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The map label for this gene is nuoB

Identifier: 86748473

GI number: 86748473

Start: 1536257

End: 1536883

Strand: Direct

Name: nuoB

Synonym: RPB_1348

Alternate gene names: 86748473

Gene position: 1536257-1536883 (Clockwise)

Preceding gene: 86748472

Following gene: 86748474

Centisome position: 28.81

GC content: 67.3

Gene sequence:

>627_bases
ATGACTGACCTGCCGCCCCATGCGCTTCAGCCGGCCGAGCCGGTCTCCGTCGAACAACACATGGCGCTGTCGAGCCTGTT
CACCACGCTCGAGGACCTGACCGCATGGTCGCGCAAGCACAGCCTGTGGCCGTTCAATTTCGGTCTGTCGTGCTGCTACG
TCGAACAGGTCACCGCGCTGACGCCGGTCTACGACCAGGCGCGGTTCGGCGCCGAAGTGATCCGCGCCTCGCCGCGGCAG
GCCGATCTGCTGGTCGTCTCCGGCACGGTGTTTCACAAGATGGCCGCGCCGCTGCTGCGGCTGTACGAGCAGATGCGGGC
GCCGCGCTGGGTGATCGCGATGGGTGCCTGCGCCTGTTCGGGCGGCATGTACGACATCTACTCGGTGGTGCAGGGCGTCG
ACCGCTTCATTCCGGTCGATGTCTATATCCCCGGCTGCCCGCCACGCCCGGAAGCGATGCTCGATGCGCTGATCATGCTG
CAGCAGCAGGTCGGCAGCGAACGCCGGCCGCTCGGCGTCACCGTCGGCAACAGTGCGGGCCTCGGCTTCGATGCGCCGCG
CCGGCGCGACGAGCGTCACGACCAACGTATGGCGCAGACCCTTCTCGATCCGCCGGAGACATTGTGA

Upstream 100 bases:

>100_bases
TCGGGCTGATCGAGGCGGCGGTATTCATCGGCGTGCTGCTGCTGGCGCTGGTCTATCTGTGGGCCGACGGCGCGCTCGAC
TGGCACAAGGAGCGCAAGCG

Downstream 100 bases:

>100_bases
GCGCAACCGACCTCGTCAGCGAACTCGGCGCGCGATTCGGAGACGCGGTGCTGGGCGAGCAGACCACGCGTGAGCGGTTT
CCGACGGTGTGGATCCGTCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTDLPPHALQPAEPVSVEQHMALSSLFTTLEDLTAWSRKHSLWPFNFGLSCCYVEQVTALTPVYDQARFGAEVIRASPRQ
ADLLVVSGTVFHKMAAPLLRLYEQMRAPRWVIAMGACACSGGMYDIYSVVQGVDRFIPVDVYIPGCPPRPEAMLDALIML
QQQVGSERRPLGVTVGNSAGLGFDAPRRRDERHDQRMAQTLLDPPETL

Sequences:

>Translated_208_residues
MTDLPPHALQPAEPVSVEQHMALSSLFTTLEDLTAWSRKHSLWPFNFGLSCCYVEQVTALTPVYDQARFGAEVIRASPRQ
ADLLVVSGTVFHKMAAPLLRLYEQMRAPRWVIAMGACACSGGMYDIYSVVQGVDRFIPVDVYIPGCPPRPEAMLDALIML
QQQVGSERRPLGVTVGNSAGLGFDAPRRRDERHDQRMAQTLLDPPETL
>Mature_207_residues
TDLPPHALQPAEPVSVEQHMALSSLFTTLEDLTAWSRKHSLWPFNFGLSCCYVEQVTALTPVYDQARFGAEVIRASPRQA
DLLVVSGTVFHKMAAPLLRLYEQMRAPRWVIAMGACACSGGMYDIYSVVQGVDRFIPVDVYIPGCPPRPEAMLDALIMLQ
QQVGSERRPLGVTVGNSAGLGFDAPRRRDERHDQRMAQTLLDPPETL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=169, Percent_Identity=48.5207100591716, Blast_Score=164, Evalue=7e-41,
Organism=Escherichia coli, GI1788624, Length=204, Percent_Identity=57.3529411764706, Blast_Score=247, Evalue=5e-67,
Organism=Escherichia coli, GI1788834, Length=135, Percent_Identity=42.962962962963, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=43.4426229508197, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=140, Percent_Identity=50.7142857142857, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI18859983, Length=153, Percent_Identity=49.6732026143791, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24642371, Length=153, Percent_Identity=49.6732026143791, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=53.5714285714286, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_RHOP2 (Q2J0F2)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484969.1
- STRING:   Q2J0F2
- GeneID:   3907856
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1348
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PEAMLDA
- ProtClustDB:   PRK06411
- BioCyc:   RPAL316058:RPB_1348-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 23082; Mature: 22951

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPPHALQPAEPVSVEQHMALSSLFTTLEDLTAWSRKHSLWPFNFGLSCCYVEQVTAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
TPVYDQARFGAEVIRASPRQADLLVVSGTVFHKMAAPLLRLYEQMRAPRWVIAMGACACS
CCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHC
GGMYDIYSVVQGVDRFIPVDVYIPGCPPRPEAMLDALIMLQQQVGSERRPLGVTVGNSAG
CCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
LGFDAPRRRDERHDQRMAQTLLDPPETL
CCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDLPPHALQPAEPVSVEQHMALSSLFTTLEDLTAWSRKHSLWPFNFGLSCCYVEQVTAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
TPVYDQARFGAEVIRASPRQADLLVVSGTVFHKMAAPLLRLYEQMRAPRWVIAMGACACS
CCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHC
GGMYDIYSVVQGVDRFIPVDVYIPGCPPRPEAMLDALIMLQQQVGSERRPLGVTVGNSAG
CCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
LGFDAPRRRDERHDQRMAQTLLDPPETL
CCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA