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The map label for this gene is hycG [H]

Identifier: 86748390

GI number: 86748390

Start: 1448387

End: 1448920

Strand: Direct

Name: hycG [H]

Synonym: RPB_1265

Alternate gene names: 86748390

Gene position: 1448387-1448920 (Clockwise)

Preceding gene: 86748389

Following gene: 86748391

Centisome position: 27.17

GC content: 68.54

Gene sequence:

>534_bases
ATGCGCAAGCTGCTGTTCGAAAGCCTGTTCGCCAAGCCGTTGACGGAAAGCCCGCCGGCGCCGGACGATGCCGCCGTCGC
CGAACTCGCCGCGCGGCTCGACGCGACCGCGCGGCGGATGCTCGGCCGCAGCCTGTCGATCCGCGAGGTCGACGCCGGCT
CCTGCAATGGTTGCGAACTCGAGATCCACGCGCTCAACAATGCGTATTACGACGTCGAACGGTTCGGCCTGCGCTTCGTC
GCCTCGCCGCGCCATGCCGACGTGCTGATGGTCACCGGGCCGGTGACGCACAACATGCGCGAGGCGCTGCGGCTCACTTA
CGACGCGGTGCCCGGACCGAAATGGGTGGTCGCGGTCGGCGATTGCGCCAAAGACGGCGGTTGCTTCGCCGGCAGCTACG
CGGTCGCCGGCGGCGTCTCCGCGGTGATCCCGGTCGATCTGCACATCCCCGGCTGCCCGCCATCACCTATGCAAATCCTG
CGCGGCCTGCTGGCGCTTCTCGATAGCGTCTCCGAGCGCGAGACGTCGCGTTGA

Upstream 100 bases:

>100_bases
GGCCGCTGCTGGAAGCCGTGATCGAGGGCAACATCATCGCCGACTTCCCGCTGTGCAACAAATCGTTCAACTGCTCTTAT
TCGGGCGTGGATCTGTAGCG

Downstream 100 bases:

>100_bases
CATAAGCCGATACCGCGAGTTCTCGTTTTTCATCGCGATGTCATCAGCCGCGCGACTAATGACTGGTGTGTTCAGTATTT
CTATCAGTTGCTGCTGCCCC

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MRKLLFESLFAKPLTESPPAPDDAAVAELAARLDATARRMLGRSLSIREVDAGSCNGCELEIHALNNAYYDVERFGLRFV
ASPRHADVLMVTGPVTHNMREALRLTYDAVPGPKWVVAVGDCAKDGGCFAGSYAVAGGVSAVIPVDLHIPGCPPSPMQIL
RGLLALLDSVSERETSR

Sequences:

>Translated_177_residues
MRKLLFESLFAKPLTESPPAPDDAAVAELAARLDATARRMLGRSLSIREVDAGSCNGCELEIHALNNAYYDVERFGLRFV
ASPRHADVLMVTGPVTHNMREALRLTYDAVPGPKWVVAVGDCAKDGGCFAGSYAVAGGVSAVIPVDLHIPGCPPSPMQIL
RGLLALLDSVSERETSR
>Mature_177_residues
MRKLLFESLFAKPLTESPPAPDDAAVAELAARLDATARRMLGRSLSIREVDAGSCNGCELEIHALNNAYYDVERFGLRFV
ASPRHADVLMVTGPVTHNMREALRLTYDAVPGPKWVVAVGDCAKDGGCFAGSYAVAGGVSAVIPVDLHIPGCPPSPMQIL
RGLLALLDSVSERETSR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=115, Percent_Identity=42.6086956521739, Blast_Score=109, Evalue=1e-24,
Organism=Escherichia coli, GI1789074, Length=157, Percent_Identity=40.1273885350318, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788834, Length=131, Percent_Identity=41.9847328244275, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1788624, Length=133, Percent_Identity=39.8496240601504, Blast_Score=94, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=165, Percent_Identity=38.1818181818182, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18859983, Length=161, Percent_Identity=40.3726708074534, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24642371, Length=161, Percent_Identity=40.3726708074534, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=39.7260273972603, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 18917; Mature: 18917

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLFESLFAKPLTESPPAPDDAAVAELAARLDATARRMLGRSLSIREVDAGSCNGCEL
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEE
EIHALNNAYYDVERFGLRFVASPRHADVLMVTGPVTHNMREALRLTYDAVPGPKWVVAVG
EEEECCCCHHHHHHHCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEC
DCAKDGGCFAGSYAVAGGVSAVIPVDLHIPGCPPSPMQILRGLLALLDSVSERETSR
CCCCCCCEEECCHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKLLFESLFAKPLTESPPAPDDAAVAELAARLDATARRMLGRSLSIREVDAGSCNGCEL
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEE
EIHALNNAYYDVERFGLRFVASPRHADVLMVTGPVTHNMREALRLTYDAVPGPKWVVAVG
EEEECCCCHHHHHHHCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEC
DCAKDGGCFAGSYAVAGGVSAVIPVDLHIPGCPPSPMQILRGLLALLDSVSERETSR
CCCCCCCEEECCHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]