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The map label for this gene is rpoE [H]

Identifier: 86748511

GI number: 86748511

Start: 1578460

End: 1579005

Strand: Reverse

Name: rpoE [H]

Synonym: RPB_1386

Alternate gene names: 86748511

Gene position: 1579005-1578460 (Counterclockwise)

Preceding gene: 86748512

Following gene: 86748507

Centisome position: 29.62

GC content: 62.45

Gene sequence:

>546_bases
ATGCCTCTCACCGATTCTCTTCGAGACGACATTCTGGCGGCAGTGCCGAGCTTGCGTGCGTTCGCGATTTCGCTCAGCGG
AAACGGTGATCGCGCGGACGATCTCGTGCAGGAAACCCTGCTTCGCGCGCTGGCCAATATCGACTCATTCCAGCCGGGCT
CGAATTTGCCGGCCTGGCTGTTCACGATTCTGCGCAATCTGTTCCGGTCCGACTATCGGAAGCGGCGGCGCGAGGTCGAG
GACGCTGATGGCAGTTATGCCAAGACGCTGAAATCGCAGCCGGGCCAGAACGCGCATCTGGAGTTCGAAGAATTTCGAGC
GGCGCTGGAAAAGCTTCCGCAGGATCAGCGCGAAGCGTTGATCCTGGTCGGTGCCTCGGGCTTCTCCTATGAAGACGCCG
CCGCCATCTGCGGCTGCGCCGTGGGCACCATCAAGAGCCGCGTCAACCGCGCGCGGTCCAAGCTGAGTGCGTTGCTCTAT
GTCGACGGCGCCGAGGATTTCGGACCCGACGACACAGTGCGCGCCGTGATCGGCAGCAACGGCTGA

Upstream 100 bases:

>100_bases
GGCAAGGCGTACCGGATCGGTTCGCCGATCTGATCAAACGGCTGGACGACCCGCAGCAAGGCGGTCCGACAGCGGCGACC
GACAGCGAGAGGAGGGACTA

Downstream 100 bases:

>100_bases
CCAGCTTCGTCTGCAACGCCCAGCGCGACACGACCAAAATACGACCCAAGATACGATCCAGACACGCAATGCGAAGTTGG
CCCTCGGGCCTCTTCGTTAT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MPLTDSLRDDILAAVPSLRAFAISLSGNGDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVE
DADGSYAKTLKSQPGQNAHLEFEEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLSALLY
VDGAEDFGPDDTVRAVIGSNG

Sequences:

>Translated_181_residues
MPLTDSLRDDILAAVPSLRAFAISLSGNGDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVE
DADGSYAKTLKSQPGQNAHLEFEEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLSALLY
VDGAEDFGPDDTVRAVIGSNG
>Mature_180_residues
PLTDSLRDDILAAVPSLRAFAISLSGNGDRADDLVQETLLRALANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVED
ADGSYAKTLKSQPGQNAHLEFEEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLSALLYV
DGAEDFGPDDTVRAVIGSNG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=135, Percent_Identity=34.8148148148148, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19733; Mature: 19602

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDSLRDDILAAVPSLRAFAISLSGNGDRADDLVQETLLRALANIDSFQPGSNLPAWL
CCCCHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
FTILRNLFRSDYRKRRREVEDADGSYAKTLKSQPGQNAHLEFEEFRAALEKLPQDQREAL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
ILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLSALLYVDGAEDFGPDDTVRAVIGSN
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHCCCC
G
C
>Mature Secondary Structure 
PLTDSLRDDILAAVPSLRAFAISLSGNGDRADDLVQETLLRALANIDSFQPGSNLPAWL
CCCHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
FTILRNLFRSDYRKRRREVEDADGSYAKTLKSQPGQNAHLEFEEFRAALEKLPQDQREAL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
ILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLSALLYVDGAEDFGPDDTVRAVIGSN
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]