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The map label for this gene is rnhA

Identifier: 86748467

GI number: 86748467

Start: 1528662

End: 1529147

Strand: Direct

Name: rnhA

Synonym: RPB_1342

Alternate gene names: 86748467

Gene position: 1528662-1529147 (Clockwise)

Preceding gene: 86748466

Following gene: 86748472

Centisome position: 28.67

GC content: 64.81

Gene sequence:

>486_bases
GTGAGCGGGGCTCTGAGCGAAGCCGGGGCCGGCCCCCGCCCGGTGGTGATCCACACCGACGGTGCCTGTTCGGGCAACCC
CGGCCCCGGGGGGTGGGGGGCCATCCTCAAATTCGGCGACACCGAAAAAGAACTCAAAGGCGGCGAAGCCCATACCACGA
ACAATCGGATGGAATTGCTCGCGGCGATCTCGGCGCTGGAAGCGCTGACGCGGCCGTGCACGGTCGATCTCTACACCGAC
AGCCAGTACGTCAAGAACGGCATCGGCAGCTGGATCCACAACTGGAAGCGCAACGGCTGGAAGACCGCCGACAAGAAGCC
GGTGAAGAACGTCGATCTGTGGCAGCGCCTCGACGCCGCACTGAAGAGCCATCAGGTGCGCTGGCACTGGGTCAAGGGCC
ACGCCGGCCACGACGAAAACGAACGCGCCGATCAGTTGGCCCGGGACGGGCTCACCGAGAATCGGATGAAATCGCGGATT
GGTTGA

Upstream 100 bases:

>100_bases
TCAAGCCGAAGGATCCGCTGGAATATTTCCGCAAGCTGCGCTTCCAGCAAAATGTCGCCAGCATTCGCGATTATGGTGTC
GAAGCTGCGGGAGCGGTGGC

Downstream 100 bases:

>100_bases
GGCGGTATACGTCGTCGTGCCGTCATCCTGAGGAGCGAGCCCGAAGGGCGAGCCTCGTAGGATGCGGAAACGCGTGCAAG
CGGCCCATCCTTCGAGGCGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MSGALSEAGAGPRPVVIHTDGACSGNPGPGGWGAILKFGDTEKELKGGEAHTTNNRMELLAAISALEALTRPCTVDLYTD
SQYVKNGIGSWIHNWKRNGWKTADKKPVKNVDLWQRLDAALKSHQVRWHWVKGHAGHDENERADQLARDGLTENRMKSRI
G

Sequences:

>Translated_161_residues
MSGALSEAGAGPRPVVIHTDGACSGNPGPGGWGAILKFGDTEKELKGGEAHTTNNRMELLAAISALEALTRPCTVDLYTD
SQYVKNGIGSWIHNWKRNGWKTADKKPVKNVDLWQRLDAALKSHQVRWHWVKGHAGHDENERADQLARDGLTENRMKSRI
G
>Mature_160_residues
SGALSEAGAGPRPVVIHTDGACSGNPGPGGWGAILKFGDTEKELKGGEAHTTNNRMELLAAISALEALTRPCTVDLYTDS
QYVKNGIGSWIHNWKRNGWKTADKKPVKNVDLWQRLDAALKSHQVRWHWVKGHAGHDENERADQLARDGLTENRMKSRIG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=151, Percent_Identity=39.0728476821192, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=69.8529411764706, Blast_Score=202, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=40.1408450704225, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505482, Length=134, Percent_Identity=35.0746268656716, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=36.3013698630137, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=36.3013698630137, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_RHOP2 (Q2J0F8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484963.1
- ProteinModelPortal:   Q2J0F8
- SMR:   Q2J0F8
- STRING:   Q2J0F8
- GeneID:   3907850
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1342
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   RPAL316058:RPB_1342-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17661; Mature: 17530

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGALSEAGAGPRPVVIHTDGACSGNPGPGGWGAILKFGDTEKELKGGEAHTTNNRMELL
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHEEECCCCHHHHCCCCCCCCCHHHHHH
AAISALEALTRPCTVDLYTDSQYVKNGIGSWIHNWKRNGWKTADKKPVKNVDLWQRLDAA
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
LKSHQVRWHWVKGHAGHDENERADQLARDGLTENRMKSRIG
HHHCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
SGALSEAGAGPRPVVIHTDGACSGNPGPGGWGAILKFGDTEKELKGGEAHTTNNRMELL
CCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHEEECCCCHHHHCCCCCCCCCHHHHHH
AAISALEALTRPCTVDLYTDSQYVKNGIGSWIHNWKRNGWKTADKKPVKNVDLWQRLDAA
HHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
LKSHQVRWHWVKGHAGHDENERADQLARDGLTENRMKSRIG
HHHCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA