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The map label for this gene is nuoL [H]

Identifier: 86748482

GI number: 86748482

Start: 1545272

End: 1547338

Strand: Direct

Name: nuoL [H]

Synonym: RPB_1357

Alternate gene names: 86748482

Gene position: 1545272-1547338 (Clockwise)

Preceding gene: 86748481

Following gene: 86748483

Centisome position: 28.98

GC content: 69.62

Gene sequence:

>2067_bases
ATGGCCTCGCTGCTCCCCTTCGTTCCGGTCCCGGCGCTGATCGGCTTCGTGGTGCTGTCGCTGATCCCGCTGGCGCTGTC
GCGCGCGGTGGTGATCGCGATCGGCGTCGGCGCCGCGGTGCTGCCGGCAATCCTGTTCGCGCCGGTCGCGCTGTCCTGCC
TCGACGGCGCCTGCGTCGCCGGCGCGGTGGTGCCGATCCTGACGATCGACGCCGGCATGTTCGTCGCCCAGATCGCGATG
TCGCTCGATCCGCTCAGCATGGTCACCGGCGGCACGGTCAGCGTGGTGGCCGCCTTGGTGCTGATCTATTCCGGCGCGTA
TATGGCCTCCGAGCCGTTGCCGGATCTGCGCCGCTATTTCGCGCTGATGAATCTGTTTCTCGCCGGCATGCTGGCGGTGG
TGTTCGCCGCCGACGTCATCCTGCTGTTCCTCGGCTGGGAGACCATCGGCCTGTGCAGCTTCATGCTGATCGCGTTCTAC
GACAAGCTGCCGAAGGCGGTGTCGGCCGGGCGCAAGGCGCTGATCACCACCCGGATCGCCGACAGCCTGCTGCTCGCCGG
CCTGATGATGCTGTTCCTGTCCGCCGGCACCACGCGGCTCGACGGCATGCTGGCCGTGGTGCCCGGAATGGACCCCTGGC
GGCTGGCGCCGATTGCCGGCCTGATCGCGCTCGGCGCGCTTGGCAAATCGGCGCAATTTCCGTTTCACACCTGGTTGCCG
AGCGCGATGGCCGGCCCGACGCCGGTGTCGGCGCTGCTGCATTCCGCCACGATGGTGGCCGCCGGCGTGATCCTGCTGGC
GCGGCTCGCGCCGCTGTTCGCCGCCGCACCGGAAATCTCCGCGATGGTGGCGATGCTGGGCATCCTCACCGCGGCCGGCG
GCGCGCTGATCGCGGTGATCCAGACCGATATCAAGAAGCTGCTGGCGTTCTCGACCATGAGTCAGATCGGCTTCATGGTG
CTGGCGCTCGGGCTCGGCGCGCCGGCGGTGGCGCTGGCGCACTTCGCGATCCATGCCGCGTTCAAGTCGCTGCTGTTCCT
CAGCGCCGGCGTGATGAGCCACGCCTCCGGCGGCTCGACCGCGATCGAGGCGTTGCGCGGCGCGCGCTATCGCCAGCCCT
GGGCGTTCTGGAGCTTTGCGATCGGCGCCGCCTCGCTCGCCGGCCTGCCGGTGATCACGGCGGGCTGGTATTCGAAGGAA
GCGGTGCTCGCCGCGGTGTGGAGCAGCGGGCCGTGGGGCGTGCCGCTGTGGCTGCTGGCTGCCGCGGCCGCGATGCTGAC
CGCCGCCTATGCGTTCCGTGTGGTGTTCGTCGCCGGCAGCCAGTCCGCCGATCTCGCCGCGCCGCCCTGGGGCGGGGCCG
CGGTGATGATCCCGCTGGTCGTGCTCACGGCGGTTGCCGTGGTCGGTGGGCTGTCGGTGGGTGCGTTGATCGGCTTCGCC
GGTGGCACGGAGGACCATCTCCCGCTGCTGCCGACGCTGCTCGGCGCCGCCGCGCCGCTGCTCGGCGTGGTGCTGGCGCG
GCAGCTCGTCCGGCATCCGCGGGAGTTGGAGCATTTCGCGAAGCGGCTGCGCCATGTCCGCGCCCTCCGCATCGAGATCC
TGTATTACTACTACATCGTCCGGCGCTTCCGGCATCTCGCCACGGTGCTCGGTAACGCCGAAGGCACCGAAGCGGTGGCG
CAGGCGGCGGCGCTGCGCCGGGAAGGCGTTGACGCGCCGGGACTGATGGCGGACGATCCGCTCGGTCGCGCGTTCGGGAA
AGAGGCGGCCCAGCCTGATTTCATATCGCGTGCGACCGACGGGCAGACCCCTGTGGTGCGTCTCGTCGCCGGAGATCCGG
TCGGCCGGATTCTGATCATCGTCGCGCGGGTATTTGTCGGCAGTTTCGTCGCACGGTTCAATCCCGATCAGATCGATCGG
GCGTGGATGGGATTCGCAGGCGGCTTTAGTCACGCCTGGGCGCAGGCACGGCGGCTGCAGACCGGCCGCGTCCGCGATCA
TTCGCTCTGGATCGCGCTCGGCTGCGCGGCGCTTCTACTTTATGCTTGGGGGACGTCATGGCGCTGA

Upstream 100 bases:

>100_bases
CCGCAGCCGAGGTCGCGGTCGGGCTGGCGCTGTTGCTGCGCATCCGCCGCACCGCCGGCAGCGACGACAGCGACGTCATC
AGCGGCATGAAGGGATGAGC

Downstream 100 bases:

>100_bases
TCGCGCTCATTCTGCTGCCGGCCATCGGCGGCTTCGTCGCATTCTTCATCGCGGGGCGCGGTGAGCGCGAGCGCTGGGTC
ACGATCGCGACCTTCGCGCT

Product: proton-translocating NADH-quinone oxidoreductase, chain L

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MASLLPFVPVPALIGFVVLSLIPLALSRAVVIAIGVGAAVLPAILFAPVALSCLDGACVAGAVVPILTIDAGMFVAQIAM
SLDPLSMVTGGTVSVVAALVLIYSGAYMASEPLPDLRRYFALMNLFLAGMLAVVFAADVILLFLGWETIGLCSFMLIAFY
DKLPKAVSAGRKALITTRIADSLLLAGLMMLFLSAGTTRLDGMLAVVPGMDPWRLAPIAGLIALGALGKSAQFPFHTWLP
SAMAGPTPVSALLHSATMVAAGVILLARLAPLFAAAPEISAMVAMLGILTAAGGALIAVIQTDIKKLLAFSTMSQIGFMV
LALGLGAPAVALAHFAIHAAFKSLLFLSAGVMSHASGGSTAIEALRGARYRQPWAFWSFAIGAASLAGLPVITAGWYSKE
AVLAAVWSSGPWGVPLWLLAAAAAMLTAAYAFRVVFVAGSQSADLAAPPWGGAAVMIPLVVLTAVAVVGGLSVGALIGFA
GGTEDHLPLLPTLLGAAAPLLGVVLARQLVRHPRELEHFAKRLRHVRALRIEILYYYYIVRRFRHLATVLGNAEGTEAVA
QAAALRREGVDAPGLMADDPLGRAFGKEAAQPDFISRATDGQTPVVRLVAGDPVGRILIIVARVFVGSFVARFNPDQIDR
AWMGFAGGFSHAWAQARRLQTGRVRDHSLWIALGCAALLLYAWGTSWR

Sequences:

>Translated_688_residues
MASLLPFVPVPALIGFVVLSLIPLALSRAVVIAIGVGAAVLPAILFAPVALSCLDGACVAGAVVPILTIDAGMFVAQIAM
SLDPLSMVTGGTVSVVAALVLIYSGAYMASEPLPDLRRYFALMNLFLAGMLAVVFAADVILLFLGWETIGLCSFMLIAFY
DKLPKAVSAGRKALITTRIADSLLLAGLMMLFLSAGTTRLDGMLAVVPGMDPWRLAPIAGLIALGALGKSAQFPFHTWLP
SAMAGPTPVSALLHSATMVAAGVILLARLAPLFAAAPEISAMVAMLGILTAAGGALIAVIQTDIKKLLAFSTMSQIGFMV
LALGLGAPAVALAHFAIHAAFKSLLFLSAGVMSHASGGSTAIEALRGARYRQPWAFWSFAIGAASLAGLPVITAGWYSKE
AVLAAVWSSGPWGVPLWLLAAAAAMLTAAYAFRVVFVAGSQSADLAAPPWGGAAVMIPLVVLTAVAVVGGLSVGALIGFA
GGTEDHLPLLPTLLGAAAPLLGVVLARQLVRHPRELEHFAKRLRHVRALRIEILYYYYIVRRFRHLATVLGNAEGTEAVA
QAAALRREGVDAPGLMADDPLGRAFGKEAAQPDFISRATDGQTPVVRLVAGDPVGRILIIVARVFVGSFVARFNPDQIDR
AWMGFAGGFSHAWAQARRLQTGRVRDHSLWIALGCAALLLYAWGTSWR
>Mature_687_residues
ASLLPFVPVPALIGFVVLSLIPLALSRAVVIAIGVGAAVLPAILFAPVALSCLDGACVAGAVVPILTIDAGMFVAQIAMS
LDPLSMVTGGTVSVVAALVLIYSGAYMASEPLPDLRRYFALMNLFLAGMLAVVFAADVILLFLGWETIGLCSFMLIAFYD
KLPKAVSAGRKALITTRIADSLLLAGLMMLFLSAGTTRLDGMLAVVPGMDPWRLAPIAGLIALGALGKSAQFPFHTWLPS
AMAGPTPVSALLHSATMVAAGVILLARLAPLFAAAPEISAMVAMLGILTAAGGALIAVIQTDIKKLLAFSTMSQIGFMVL
ALGLGAPAVALAHFAIHAAFKSLLFLSAGVMSHASGGSTAIEALRGARYRQPWAFWSFAIGAASLAGLPVITAGWYSKEA
VLAAVWSSGPWGVPLWLLAAAAAMLTAAYAFRVVFVAGSQSADLAAPPWGGAAVMIPLVVLTAVAVVGGLSVGALIGFAG
GTEDHLPLLPTLLGAAAPLLGVVLARQLVRHPRELEHFAKRLRHVRALRIEILYYYYIVRRFRHLATVLGNAEGTEAVAQ
AAALRREGVDAPGLMADDPLGRAFGKEAAQPDFISRATDGQTPVVRLVAGDPVGRILIIVARVFVGSFVARFNPDQIDRA
WMGFAGGFSHAWAQARRLQTGRVRDHSLWIALGCAALLLYAWGTSWR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=447, Percent_Identity=32.662192393736, Blast_Score=203, Evalue=4e-52,
Organism=Escherichia coli, GI1788614, Length=480, Percent_Identity=37.9166666666667, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1788829, Length=416, Percent_Identity=33.1730769230769, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788831, Length=346, Percent_Identity=29.1907514450867, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788827, Length=355, Percent_Identity=26.1971830985915, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI145693160, Length=381, Percent_Identity=29.9212598425197, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI2367154, Length=289, Percent_Identity=28.3737024221453, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 71765; Mature: 71634

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLLPFVPVPALIGFVVLSLIPLALSRAVVIAIGVGAAVLPAILFAPVALSCLDGACVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GAVVPILTIDAGMFVAQIAMSLDPLSMVTGGTVSVVAALVLIYSGAYMASEPLPDLRRYF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHH
ALMNLFLAGMLAVVFAADVILLFLGWETIGLCSFMLIAFYDKLPKAVSAGRKALITTRIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DSLLLAGLMMLFLSAGTTRLDGMLAVVPGMDPWRLAPIAGLIALGALGKSAQFPFHTWLP
HHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCC
SAMAGPTPVSALLHSATMVAAGVILLARLAPLFAAAPEISAMVAMLGILTAAGGALIAVI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
QTDIKKLLAFSTMSQIGFMVLALGLGAPAVALAHFAIHAAFKSLLFLSAGVMSHASGGST
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AIEALRGARYRQPWAFWSFAIGAASLAGLPVITAGWYSKEAVLAAVWSSGPWGVPLWLLA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHEECCCCCCHHHHHHH
AAAAMLTAAYAFRVVFVAGSQSADLAAPPWGGAAVMIPLVVLTAVAVVGGLSVGALIGFA
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCC
GGTEDHLPLLPTLLGAAAPLLGVVLARQLVRHPRELEHFAKRLRHVRALRIEILYYYYIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRFRHLATVLGNAEGTEAVAQAAALRREGVDAPGLMADDPLGRAFGKEAAQPDFISRATD
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCC
GQTPVVRLVAGDPVGRILIIVARVFVGSFVARFNPDQIDRAWMGFAGGFSHAWAQARRLQ
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
TGRVRDHSLWIALGCAALLLYAWGTSWR
CCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASLLPFVPVPALIGFVVLSLIPLALSRAVVIAIGVGAAVLPAILFAPVALSCLDGACVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GAVVPILTIDAGMFVAQIAMSLDPLSMVTGGTVSVVAALVLIYSGAYMASEPLPDLRRYF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHCCCCCHHHHHHH
ALMNLFLAGMLAVVFAADVILLFLGWETIGLCSFMLIAFYDKLPKAVSAGRKALITTRIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DSLLLAGLMMLFLSAGTTRLDGMLAVVPGMDPWRLAPIAGLIALGALGKSAQFPFHTWLP
HHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCC
SAMAGPTPVSALLHSATMVAAGVILLARLAPLFAAAPEISAMVAMLGILTAAGGALIAVI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
QTDIKKLLAFSTMSQIGFMVLALGLGAPAVALAHFAIHAAFKSLLFLSAGVMSHASGGST
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AIEALRGARYRQPWAFWSFAIGAASLAGLPVITAGWYSKEAVLAAVWSSGPWGVPLWLLA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHEECCCCCCHHHHHHH
AAAAMLTAAYAFRVVFVAGSQSADLAAPPWGGAAVMIPLVVLTAVAVVGGLSVGALIGFA
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCC
GGTEDHLPLLPTLLGAAAPLLGVVLARQLVRHPRELEHFAKRLRHVRALRIEILYYYYIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRFRHLATVLGNAEGTEAVAQAAALRREGVDAPGLMADDPLGRAFGKEAAQPDFISRATD
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCC
GQTPVVRLVAGDPVGRILIIVARVFVGSFVARFNPDQIDRAWMGFAGGFSHAWAQARRLQ
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHH
TGRVRDHSLWIALGCAALLLYAWGTSWR
CCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]