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The map label for this gene is hycE [H]

Identifier: 86748389

GI number: 86748389

Start: 1446873

End: 1448384

Strand: Direct

Name: hycE [H]

Synonym: RPB_1264

Alternate gene names: 86748389

Gene position: 1446873-1448384 (Clockwise)

Preceding gene: 86748388

Following gene: 86748390

Centisome position: 27.14

GC content: 68.06

Gene sequence:

>1512_bases
ATGCCGTCGCTGATCGATCTCAACCTCGAAGGCCGGCAAGTGGCCCGGCATCGGCCGTGGAGCCGCACGGTGGTCGACAG
CGCGGTGTGGAGCTTCGCGGTCAAAATGCTGAGCGAGCGGCAGTGGCAATTGCTGTCGCTGTGGGGCGAGCCGGCGGCCG
TGCACATGGCGCTGTTCGATCCGCGCACCGCCGAGATCGGACTCATCAGCCTGGATTGTCCGCATGGTTCATTTCCATCG
GTCGCCGCCAAGCATCTGCCGGCGCTGCGGCTGGAGCGCACGATCACCGATCTGTTCGGCCTGAAGCCCGACGGTGCGAT
CGATGATCGGCCGTGGCTCGATCACGGCCAATGGGGCGTCCACGCGCCGCTCGGCGCGCGCGTCCAAGCTGAGACGCCGC
CGCCGGCCTATCGCTTCCTGCCGGTGCATGGCGAGGGCGCGCATCAGGTTGCGGTCGGCCCGGTGCATGCCGGCATCATC
GAGCCGGGGCACTTCCGGTTCACCGTCAGCGGCGAAACCGTGGTGCGGCTGGAGCAGCGGCTCGGCTATGTCCACAAGGG
CATCGACGGCCTGATCGCCGGCGCGGATTTGGCAAGCGCTGCACGTCTCGCCGGCCGCGTCTCCGGCGATTCGACGGTGG
CTTATGCGCTGGCGTTTTCGCGCGCGGTGGAAGCTGCCGCCGAGATCGTGGTTCCGGCCCGCGCGGTGTGGCTGCGGGCG
CTCGTCGCCGAGCTCGAGCGTCTCGCCAATCACCTCGGCGACATCGGCGCGGTCTGCAACGACGCCGCTTTTGCGATCAT
GCTGGCGCAGTTCGGCGTGCTGCGCGAACACGTGCTGCGCGCCGCTGACGCTGCGTTCGGCCATCGCCTGATGCGTGACG
TGGTGATGCCCGGCGGCGTCGCGCGCGATCTCGACGACAATGGCAAGGCCGCGTTCCGCGATGCGCTCGCCGCCGTGCGC
GCCAAGCTGCCGGCGCTGATTGAACTCTACGACAGCACCGCCTCGCTGCAGGACCGCACCGTGTCGACGGGCATCCTCAG
GCCCGCGCTCGCCGATCAATACGCCGCCGGCGGCTTTGTCGGCCGTGCCTCCGGCCGCGGCTTCGATGCGCGCCGCACGC
TGGCTTACGCGCCCTATCATCAACTGCGCTTCGACGTACCGGTGCTGACCGAGGGCGACGTCGACGCCCGCATCTGGATC
CGGATTCGCGAGGTCGAGCAGAGCCTGTCGCTGGTCGACCAGATTCTCGACCGGCTGCCGCGCGGCGAGATCCGCGTCGC
GCCGGAATTGCCGGACCAGGCCTGCGAAGGCCTGGCGCTGATCGAAGGGTTTCGCGGCGATATTCTGGTCTGGCTGCGGT
TGCAGGACGGCGTCGTCGCGCGCTGTCACTTGCGCGATCCGTCGTGGTTTCAATGGCCGCTGCTGGAAGCCGTGATCGAG
GGCAACATCATCGCCGACTTCCCGCTGTGCAACAAATCGTTCAACTGCTCTTATTCGGGCGTGGATCTGTAG

Upstream 100 bases:

>100_bases
TCTGGCGCTGGTGCTGATCGCCGGCGTGTATCTGCCGGCGCCGGTGGTGGCGTGGTTCCAGCACGTCGCGAAACTGCTGG
GCTAGGGAGCAACAGATCCG

Downstream 100 bases:

>100_bases
CGATGCGCAAGCTGCTGTTCGAAAGCCTGTTCGCCAAGCCGTTGACGGAAAGCCCGCCGGCGCCGGACGATGCCGCCGTC
GCCGAACTCGCCGCGCGGCT

Product: NADH-ubiquinone oxidoreductase, chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MPSLIDLNLEGRQVARHRPWSRTVVDSAVWSFAVKMLSERQWQLLSLWGEPAAVHMALFDPRTAEIGLISLDCPHGSFPS
VAAKHLPALRLERTITDLFGLKPDGAIDDRPWLDHGQWGVHAPLGARVQAETPPPAYRFLPVHGEGAHQVAVGPVHAGII
EPGHFRFTVSGETVVRLEQRLGYVHKGIDGLIAGADLASAARLAGRVSGDSTVAYALAFSRAVEAAAEIVVPARAVWLRA
LVAELERLANHLGDIGAVCNDAAFAIMLAQFGVLREHVLRAADAAFGHRLMRDVVMPGGVARDLDDNGKAAFRDALAAVR
AKLPALIELYDSTASLQDRTVSTGILRPALADQYAAGGFVGRASGRGFDARRTLAYAPYHQLRFDVPVLTEGDVDARIWI
RIREVEQSLSLVDQILDRLPRGEIRVAPELPDQACEGLALIEGFRGDILVWLRLQDGVVARCHLRDPSWFQWPLLEAVIE
GNIIADFPLCNKSFNCSYSGVDL

Sequences:

>Translated_503_residues
MPSLIDLNLEGRQVARHRPWSRTVVDSAVWSFAVKMLSERQWQLLSLWGEPAAVHMALFDPRTAEIGLISLDCPHGSFPS
VAAKHLPALRLERTITDLFGLKPDGAIDDRPWLDHGQWGVHAPLGARVQAETPPPAYRFLPVHGEGAHQVAVGPVHAGII
EPGHFRFTVSGETVVRLEQRLGYVHKGIDGLIAGADLASAARLAGRVSGDSTVAYALAFSRAVEAAAEIVVPARAVWLRA
LVAELERLANHLGDIGAVCNDAAFAIMLAQFGVLREHVLRAADAAFGHRLMRDVVMPGGVARDLDDNGKAAFRDALAAVR
AKLPALIELYDSTASLQDRTVSTGILRPALADQYAAGGFVGRASGRGFDARRTLAYAPYHQLRFDVPVLTEGDVDARIWI
RIREVEQSLSLVDQILDRLPRGEIRVAPELPDQACEGLALIEGFRGDILVWLRLQDGVVARCHLRDPSWFQWPLLEAVIE
GNIIADFPLCNKSFNCSYSGVDL
>Mature_502_residues
PSLIDLNLEGRQVARHRPWSRTVVDSAVWSFAVKMLSERQWQLLSLWGEPAAVHMALFDPRTAEIGLISLDCPHGSFPSV
AAKHLPALRLERTITDLFGLKPDGAIDDRPWLDHGQWGVHAPLGARVQAETPPPAYRFLPVHGEGAHQVAVGPVHAGIIE
PGHFRFTVSGETVVRLEQRLGYVHKGIDGLIAGADLASAARLAGRVSGDSTVAYALAFSRAVEAAAEIVVPARAVWLRAL
VAELERLANHLGDIGAVCNDAAFAIMLAQFGVLREHVLRAADAAFGHRLMRDVVMPGGVARDLDDNGKAAFRDALAAVRA
KLPALIELYDSTASLQDRTVSTGILRPALADQYAAGGFVGRASGRGFDARRTLAYAPYHQLRFDVPVLTEGDVDARIWIR
IREVEQSLSLVDQILDRLPRGEIRVAPELPDQACEGLALIEGFRGDILVWLRLQDGVVARCHLRDPSWFQWPLLEAVIEG
NIIADFPLCNKSFNCSYSGVDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=347, Percent_Identity=27.3775216138329, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4758786, Length=347, Percent_Identity=27.3775216138329, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789076, Length=447, Percent_Identity=31.0961968680089, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1788832, Length=443, Percent_Identity=29.5711060948081, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI145693162, Length=472, Percent_Identity=22.8813559322034, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17555284, Length=379, Percent_Identity=26.9129287598945, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568379, Length=379, Percent_Identity=26.6490765171504, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24638644, Length=400, Percent_Identity=25, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI221459469, Length=277, Percent_Identity=28.158844765343, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 54821; Mature: 54690

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLIDLNLEGRQVARHRPWSRTVVDSAVWSFAVKMLSERQWQLLSLWGEPAAVHMALFD
CCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEHHHHCCCCCEEEEEEEC
PRTAEIGLISLDCPHGSFPSVAAKHLPALRLERTITDLFGLKPDGAIDDRPWLDHGQWGV
CCCCEEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
HAPLGARVQAETPPPAYRFLPVHGEGAHQVAVGPVHAGIIEPGHFRFTVSGETVVRLEQR
CCCCCCEEECCCCCCCEEEEEECCCCCCEEEECCEEECCCCCCCEEEEECCCHHHHHHHH
LGYVHKGIDGLIAGADLASAARLAGRVSGDSTVAYALAFSRAVEAAAEIVVPARAVWLRA
HHHHHCCHHHHEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAELERLANHLGDIGAVCNDAAFAIMLAQFGVLREHVLRAADAAFGHRLMRDVVMPGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARDLDDNGKAAFRDALAAVRAKLPALIELYDSTASLQDRTVSTGILRPALADQYAAGGFV
CCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHCCCEE
GRASGRGFDARRTLAYAPYHQLRFDVPVLTEGDVDARIWIRIREVEQSLSLVDQILDRLP
ECCCCCCCCHHCEEEECCHHHHEECCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCC
RGEIRVAPELPDQACEGLALIEGFRGDILVWLRLQDGVVARCHLRDPSWFQWPLLEAVIE
CCCEEECCCCCHHHHCCHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHC
GNIIADFPLCNKSFNCSYSGVDL
CCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSLIDLNLEGRQVARHRPWSRTVVDSAVWSFAVKMLSERQWQLLSLWGEPAAVHMALFD
CCEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEHHHHCCCCCEEEEEEEC
PRTAEIGLISLDCPHGSFPSVAAKHLPALRLERTITDLFGLKPDGAIDDRPWLDHGQWGV
CCCCEEEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
HAPLGARVQAETPPPAYRFLPVHGEGAHQVAVGPVHAGIIEPGHFRFTVSGETVVRLEQR
CCCCCCEEECCCCCCCEEEEEECCCCCCEEEECCEEECCCCCCCEEEEECCCHHHHHHHH
LGYVHKGIDGLIAGADLASAARLAGRVSGDSTVAYALAFSRAVEAAAEIVVPARAVWLRA
HHHHHCCHHHHEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAELERLANHLGDIGAVCNDAAFAIMLAQFGVLREHVLRAADAAFGHRLMRDVVMPGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARDLDDNGKAAFRDALAAVRAKLPALIELYDSTASLQDRTVSTGILRPALADQYAAGGFV
CCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHCCCEE
GRASGRGFDARRTLAYAPYHQLRFDVPVLTEGDVDARIWIRIREVEQSLSLVDQILDRLP
ECCCCCCCCHHCEEEECCHHHHEECCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHCC
RGEIRVAPELPDQACEGLALIEGFRGDILVWLRLQDGVVARCHLRDPSWFQWPLLEAVIE
CCCEEECCCCCHHHHCCHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHC
GNIIADFPLCNKSFNCSYSGVDL
CCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]