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The map label for this gene is hyfF [H]

Identifier: 86748388

GI number: 86748388

Start: 1445406

End: 1446857

Strand: Direct

Name: hyfF [H]

Synonym: RPB_1263

Alternate gene names: 86748388

Gene position: 1445406-1446857 (Clockwise)

Preceding gene: 86748387

Following gene: 86748389

Centisome position: 27.11

GC content: 66.39

Gene sequence:

>1452_bases
ATGAATTTGTCCGGCCTCGATCCGGTGGTGGGGGTGCTGCTGATCCCGGTCTGCGCCGCCATCGTGCTGGCGCTGCTGCC
GGGCTACAGGCTGACCGCGCGGCTCAACGTCGTCGCGAGTCTGGGGAGCTTTCTCTGCGCCGCCTCGCTGCTGGTGCTGC
CGCGGCCGCTACCGGGCCCGTACATCCTGGTCGACGATCTCAACATCGTCTTCATCGTGCTCAACACCTTCGTCGGCTTC
ACCACCAGCCTGTTCAGCGCCAGCTACATCGCCCACGAACTCGAAACCGGCAAGCTGACGCCGACCTATCTGCGATTCTA
CCACGCGATGTACCAGATCATGATGTTCGGCATGAATCTGGCGTTCATGTCGAACAATATCGGGCTGATGTGGGTCGCGG
TCGAACTGGCGACGCTGACCACGGTGCTGATGGTGGGGATCTATCGCTCGCCGGCGGCGCTGGAGGCGGCCTGGAAGTAC
TTTATCCTCGGCAGCGTCGGCATCGCGCTGGCGCTGTTCGGCACCATCCTGGTCTACATGGCGGCGCGGCCGGTGGTCGG
CGAGGGCCAGGACGGCATGATCTGGACCGTGCTGGTGGCGCGCGCCGCGGGCTTCGATGCGCCGCTGTTGAACGTCGCCT
TCGTGTTCCTGTTCCTCGGCTACGGCACCAAGGTCGGGCTGGCGCCGCTGCACGCCTGGCTGCCGGACGCCCACGCCGAG
GGCCCGACGCCGATCTCGGCGGTGCTGTCGGGCCTGTTGCTCAACGTGGCGCTGTACGCGCTGCTGCGCTTCAAAATGCT
GCTCGCCGCCAATGGCGACGCGATGCCGCCCGGCGTGCTGATGATCGTGATGGGGCTGGCGTCGCTCTTGTTCGCCGCCT
TCATGCTGTATCGGCGGCGCGACATCAAACGGCTGTTCGCCTATTCGTCGATCGAGCACATGGGCATCATCGTGTTCGCC
TTCGGCATGGGCGGACCGCTGGCGAATTTCGCCGGGCTCCTGCACATGGTGATGCACAGCCTGACCAAATCGGCGATCTT
CTTCGCGGTCGGCCACATCACCCAGGTCAAGGGCACGCAGTCGCTGGCGAAGATCCGCGGCATCACCGAGACCCATCCGG
TGCTCGGCTGGGGCCTCGTCGTCGGCGTGGCGGCGATTGCCGGGTTGCCGCCGCTCGGCATCTTCATGAGCGAGTTTCTG
CTGGTGTCCTCGACCTTCGCGCGGGCGCCTTGGCTGGCGCTGCTGCTGGTGTTCGGCATTCTGGTGGCGTTCGGCGCGCT
GATGCTGCGGCTGACCGGCATCGCCTTCGGCCGGCCGCGCGGCAGCCGGCAGCCGGTGCAGGCGTCCTATGTGCCGATGT
TCGCGCATCTGGCGCTGGTGCTGATCGCCGGCGTGTATCTGCCGGCGCCGGTGGTGGCGTGGTTCCAGCACGTCGCGAAA
CTGCTGGGCTAG

Upstream 100 bases:

>100_bases
TGATCGCCTTCATCGTGATCGGCATCGTGCTGTTCCGGATCCGCGAACGCTTCGACTCGGTCGACGTGGCCCAGCTCGAC
GTCTCGCGCGGAGAGCAGCG

Downstream 100 bases:

>100_bases
GGAGCAACAGATCCGATGCCGTCGCTGATCGATCTCAACCTCGAAGGCCGGCAAGTGGCCCGGCATCGGCCGTGGAGCCG
CACGGTGGTCGACAGCGCGG

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNLSGLDPVVGVLLIPVCAAIVLALLPGYRLTARLNVVASLGSFLCAASLLVLPRPLPGPYILVDDLNIVFIVLNTFVGF
TTSLFSASYIAHELETGKLTPTYLRFYHAMYQIMMFGMNLAFMSNNIGLMWVAVELATLTTVLMVGIYRSPAALEAAWKY
FILGSVGIALALFGTILVYMAARPVVGEGQDGMIWTVLVARAAGFDAPLLNVAFVFLFLGYGTKVGLAPLHAWLPDAHAE
GPTPISAVLSGLLLNVALYALLRFKMLLAANGDAMPPGVLMIVMGLASLLFAAFMLYRRRDIKRLFAYSSIEHMGIIVFA
FGMGGPLANFAGLLHMVMHSLTKSAIFFAVGHITQVKGTQSLAKIRGITETHPVLGWGLVVGVAAIAGLPPLGIFMSEFL
LVSSTFARAPWLALLLVFGILVAFGALMLRLTGIAFGRPRGSRQPVQASYVPMFAHLALVLIAGVYLPAPVVAWFQHVAK
LLG

Sequences:

>Translated_483_residues
MNLSGLDPVVGVLLIPVCAAIVLALLPGYRLTARLNVVASLGSFLCAASLLVLPRPLPGPYILVDDLNIVFIVLNTFVGF
TTSLFSASYIAHELETGKLTPTYLRFYHAMYQIMMFGMNLAFMSNNIGLMWVAVELATLTTVLMVGIYRSPAALEAAWKY
FILGSVGIALALFGTILVYMAARPVVGEGQDGMIWTVLVARAAGFDAPLLNVAFVFLFLGYGTKVGLAPLHAWLPDAHAE
GPTPISAVLSGLLLNVALYALLRFKMLLAANGDAMPPGVLMIVMGLASLLFAAFMLYRRRDIKRLFAYSSIEHMGIIVFA
FGMGGPLANFAGLLHMVMHSLTKSAIFFAVGHITQVKGTQSLAKIRGITETHPVLGWGLVVGVAAIAGLPPLGIFMSEFL
LVSSTFARAPWLALLLVFGILVAFGALMLRLTGIAFGRPRGSRQPVQASYVPMFAHLALVLIAGVYLPAPVVAWFQHVAK
LLG
>Mature_483_residues
MNLSGLDPVVGVLLIPVCAAIVLALLPGYRLTARLNVVASLGSFLCAASLLVLPRPLPGPYILVDDLNIVFIVLNTFVGF
TTSLFSASYIAHELETGKLTPTYLRFYHAMYQIMMFGMNLAFMSNNIGLMWVAVELATLTTVLMVGIYRSPAALEAAWKY
FILGSVGIALALFGTILVYMAARPVVGEGQDGMIWTVLVARAAGFDAPLLNVAFVFLFLGYGTKVGLAPLHAWLPDAHAE
GPTPISAVLSGLLLNVALYALLRFKMLLAANGDAMPPGVLMIVMGLASLLFAAFMLYRRRDIKRLFAYSSIEHMGIIVFA
FGMGGPLANFAGLLHMVMHSLTKSAIFFAVGHITQVKGTQSLAKIRGITETHPVLGWGLVVGVAAIAGLPPLGIFMSEFL
LVSSTFARAPWLALLLVFGILVAFGALMLRLTGIAFGRPRGSRQPVQASYVPMFAHLALVLIAGVYLPAPVVAWFQHVAK
LLG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=217, Percent_Identity=31.7972350230415, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI251831116, Length=256, Percent_Identity=25.390625, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788831, Length=420, Percent_Identity=38.0952380952381, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788827, Length=396, Percent_Identity=28.7878787878788, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788613, Length=432, Percent_Identity=25.6944444444444, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788829, Length=429, Percent_Identity=26.8065268065268, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693160, Length=306, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788614, Length=351, Percent_Identity=27.0655270655271, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367154, Length=435, Percent_Identity=24.1379310344828, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51736; Mature: 51736

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHC
>Mature Secondary Structure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HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA