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The map label for this gene is pyk [H]

Identifier: 86748548

GI number: 86748548

Start: 1610469

End: 1611902

Strand: Reverse

Name: pyk [H]

Synonym: RPB_1423

Alternate gene names: 86748548

Gene position: 1611902-1610469 (Counterclockwise)

Preceding gene: 86748549

Following gene: 86748542

Centisome position: 30.23

GC content: 67.29

Gene sequence:

>1434_bases
ATGAGACGTCTGCGCCGGATCAAGATCTTCGCGACACTCGGCCCCGTGTCTTCAGACAGCGCGATGATCCGCAAGCTGTT
CGAGGCGGGCGCCGATGTGTTTCGGATCAACATGAGCCACACCTCGCACGACAAGATGCGCGAGCTGATCAAGACCATCC
GCAATGTCGAATCGAGCTACGGCCGGCCGATCGGCATTCTGGTCGATCTGCAGGGACCGAAGCTGCGGGTCGGCATGTTC
GGCAATGGGCCGATCCAGCTCAACAACGGCGCCACCTTCGTGCTGGATTCCAGCAAGGAGCCCGGCGACGCCGATCGCGT
CCACCTGCCGCATCCGGAAATTCTCTCGGCGCTGAAGGTCGGCGACGCGATGCTGCTCGACGACGGCAAGGTCCGGCTGA
TCTGCGAAGAAACCTCACCGGATCGCGCCGTCACCCGCGTGGTGATCGGCGGCAAGATGAGCGACCGCAAGGGCGTCAGC
CTGCCCGACACCGATCTGCCGATGTCGGCGATGACCAACAAGGACCGCGCCGACCTCGAGGCGGCCTGCGAGGCCGGCAT
CGACTGGGTGGCGCTGTCCTTCGTGCAGCGCGCCGAGGATGTCGCCGAAGCCAAGCGGATGATCCGCGGGCGCGCGGCGG
TGATGGCGAAGATCGAGAAGCCGCAGGCGATCGACCGGCTCGACGAGATCCTCGGCGTCTCCGACGCGCTGATGGTGGCG
CGCGGCGACCTCGGCGTCGAGATGCCGCTGGAGCGCGTGCCAAGTCTGCAAAAGCAGATGACGCGGATGGCGCGCCGCGC
CGGCAAGCCGGTGGTGATCGCGACCCAGATGCTGGAATCGATGATCTCCAGCCCGGTGCCGACCCGCGCCGAAGTCTCCG
ACGTCGCCACCGCGGTCTATGAGGGCGCCGACGCGATCATGTTGTCGGCGGAATCCGCCGCTGGCAAATATCCGGTCGAA
GCCGTCTCGACGATGAACCGGATCGGCGAGGAGGTCGAACGCGATACGATCTACCGCACGGTGGTGACGGCGCAGCGGCC
TGAGCCCGAAGCAACCGCCGGCGACGCGATCGCCGGCGCGGCGCGGCAGGTCGCCGAAACGCTCGACCTGTCGGCGATCA
TCTGCTGGACCAGTTCGGGCTCGACCGCGCTGCGCGTCGCGCGCGAGCGGCCGAAAGTGCCGGTGGTGGCGATCACGCCG
AACGTCAACACCGGGCGCAAGCTGTCGGCGGTGTGGGGCGTGCATTGCGTCGTGGCGGAAGATGCCAAGGATCAGGACGA
CATGGTCGACCGCGCCGGCCGCATCGCGTTCCGCGACGGCTTCGCCAAATCCGGCCAGCGCGTCATCATCGTCGCCGGCG
TCCCGCTCGGCACCCCCGGCGCCACCAACATGGTGCGGATCGCCTATGTGGGGCCGACCGATACGGATGTGTGA

Upstream 100 bases:

>100_bases
GCCCGCCGCCGGGCGCGGGCATCCCCGGCATGTCGCCGACGCCGGGCCGCACCGCGCCGGCCACGCCCACGGCACCACCC
GGTGACGCCGGGACCAAGCC

Downstream 100 bases:

>100_bases
GGGCCGCAGCCCGATTCAGGCTGCTTCCTCGATCCCGACCACCAGGCGCTTGCCCAGCGCACGTAGCGCCTGGACCATCG
CGGGTAGCTTGGTCGGGTGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRLRRIKIFATLGPVSSDSAMIRKLFEAGADVFRINMSHTSHDKMRELIKTIRNVESSYGRPIGILVDLQGPKLRVGMF
GNGPIQLNNGATFVLDSSKEPGDADRVHLPHPEILSALKVGDAMLLDDGKVRLICEETSPDRAVTRVVIGGKMSDRKGVS
LPDTDLPMSAMTNKDRADLEAACEAGIDWVALSFVQRAEDVAEAKRMIRGRAAVMAKIEKPQAIDRLDEILGVSDALMVA
RGDLGVEMPLERVPSLQKQMTRMARRAGKPVVIATQMLESMISSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKYPVE
AVSTMNRIGEEVERDTIYRTVVTAQRPEPEATAGDAIAGAARQVAETLDLSAIICWTSSGSTALRVARERPKVPVVAITP
NVNTGRKLSAVWGVHCVVAEDAKDQDDMVDRAGRIAFRDGFAKSGQRVIIVAGVPLGTPGATNMVRIAYVGPTDTDV

Sequences:

>Translated_477_residues
MRRLRRIKIFATLGPVSSDSAMIRKLFEAGADVFRINMSHTSHDKMRELIKTIRNVESSYGRPIGILVDLQGPKLRVGMF
GNGPIQLNNGATFVLDSSKEPGDADRVHLPHPEILSALKVGDAMLLDDGKVRLICEETSPDRAVTRVVIGGKMSDRKGVS
LPDTDLPMSAMTNKDRADLEAACEAGIDWVALSFVQRAEDVAEAKRMIRGRAAVMAKIEKPQAIDRLDEILGVSDALMVA
RGDLGVEMPLERVPSLQKQMTRMARRAGKPVVIATQMLESMISSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKYPVE
AVSTMNRIGEEVERDTIYRTVVTAQRPEPEATAGDAIAGAARQVAETLDLSAIICWTSSGSTALRVARERPKVPVVAITP
NVNTGRKLSAVWGVHCVVAEDAKDQDDMVDRAGRIAFRDGFAKSGQRVIIVAGVPLGTPGATNMVRIAYVGPTDTDV
>Mature_477_residues
MRRLRRIKIFATLGPVSSDSAMIRKLFEAGADVFRINMSHTSHDKMRELIKTIRNVESSYGRPIGILVDLQGPKLRVGMF
GNGPIQLNNGATFVLDSSKEPGDADRVHLPHPEILSALKVGDAMLLDDGKVRLICEETSPDRAVTRVVIGGKMSDRKGVS
LPDTDLPMSAMTNKDRADLEAACEAGIDWVALSFVQRAEDVAEAKRMIRGRAAVMAKIEKPQAIDRLDEILGVSDALMVA
RGDLGVEMPLERVPSLQKQMTRMARRAGKPVVIATQMLESMISSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKYPVE
AVSTMNRIGEEVERDTIYRTVVTAQRPEPEATAGDAIAGAARQVAETLDLSAIICWTSSGSTALRVARERPKVPVVAITP
NVNTGRKLSAVWGVHCVVAEDAKDQDDMVDRAGRIAFRDGFAKSGQRVIIVAGVPLGTPGATNMVRIAYVGPTDTDV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=37.037037037037, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=37.037037037037, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=37.2708757637475, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=37.525354969574, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=37.525354969574, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=35.191637630662, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=35.191637630662, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=30.379746835443, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=30.379746835443, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.3738317757009, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=38.7966804979253, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1787965, Length=463, Percent_Identity=35.85313174946, Blast_Score=289, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=34.297520661157, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=483, Percent_Identity=36.8530020703934, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=483, Percent_Identity=36.8530020703934, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506829, Length=483, Percent_Identity=36.8530020703934, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=483, Percent_Identity=36.8530020703934, Blast_Score=268, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=34.020618556701, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=32.7902240325866, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=35.1020408163265, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=35.1020408163265, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=30.7216494845361, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=33.8235294117647, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24646914, Length=240, Percent_Identity=36.25, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51361; Mature: 51361

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLRRIKIFATLGPVSSDSAMIRKLFEAGADVFRINMSHTSHDKMRELIKTIRNVESSY
CCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GRPIGILVDLQGPKLRVGMFGNGPIQLNNGATFVLDSSKEPGDADRVHLPHPEILSALKV
CCCEEEEEECCCCEEEEEEECCCCEEECCCEEEEECCCCCCCCCCCEECCCHHHHHHHHC
GDAMLLDDGKVRLICEETSPDRAVTRVVIGGKMSDRKGVSLPDTDLPMSAMTNKDRADLE
CCEEEECCCCEEEEECCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHHH
AACEAGIDWVALSFVQRAEDVAEAKRMIRGRAAVMAKIEKPQAIDRLDEILGVSDALMVA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEE
RGDLGVEMPLERVPSLQKQMTRMARRAGKPVVIATQMLESMISSPVPTRAEVSDVATAVY
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHH
EGADAIMLSAESAAGKYPVEAVSTMNRIGEEVERDTIYRTVVTAQRPEPEATAGDAIAGA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ARQVAETLDLSAIICWTSSGSTALRVARERPKVPVVAITPNVNTGRKLSAVWGVHCVVAE
HHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEHHHCEEEEEEC
DAKDQDDMVDRAGRIAFRDGFAKSGQRVIIVAGVPLGTPGATNMVRIAYVGPTDTDV
CCCCHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MRRLRRIKIFATLGPVSSDSAMIRKLFEAGADVFRINMSHTSHDKMRELIKTIRNVESSY
CCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GRPIGILVDLQGPKLRVGMFGNGPIQLNNGATFVLDSSKEPGDADRVHLPHPEILSALKV
CCCEEEEEECCCCEEEEEEECCCCEEECCCEEEEECCCCCCCCCCCEECCCHHHHHHHHC
GDAMLLDDGKVRLICEETSPDRAVTRVVIGGKMSDRKGVSLPDTDLPMSAMTNKDRADLE
CCEEEECCCCEEEEECCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHHH
AACEAGIDWVALSFVQRAEDVAEAKRMIRGRAAVMAKIEKPQAIDRLDEILGVSDALMVA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEE
RGDLGVEMPLERVPSLQKQMTRMARRAGKPVVIATQMLESMISSPVPTRAEVSDVATAVY
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHH
EGADAIMLSAESAAGKYPVEAVSTMNRIGEEVERDTIYRTVVTAQRPEPEATAGDAIAGA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ARQVAETLDLSAIICWTSSGSTALRVARERPKVPVVAITPNVNTGRKLSAVWGVHCVVAE
HHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEHHHCEEEEEEC
DAKDQDDMVDRAGRIAFRDGFAKSGQRVIIVAGVPLGTPGATNMVRIAYVGPTDTDV
CCCCHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA