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The map label for this gene is nuoM [H]

Identifier: 86748483

GI number: 86748483

Start: 1547329

End: 1548774

Strand: Direct

Name: nuoM [H]

Synonym: RPB_1358

Alternate gene names: 86748483

Gene position: 1547329-1548774 (Clockwise)

Preceding gene: 86748482

Following gene: 86748484

Centisome position: 29.02

GC content: 67.57

Gene sequence:

>1446_bases
ATGGCGCTGATCGCGCTCATTCTGCTGCCGGCCATCGGCGGCTTCGTCGCATTCTTCATCGCGGGGCGCGGTGAGCGCGA
GCGCTGGGTCACGATCGCGACCTTCGCGCTGATGCTGGTGCTGCTGATGGCGATCGTCGCCGGCGCGCCGGAAGGCCGCT
GGTACGCCAAGGTCTCCTATCCGTGGGTGCCGTCGTTCGGTATCTCGCTCGATTTCGCGATGGACGGGTTGTCGGCGGCG
CTGATCGCGATATCGGCCGGGCTCGGCATCATCTCGGTGATGGCGTCGTGGTCGGAGATCCGCACCCAGTCCGGGCTGTT
CCATGCCTGCCTGTGCTGGACCGTGGCGGCGACGATCGGCGTCTTCCTCTCGTTCGATCTGCTGATCTTCGCGTTCTTCT
GGGAAGCGATGCTGGTGCCGGCGTTCACGCTGATCGCGGTCTGGGGCCATGGCGATCGGGAAGGCGCGGCGCTGAAATTC
CTGATCTTCAATGCGGTCGCGGGCTTCGGCCTGCTGGCGGCGGCGTTTGCGCTGGCGTCGATGGCCGATCACATGACCTT
CAGCGCGTTCGAACTCGCGGAGATGAAGCTCAGTACGTCGGTGCAGGTCTGGATGCTGCTCGGCTTCTCGCTGGCGTTTC
TGGTCAAGCTGTCGGTGCCGCCGTTCCACGCCTGGCTGCCTGAGGCGCATACGCTGGCGCCGACCGCGGGCTCGATCCTG
CTCGCCGGCATCCTGCTGAAGACCGGCGCCTACGGCCTGTTCCGCTTCGCGCCGATGCTGTTTCCGCAGGGGCTCGAAGC
GGTCGCGCCCTACGGCATTGCGCTCGGCGCTGCTGGCGCGCTGTATGGCGGCCTGGTCGCCTGCGGCCAGAACGACGCCA
AGCGGCTGGTCGCCTATACCTCGATCGCCCATATGTCGATCGTGCTGATGGGCATCGCCGCGGGCGTGCACTACGCGGTC
GCCGGCGCCGCCGTGGAGATGATCGCGCATTCGTTCTCGGCCTCGGCGCTGTTCCTGCTGATTGGGGCGCTGTACGAGCG
CACCCACACCCGCGATCTGCGCCAGCTCGGTGGCCTGCAGCAGATCGCGCCGCGCTTCGCTGCGGCCTTCGCGCTGTTCT
GCTCGGCGCTGCTGGCGCTGCCCGGCACGGCGAATTTCGTCGGCGAGGCGCTGGTGGTCGTCGGCATCTTCCAGGTCAAT
TGGGTGTTCGCACTGCTGGCGCTGTCGACGCTGGTGGTCTCGGTGATCTACGCGACGCGGCTGTTGAAGGGGCTGGTGTT
CGGTCAGCCGCGGCTCGGCGCGCCGGTCGCCGACCTGACCTGGCGTGAATATGGGCCGGTGCTGGCGATGGGCCTCGCCA
CGCTGGTAATCGGCCTGTATCCGCAGAGCCTGCTGGCGCTGCTGGAGCCCGCGATCAAGGCCGCTCTGGTGCTGCCGCAG
CCATGA

Upstream 100 bases:

>100_bases
GGGCGCAGGCACGGCGGCTGCAGACCGGCCGCGTCCGCGATCATTCGCTCTGGATCGCGCTCGGCTGCGCGGCGCTTCTA
CTTTATGCTTGGGGGACGTC

Downstream 100 bases:

>100_bases
CCGCAGCCCAGTTCGCCGCGCTTTCGCCGCTCATCGCGCTGGCCATCGCCGCTGTCGCAGCGATGATGCTGGTGCCGCTG
GCGCGCATCGAAGCCGCGCA

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MALIALILLPAIGGFVAFFIAGRGERERWVTIATFALMLVLLMAIVAGAPEGRWYAKVSYPWVPSFGISLDFAMDGLSAA
LIAISAGLGIISVMASWSEIRTQSGLFHACLCWTVAATIGVFLSFDLLIFAFFWEAMLVPAFTLIAVWGHGDREGAALKF
LIFNAVAGFGLLAAAFALASMADHMTFSAFELAEMKLSTSVQVWMLLGFSLAFLVKLSVPPFHAWLPEAHTLAPTAGSIL
LAGILLKTGAYGLFRFAPMLFPQGLEAVAPYGIALGAAGALYGGLVACGQNDAKRLVAYTSIAHMSIVLMGIAAGVHYAV
AGAAVEMIAHSFSASALFLLIGALYERTHTRDLRQLGGLQQIAPRFAAAFALFCSALLALPGTANFVGEALVVVGIFQVN
WVFALLALSTLVVSVIYATRLLKGLVFGQPRLGAPVADLTWREYGPVLAMGLATLVIGLYPQSLLALLEPAIKAALVLPQ
P

Sequences:

>Translated_481_residues
MALIALILLPAIGGFVAFFIAGRGERERWVTIATFALMLVLLMAIVAGAPEGRWYAKVSYPWVPSFGISLDFAMDGLSAA
LIAISAGLGIISVMASWSEIRTQSGLFHACLCWTVAATIGVFLSFDLLIFAFFWEAMLVPAFTLIAVWGHGDREGAALKF
LIFNAVAGFGLLAAAFALASMADHMTFSAFELAEMKLSTSVQVWMLLGFSLAFLVKLSVPPFHAWLPEAHTLAPTAGSIL
LAGILLKTGAYGLFRFAPMLFPQGLEAVAPYGIALGAAGALYGGLVACGQNDAKRLVAYTSIAHMSIVLMGIAAGVHYAV
AGAAVEMIAHSFSASALFLLIGALYERTHTRDLRQLGGLQQIAPRFAAAFALFCSALLALPGTANFVGEALVVVGIFQVN
WVFALLALSTLVVSVIYATRLLKGLVFGQPRLGAPVADLTWREYGPVLAMGLATLVIGLYPQSLLALLEPAIKAALVLPQ
P
>Mature_480_residues
ALIALILLPAIGGFVAFFIAGRGERERWVTIATFALMLVLLMAIVAGAPEGRWYAKVSYPWVPSFGISLDFAMDGLSAAL
IAISAGLGIISVMASWSEIRTQSGLFHACLCWTVAATIGVFLSFDLLIFAFFWEAMLVPAFTLIAVWGHGDREGAALKFL
IFNAVAGFGLLAAAFALASMADHMTFSAFELAEMKLSTSVQVWMLLGFSLAFLVKLSVPPFHAWLPEAHTLAPTAGSILL
AGILLKTGAYGLFRFAPMLFPQGLEAVAPYGIALGAAGALYGGLVACGQNDAKRLVAYTSIAHMSIVLMGIAAGVHYAVA
GAAVEMIAHSFSASALFLLIGALYERTHTRDLRQLGGLQQIAPRFAAAFALFCSALLALPGTANFVGEALVVVGIFQVNW
VFALLALSTLVVSVIYATRLLKGLVFGQPRLGAPVADLTWREYGPVLAMGLATLVIGLYPQSLLALLEPAIKAALVLPQP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=359, Percent_Identity=32.8690807799443, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI251831117, Length=307, Percent_Identity=25.7328990228013, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=495, Percent_Identity=41.010101010101, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788827, Length=259, Percent_Identity=30.8880308880309, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788831, Length=351, Percent_Identity=27.3504273504274, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788614, Length=421, Percent_Identity=27.790973871734, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788829, Length=397, Percent_Identity=26.448362720403, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI2367154, Length=227, Percent_Identity=27.7533039647577, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI145693160, Length=362, Percent_Identity=25.6906077348066, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50827; Mature: 50696

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIALILLPAIGGFVAFFIAGRGERERWVTIATFALMLVLLMAIVAGAPEGRWYAKVSY
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PWVPSFGISLDFAMDGLSAALIAISAGLGIISVMASWSEIRTQSGLFHACLCWTVAATIG
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VFLSFDLLIFAFFWEAMLVPAFTLIAVWGHGDREGAALKFLIFNAVAGFGLLAAAFALAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MADHMTFSAFELAEMKLSTSVQVWMLLGFSLAFLVKLSVPPFHAWLPEAHTLAPTAGSIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHH
LAGILLKTGAYGLFRFAPMLFPQGLEAVAPYGIALGAAGALYGGLVACGQNDAKRLVAYT
HHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SIAHMSIVLMGIAAGVHYAVAGAAVEMIAHSFSASALFLLIGALYERTHTRDLRQLGGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QIAPRFAAAFALFCSALLALPGTANFVGEALVVVGIFQVNWVFALLALSTLVVSVIYATR
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLKGLVFGQPRLGAPVADLTWREYGPVLAMGLATLVIGLYPQSLLALLEPAIKAALVLPQ
HHHHHHCCCCCCCCCHHHCCHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC
P
C
>Mature Secondary Structure 
ALIALILLPAIGGFVAFFIAGRGERERWVTIATFALMLVLLMAIVAGAPEGRWYAKVSY
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]