGCGCCAAGGCCGACAG
CGCCGCGCCGATGTGA

Upstream 100 bases:

>100_bases
CGGTGGCCTCGGCGATCGCGGTGGTGCTGCTCGGCGTGCTGTTGCTGCCGCTCCTGATCTACGAGCGGCTGCAGCGGCGG
CAGCTCGAAGGAGAGCGGTG

Downstream 100 bases:

>100_bases
GGCGCGATGTGACGCGCCTGCTCTCCGTCATAGCGAGAAGGCGAAGCCGACGAAGCAATCCAGAGGCGCCATGCACGGAG
CGCTGGATTGCTTCGCTGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAARAARLSRFNLVSLTLGLAFLYLPIVIMVIYSFNDSRLVTVWGGWSLRWYRELLQDEAMLAAAWMSLKVALASATAAA
VFGTLAAVALSRAGHFRGWTLFSGMLYAPLVMPEVITGLSLLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLHAL
DRSVEEAAMDLGCGPVEAFLQATVPLIWPAIAAGWMLAFALSLDDLVIASFTTGPGSATLPIRIYSEIRLGVKPQINAVC
TLIIAVVALAIVLASLASKLSRAKADSAAPM

Sequences:

>Translated_271_residues
MAARAARLSRFNLVSLTLGLAFLYLPIVIMVIYSFNDSRLVTVWGGWSLRWYRELLQDEAMLAAAWMSLKVALASATAAA
VFGTLAAVALSRAGHFRGWTLFSGMLYAPLVMPEVITGLSLLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLHAL
DRSVEEAAMDLGCGPVEAFLQATVPLIWPAIAAGWMLAFALSLDDLVIASFTTGPGSATLPIRIYSEIRLGVKPQINAVC
TLIIAVVALAIVLASLASKLSRAKADSAAPM
>Mature_270_residues
AARAARLSRFNLVSLTLGLAFLYLPIVIMVIYSFNDSRLVTVWGGWSLRWYRELLQDEAMLAAAWMSLKVALASATAAAV
FGTLAAVALSRAGHFRGWTLFSGMLYAPLVMPEVITGLSLLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLHALD
RSVEEAAMDLGCGPVEAFLQATVPLIWPAIAAGWMLAFALSLDDLVIASFTTGPGSATLPIRIYSEIRLGVKPQINAVCT
LIIAVVALAIVLASLASKLSRAKADSAAPM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=247, Percent_Identity=54.251012145749, Blast_Score=248, Evalue=2e-67,
Organism=Escherichia coli, GI1787368, Length=237, Percent_Identity=40.9282700421941, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787714, Length=237, Percent_Identity=37.1308016877637, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29014; Mature: 28883

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAARLSRFNLVSLTLGLAFLYLPIVIMVIYSFNDSRLVTVWGGWSLRWYRELLQDEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
MLAAAWMSLKVALASATAAAVFGTLAAVALSRAGHFRGWTLFSGMLYAPLVMPEVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLHALDRSVEEAAMDLGCGPVEAFL
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QATVPLIWPAIAAGWMLAFALSLDDLVIASFTTGPGSATLPIRIYSEIRLGVKPQINAVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEHHHCCCCCCHHHHH
TLIIAVVALAIVLASLASKLSRAKADSAAPM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AARAARLSRFNLVSLTLGLAFLYLPIVIMVIYSFNDSRLVTVWGGWSLRWYRELLQDEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
MLAAAWMSLKVALASATAAAVFGTLAAVALSRAGHFRGWTLFSGMLYAPLVMPEVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLHALDRSVEEAAMDLGCGPVEAFL
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QATVPLIWPAIAAGWMLAFALSLDDLVIASFTTGPGSATLPIRIYSEIRLGVKPQINAVC
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEEHHHCCCCCCHHHHH
TLIIAVVALAIVLASLASKLSRAKADSAAPM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]