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The map label for this gene is hyfB [H]

Identifier: 86748385

GI number: 86748385

Start: 1441778

End: 1443796

Strand: Direct

Name: hyfB [H]

Synonym: RPB_1260

Alternate gene names: 86748385

Gene position: 1441778-1443796 (Clockwise)

Preceding gene: 86748383

Following gene: 86748386

Centisome position: 27.04

GC content: 68.45

Gene sequence:

>2019_bases
ATGACTGAACTTGCGGTTCAGCTCTGGTGCGTGGCGGTGCTGCTCGCGATCGCGGTCATCGCGGTTCCGCTGAGCCGGAG
GCCGGGCCTCGCCACCGCAACCATCTATTCCGCGACGCTGGTCACCTGCGCGGTCGCCACCGCCAGCGCACTGACCTGGC
TGCTGCTGCATGCGGACGCCGCCGCCCAGCGCACGCTGCCGATCGGGCTGCCCTGGCTCGGCGCGCATCTGCGGCTCGAT
GCGCTCGCGGCGTTCTTCCTCGTCGTGGTCAATCTCGGCGGCACGCTGGCGAGCCTGTATGCGCTCGGCTATGGCCGGCA
CGAGCACGCGCCGCATCGCGTGCTGCCGTTCTATCCGGCGTTTCTGGCCGGCATGAACATGGTGGTGCTCGCCGCCGACG
CGTTTTCTTATCTGCTGAGCTGGGAATTCATGTCGCTGGCGTCGTGGGCGCTGGTGATGGCGCATCACCGCGACGGATCC
AATCGCCGCGCCGGCTATGTCTATCTGCTGATGGCGAGTTTCGGCACGCTGGCGCTGCTCTTGGCGTTCGGCCTGCTCGC
GGGCGCCGCCGGAAATTACGATTTCGCCGCGATCCGCGCCGCGCAGCACGCGCCGTGGATCGCGACGCTGGTGCTGATCC
TGCTGCTGCTCGGCGCCGGCTCCAAGGCCGGGCTTGTCCCCTTGCATGTCTGGTTGCCGCTGGCGCATCCGGCGGCGCCG
AGCCACGTCTCGGCGCTGATGAGCGGCGTGATGACCAAGGTCGCGGTCTACGGCTTCATCCGCGTGGTGTTCGATCTGCT
CGGCACGCCGGACTGGTCGACCTCCGGGATCGTGCTGGCGCTCGGCGGCATCACGGCGGCGCTCGGCATTCTTTATGCGC
TGATGGAGAAGGACCTGAAGCGGCTGCTGGCCTATTCGACGATCGAGAATGTCGGCATCATCTTCGTCAGCCTCGGCCTC
GCGCTGGCGTTCCAGGCCAATCATCAGCCGCTGCCGGCGGCGCTGGCCTTCACCGCGGCGCTGTTCCACGTGCTCAACCA
TTCGCTGTTCAAGAGCCTGCTGTTCTTCGGCGCCGGCGCGGTGCTGAGCGCCACCGGCGAGCGCGACATGGACAAGCTCG
GCGGACTGATCCATCGCATGCCGGTGACCAGCGTGGTGTTCCTGATCGGCAGCGTCGCGATCTCGGCGCTGCCGCCGTTC
AACGGCTTCGTCTCGGAATGGCTGATCTTCCAGGCGGTGCTGCAGAGCCCGCAACTGCCGCAATGGGGATTGAAAATTCT
GGTGCCGGCGGTCGGCGGCTTGATGGCGCTCGCGGCGGCGTTGGCGGCGGCGTGTTTCGTCAAGGCCTATGGCGTGACGT
TCCTCGGCCGGCCGCGCGGCGCCGCGACGCTGGCTGCCAAAGAGGTCGATCGCTTCTCGCTCGCCGCGATGGCGATCCTC
GCCGCGCTGTGTCTCGCCGCCGGCGTGCTGCCCGGACTGGTGATGGACACGCTGGCGCCGATCGCGACCGAGATTCTCGG
CAGCCGGCTGGCGCCACAAACGCCGCTCGCCTGGCTGTCGATCGTGCCGATCGCGCAAAGCCACAGCTCCTATAATGGCC
TGCTGGTCGCATTGTTCATCGCGGTGTCGGCGTCGTTGCTGTTCGTCGCGATCCGGCTGTTCTCGTCGGGCGCGGTCCGC
CGCGGCCCGGCCTGGGGCTGCGGCTTCACCGAGCCTGCGCCGCTCGCGCAATATTCCGCGGCCGGCTTCGCGCAGCCGAT
CCGCCGGGTGTTCGGCACGCTGATCTTCCGCGCCCGCGATCACGTCACGATGCCGCCGCCGGGCGACACGGCGCCGGCGC
GGCTGCGGATCGAACTGCACGACCAGATCTGGGAGCGGATCTACGCGCCGCTCGCCGGCGGCGTCATGGCCGCCTCGGTG
CGCCTCAACCGGATGCAGCTCCTCACCATCCGCGGCTATCTCAGCCTGGTGTTCGCCACCCTCGTCACGCTGCTGCTGGT
GCTCGCGATATGGACGTGA

Upstream 100 bases:

>100_bases
TGCGAATGGGGGCACGATCGCGGATGAAGGTTCCGGCGCGGGATGGCACGCAGGCTCTCCTCGGGCGCCATATCGCTGGC
GCGGCGAGGGTGCACGCCCG

Downstream 100 bases:

>100_bases
TCACCGACATCGCCGTGCAGGGCGTGCAGATGCTGCTGGTGTTCCTGCTGGCGCCGCTGCTCACCGGCTTCATCCGCAAG
GTCAAGGCGCGGCTGCAGCG

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTELAVQLWCVAVLLAIAVIAVPLSRRPGLATATIYSATLVTCAVATASALTWLLLHADAAAQRTLPIGLPWLGAHLRLD
ALAAFFLVVVNLGGTLASLYALGYGRHEHAPHRVLPFYPAFLAGMNMVVLAADAFSYLLSWEFMSLASWALVMAHHRDGS
NRRAGYVYLLMASFGTLALLLAFGLLAGAAGNYDFAAIRAAQHAPWIATLVLILLLLGAGSKAGLVPLHVWLPLAHPAAP
SHVSALMSGVMTKVAVYGFIRVVFDLLGTPDWSTSGIVLALGGITAALGILYALMEKDLKRLLAYSTIENVGIIFVSLGL
ALAFQANHQPLPAALAFTAALFHVLNHSLFKSLLFFGAGAVLSATGERDMDKLGGLIHRMPVTSVVFLIGSVAISALPPF
NGFVSEWLIFQAVLQSPQLPQWGLKILVPAVGGLMALAAALAAACFVKAYGVTFLGRPRGAATLAAKEVDRFSLAAMAIL
AALCLAAGVLPGLVMDTLAPIATEILGSRLAPQTPLAWLSIVPIAQSHSSYNGLLVALFIAVSASLLFVAIRLFSSGAVR
RGPAWGCGFTEPAPLAQYSAAGFAQPIRRVFGTLIFRARDHVTMPPPGDTAPARLRIELHDQIWERIYAPLAGGVMAASV
RLNRMQLLTIRGYLSLVFATLVTLLLVLAIWT

Sequences:

>Translated_672_residues
MTELAVQLWCVAVLLAIAVIAVPLSRRPGLATATIYSATLVTCAVATASALTWLLLHADAAAQRTLPIGLPWLGAHLRLD
ALAAFFLVVVNLGGTLASLYALGYGRHEHAPHRVLPFYPAFLAGMNMVVLAADAFSYLLSWEFMSLASWALVMAHHRDGS
NRRAGYVYLLMASFGTLALLLAFGLLAGAAGNYDFAAIRAAQHAPWIATLVLILLLLGAGSKAGLVPLHVWLPLAHPAAP
SHVSALMSGVMTKVAVYGFIRVVFDLLGTPDWSTSGIVLALGGITAALGILYALMEKDLKRLLAYSTIENVGIIFVSLGL
ALAFQANHQPLPAALAFTAALFHVLNHSLFKSLLFFGAGAVLSATGERDMDKLGGLIHRMPVTSVVFLIGSVAISALPPF
NGFVSEWLIFQAVLQSPQLPQWGLKILVPAVGGLMALAAALAAACFVKAYGVTFLGRPRGAATLAAKEVDRFSLAAMAIL
AALCLAAGVLPGLVMDTLAPIATEILGSRLAPQTPLAWLSIVPIAQSHSSYNGLLVALFIAVSASLLFVAIRLFSSGAVR
RGPAWGCGFTEPAPLAQYSAAGFAQPIRRVFGTLIFRARDHVTMPPPGDTAPARLRIELHDQIWERIYAPLAGGVMAASV
RLNRMQLLTIRGYLSLVFATLVTLLLVLAIWT
>Mature_671_residues
TELAVQLWCVAVLLAIAVIAVPLSRRPGLATATIYSATLVTCAVATASALTWLLLHADAAAQRTLPIGLPWLGAHLRLDA
LAAFFLVVVNLGGTLASLYALGYGRHEHAPHRVLPFYPAFLAGMNMVVLAADAFSYLLSWEFMSLASWALVMAHHRDGSN
RRAGYVYLLMASFGTLALLLAFGLLAGAAGNYDFAAIRAAQHAPWIATLVLILLLLGAGSKAGLVPLHVWLPLAHPAAPS
HVSALMSGVMTKVAVYGFIRVVFDLLGTPDWSTSGIVLALGGITAALGILYALMEKDLKRLLAYSTIENVGIIFVSLGLA
LAFQANHQPLPAALAFTAALFHVLNHSLFKSLLFFGAGAVLSATGERDMDKLGGLIHRMPVTSVVFLIGSVAISALPPFN
GFVSEWLIFQAVLQSPQLPQWGLKILVPAVGGLMALAAALAAACFVKAYGVTFLGRPRGAATLAAKEVDRFSLAAMAILA
ALCLAAGVLPGLVMDTLAPIATEILGSRLAPQTPLAWLSIVPIAQSHSSYNGLLVALFIAVSASLLFVAIRLFSSGAVRR
GPAWGCGFTEPAPLAQYSAAGFAQPIRRVFGTLIFRARDHVTMPPPGDTAPARLRIELHDQIWERIYAPLAGGVMAASVR
LNRMQLLTIRGYLSLVFATLVTLLLVLAIWT

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=435, Percent_Identity=26.4367816091954, Blast_Score=109, Evalue=9e-24,
Organism=Escherichia coli, GI1788827, Length=607, Percent_Identity=38.7149917627677, Blast_Score=352, Evalue=4e-98,
Organism=Escherichia coli, GI2367154, Length=520, Percent_Identity=32.5, Blast_Score=222, Evalue=8e-59,
Organism=Escherichia coli, GI1788831, Length=360, Percent_Identity=33.0555555555556, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI1788829, Length=397, Percent_Identity=31.7380352644836, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788614, Length=346, Percent_Identity=33.2369942196532, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788613, Length=369, Percent_Identity=25.4742547425474, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI145693160, Length=290, Percent_Identity=25.8620689655172, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 71068; Mature: 70937

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELAVQLWCVAVLLAIAVIAVPLSRRPGLATATIYSATLVTCAVATASALTWLLLHADA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AAQRTLPIGLPWLGAHLRLDALAAFFLVVVNLGGTLASLYALGYGRHEHAPHRVLPFYPA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHH
FLAGMNMVVLAADAFSYLLSWEFMSLASWALVMAHHRDGSNRRAGYVYLLMASFGTLALL
HHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHH
LAFGLLAGAAGNYDFAAIRAAQHAPWIATLVLILLLLGAGSKAGLVPLHVWLPLAHPAAP
HHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCH
SHVSALMSGVMTKVAVYGFIRVVFDLLGTPDWSTSGIVLALGGITAALGILYALMEKDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RLLAYSTIENVGIIFVSLGLALAFQANHQPLPAALAFTAALFHVLNHSLFKSLLFFGAGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VLSATGERDMDKLGGLIHRMPVTSVVFLIGSVAISALPPFNGFVSEWLIFQAVLQSPQLP
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
QWGLKILVPAVGGLMALAAALAAACFVKAYGVTFLGRPRGAATLAAKEVDRFSLAAMAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHH
AALCLAAGVLPGLVMDTLAPIATEILGSRLAPQTPLAWLSIVPIAQSHSSYNGLLVALFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
AVSASLLFVAIRLFSSGAVRRGPAWGCGFTEPAPLAQYSAAGFAQPIRRVFGTLIFRARD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HVTMPPPGDTAPARLRIELHDQIWERIYAPLAGGVMAASVRLNRMQLLTIRGYLSLVFAT
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTLLLVLAIWT
HHHHHHHHHHCC
>Mature Secondary Structure 
TELAVQLWCVAVLLAIAVIAVPLSRRPGLATATIYSATLVTCAVATASALTWLLLHADA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AAQRTLPIGLPWLGAHLRLDALAAFFLVVVNLGGTLASLYALGYGRHEHAPHRVLPFYPA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHH
FLAGMNMVVLAADAFSYLLSWEFMSLASWALVMAHHRDGSNRRAGYVYLLMASFGTLALL
HHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHH
LAFGLLAGAAGNYDFAAIRAAQHAPWIATLVLILLLLGAGSKAGLVPLHVWLPLAHPAAP
HHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCH
SHVSALMSGVMTKVAVYGFIRVVFDLLGTPDWSTSGIVLALGGITAALGILYALMEKDLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RLLAYSTIENVGIIFVSLGLALAFQANHQPLPAALAFTAALFHVLNHSLFKSLLFFGAGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VLSATGERDMDKLGGLIHRMPVTSVVFLIGSVAISALPPFNGFVSEWLIFQAVLQSPQLP
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
QWGLKILVPAVGGLMALAAALAAACFVKAYGVTFLGRPRGAATLAAKEVDRFSLAAMAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHH
AALCLAAGVLPGLVMDTLAPIATEILGSRLAPQTPLAWLSIVPIAQSHSSYNGLLVALFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
AVSASLLFVAIRLFSSGAVRRGPAWGCGFTEPAPLAQYSAAGFAQPIRRVFGTLIFRARD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
HVTMPPPGDTAPARLRIELHDQIWERIYAPLAGGVMAASVRLNRMQLLTIRGYLSLVFAT
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTLLLVLAIWT
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]