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The map label for this gene is ispH [H]

Identifier: 86748465

GI number: 86748465

Start: 1526621

End: 1527586

Strand: Direct

Name: ispH [H]

Synonym: RPB_1340

Alternate gene names: 86748465

Gene position: 1526621-1527586 (Clockwise)

Preceding gene: 86748463

Following gene: 86748466

Centisome position: 28.63

GC content: 67.6

Gene sequence:

>966_bases
ATGCTGGCCAAACCGCCTCTGAAGATCGTCCTTTGCTCGCCGCGCGGCTTCTGCGCCGGCGTGGTGCGGGCGATCGACAC
CGTGGAGCGTGCGCTGGCGCTGTACGGCGCCCCGGTCTACGTCCGCCACGAGATCGTCCACAACAAATACGTGGTCGACA
GCCTGCGCGCCAAGGGCGCGATTTTCGTCGAGGAACTGGGCGAGATTCCGGACACCCGGGCCCCGGTAGTGTTCTCCGCC
CATGGCGTGCCGAAATCGGTTCCGGAAGATGCGCTGCAGCGGAATTTTTTCTCGGTCGACGCCACCTGTCCGCTGGTCAC
CAAGGTGCATCGCGAGGCGGCGATCCATTTCAAGCGCGGCCGCGAGATCCTGCTGATCGGGCATTCGCATCACCCGGAAG
TGGTCGGCACGCTGGGCCAGCTTCCCCCCGGCGCGGTGACACTGATCGAGACCGCCGCCGACGCCCAGAGCTTCACCCCG
AAGAACCCGGACAATCTCGCTTTCGTGACCCAGACCACGCTGTCGATCGACGACACCGCCGGCATCGTGGCGATCCTGCG
CCAGCGCTTCCCGAACATCTCCGGGCCGCACAAGGAAGACATCTGCTACGCCACCACCAACCGCCAGGCGGCGGTGAAGA
AGGTGGCGCCGGTGGTCGATGCGATGATCGTGGTCGGTGCGCCGAATTCGTCGAATTCGCAGCGGCTGCGCGAGGTCGCC
GAGCGCGAGGGCTGCCGGGTCTCGGTGCTGGCGCAGCGCGCCGCCGATATCGACTGGGCGCAATTCGAGGGCATCACCAG
CCTCGGCCTCACCGCCGGCGCCTCCGCCCCGGAGGTGATCGTCGAGGAGATCATGGGCGCCTTCGCCGAGCGCTTCGAGC
TCAGCGTCGAGACGGTGTCCGCGGCGGAGGAGAACGAGTTCTTCCCGCTGCCGCGCGTGCTGCGCCCGGACGCCGCCGCC
GAATAG

Upstream 100 bases:

>100_bases
TCCCAATTCCTTGGTCAAGACGAACCATCGCAACGCCCGCAGGGCGTTGGCCGCTACCGCCGTTCGCGGCGTGCGGGCAG
CCTCCCCGGAACGCGCAAAA

Downstream 100 bases:

>100_bases
CGGTGGGGAAGGGGCCGGCCCCGGGGGCCCGCCCTTTCATTGCCGGGCGCGCTGGTCTAGGACACCGCCATCACTCCTCA
CCACCCGGGACGCCCCCCAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MLAKPPLKIVLCSPRGFCAGVVRAIDTVERALALYGAPVYVRHEIVHNKYVVDSLRAKGAIFVEELGEIPDTRAPVVFSA
HGVPKSVPEDALQRNFFSVDATCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTLGQLPPGAVTLIETAADAQSFTP
KNPDNLAFVTQTTLSIDDTAGIVAILRQRFPNISGPHKEDICYATTNRQAAVKKVAPVVDAMIVVGAPNSSNSQRLREVA
EREGCRVSVLAQRAADIDWAQFEGITSLGLTAGASAPEVIVEEIMGAFAERFELSVETVSAAEENEFFPLPRVLRPDAAA
E

Sequences:

>Translated_321_residues
MLAKPPLKIVLCSPRGFCAGVVRAIDTVERALALYGAPVYVRHEIVHNKYVVDSLRAKGAIFVEELGEIPDTRAPVVFSA
HGVPKSVPEDALQRNFFSVDATCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTLGQLPPGAVTLIETAADAQSFTP
KNPDNLAFVTQTTLSIDDTAGIVAILRQRFPNISGPHKEDICYATTNRQAAVKKVAPVVDAMIVVGAPNSSNSQRLREVA
EREGCRVSVLAQRAADIDWAQFEGITSLGLTAGASAPEVIVEEIMGAFAERFELSVETVSAAEENEFFPLPRVLRPDAAA
E
>Mature_321_residues
MLAKPPLKIVLCSPRGFCAGVVRAIDTVERALALYGAPVYVRHEIVHNKYVVDSLRAKGAIFVEELGEIPDTRAPVVFSA
HGVPKSVPEDALQRNFFSVDATCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTLGQLPPGAVTLIETAADAQSFTP
KNPDNLAFVTQTTLSIDDTAGIVAILRQRFPNISGPHKEDICYATTNRQAAVKKVAPVVDAMIVVGAPNSSNSQRLREVA
EREGCRVSVLAQRAADIDWAQFEGITSLGLTAGASAPEVIVEEIMGAFAERFELSVETVSAAEENEFFPLPRVLRPDAAA
E

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=313, Percent_Identity=51.1182108626198, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34610; Mature: 34610

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKPPLKIVLCSPRGFCAGVVRAIDTVERALALYGAPVYVRHEIVHNKYVVDSLRAKGA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCC
IFVEELGEIPDTRAPVVFSAHGVPKSVPEDALQRNFFSVDATCPLVTKVHREAAIHFKRG
CHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCC
REILLIGHSHHPEVVGTLGQLPPGAVTLIETAADAQSFTPKNPDNLAFVTQTTLSIDDTA
CEEEEEECCCCCHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCEEEEEEEEEECCCHH
GIVAILRQRFPNISGPHKEDICYATTNRQAAVKKVAPVVDAMIVVGAPNSSNSQRLREVA
HHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHH
EREGCRVSVLAQRAADIDWAQFEGITSLGLTAGASAPEVIVEEIMGAFAERFELSVETVS
HHCCCEEEEEHHHHCCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAEENEFFPLPRVLRPDAAAE
CCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MLAKPPLKIVLCSPRGFCAGVVRAIDTVERALALYGAPVYVRHEIVHNKYVVDSLRAKGA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCC
IFVEELGEIPDTRAPVVFSAHGVPKSVPEDALQRNFFSVDATCPLVTKVHREAAIHFKRG
CHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCC
REILLIGHSHHPEVVGTLGQLPPGAVTLIETAADAQSFTPKNPDNLAFVTQTTLSIDDTA
CEEEEEECCCCCHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCEEEEEEEEEECCCHH
GIVAILRQRFPNISGPHKEDICYATTNRQAAVKKVAPVVDAMIVVGAPNSSNSQRLREVA
HHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHH
EREGCRVSVLAQRAADIDWAQFEGITSLGLTAGASAPEVIVEEIMGAFAERFELSVETVS
HHCCCEEEEEHHHHCCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAEENEFFPLPRVLRPDAAAE
CCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA