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The map label for this gene is hycD [H]

Identifier: 86748386

GI number: 86748386

Start: 1443793

End: 1444743

Strand: Direct

Name: hycD [H]

Synonym: RPB_1261

Alternate gene names: 86748386

Gene position: 1443793-1444743 (Clockwise)

Preceding gene: 86748385

Following gene: 86748387

Centisome position: 27.08

GC content: 65.51

Gene sequence:

>951_bases
GTGATCACCGACATCGCCGTGCAGGGCGTGCAGATGCTGCTGGTGTTCCTGCTGGCGCCGCTGCTCACCGGCTTCATCCG
CAAGGTCAAGGCGCGGCTGCAGCGCCGCCGCGGCCCGTCGCTGCTGCAGCCCTATCGCGACCTGATCCGGCTGTTGCGCA
AGGAGGTCGTGCTCGCCGACAACGCCTCCTGGCTGTTCCGCGCCACGCCCTACGTCACTTTCGCGGCGATCTGGGTCGCC
GCCGCTTTGGTGCCGACCTTCGCCACCGGGCTGTTGTTCAACTGGACAGCGGATCTGATCGTCATCGTCGCACTGCTCGG
CACCGCGCGGTTCTTCCTCGCGCTCGCCGGCATGGACGTCGGCACCTCGTTCGGCGGCATCGGCTCGTCGCGCGAGGTGA
TGATCGCAGCCCTTGCCGAGCCGGCGATGCTGATGATCGTGTTCTGCGTCGCGCTGGTCGCGGGCACCACGCATCTGTCG
ACCGTCGCCAATTACATCGCGTCGACCGCGGTCGGCCTGCGGGTTTCGCTCGGGCTGGCGATGATCGCGCTGGTGATGGT
GGCGCTCGCCGAGAACGCCCGCATCCCGATCGACAATCCCTCGACCCATCTCGAACTCACCATGGTGCACGAGGCGATGA
TCCTGGAATATTCCGGCCGGCATCTGGCGATGATCGAGTATGGCGCGCAGCTCAAGCTGCTGCTCTATGTCTCGCTGATC
GTCTGCCTGTTCTATCCCTGGGGCATCGGCGTGTTCGGCGCTGGTCCCGCGGCCTATCTGGCCGGCGCGGCGCTGTATCT
GTTCAAGATCACCATTGCGGGCTTTTTGCTGGCGGTGTTCGAGACCGCCACCGCCAAGATGCGGGTGTTCCGGGTGCCGC
AATTCCTCGGTGCGGCCTTGATGCTCGGCCTGCTCGGCACGCTTCTCCTGTTCGTGTCGAAGGGATTGTAA

Upstream 100 bases:

>100_bases
CGGTGCGCCTCAACCGGATGCAGCTCCTCACCATCCGCGGCTATCTCAGCCTGGTGTTCGCCACCCTCGTCACGCTGCTG
CTGGTGCTCGCGATATGGAC

Downstream 100 bases:

>100_bases
GCGATGCACGGGCTCGCTTTCGACATTTCACATTCGCTGGCCGGCGGCCTGGTGCTGGTGTCGTTCATGATGCTGTATCA
GGACCGGTTGACCTCGCTGC

Product: respiratory-chain NADH dehydrogenase, subunit 1

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MITDIAVQGVQMLLVFLLAPLLTGFIRKVKARLQRRRGPSLLQPYRDLIRLLRKEVVLADNASWLFRATPYVTFAAIWVA
AALVPTFATGLLFNWTADLIVIVALLGTARFFLALAGMDVGTSFGGIGSSREVMIAALAEPAMLMIVFCVALVAGTTHLS
TVANYIASTAVGLRVSLGLAMIALVMVALAENARIPIDNPSTHLELTMVHEAMILEYSGRHLAMIEYGAQLKLLLYVSLI
VCLFYPWGIGVFGAGPAAYLAGAALYLFKITIAGFLLAVFETATAKMRVFRVPQFLGAALMLGLLGTLLLFVSKGL

Sequences:

>Translated_316_residues
MITDIAVQGVQMLLVFLLAPLLTGFIRKVKARLQRRRGPSLLQPYRDLIRLLRKEVVLADNASWLFRATPYVTFAAIWVA
AALVPTFATGLLFNWTADLIVIVALLGTARFFLALAGMDVGTSFGGIGSSREVMIAALAEPAMLMIVFCVALVAGTTHLS
TVANYIASTAVGLRVSLGLAMIALVMVALAENARIPIDNPSTHLELTMVHEAMILEYSGRHLAMIEYGAQLKLLLYVSLI
VCLFYPWGIGVFGAGPAAYLAGAALYLFKITIAGFLLAVFETATAKMRVFRVPQFLGAALMLGLLGTLLLFVSKGL
>Mature_316_residues
MITDIAVQGVQMLLVFLLAPLLTGFIRKVKARLQRRRGPSLLQPYRDLIRLLRKEVVLADNASWLFRATPYVTFAAIWVA
AALVPTFATGLLFNWTADLIVIVALLGTARFFLALAGMDVGTSFGGIGSSREVMIAALAEPAMLMIVFCVALVAGTTHLS
TVANYIASTAVGLRVSLGLAMIALVMVALAENARIPIDNPSTHLELTMVHEAMILEYSGRHLAMIEYGAQLKLLLYVSLI
VCLFYPWGIGVFGAGPAAYLAGAALYLFKITIAGFLLAVFETATAKMRVFRVPQFLGAALMLGLLGTLLLFVSKGL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=286, Percent_Identity=34.6153846153846, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082115, Length=240, Percent_Identity=36.25, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 34022; Mature: 34022

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDIAVQGVQMLLVFLLAPLLTGFIRKVKARLQRRRGPSLLQPYRDLIRLLRKEVVLAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
NASWLFRATPYVTFAAIWVAAALVPTFATGLLFNWTADLIVIVALLGTARFFLALAGMDV
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTSFGGIGSSREVMIAALAEPAMLMIVFCVALVAGTTHLSTVANYIASTAVGLRVSLGLA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MIALVMVALAENARIPIDNPSTHLELTMVHEAMILEYSGRHLAMIEYGAQLKLLLYVSLI
HHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
VCLFYPWGIGVFGAGPAAYLAGAALYLFKITIAGFLLAVFETATAKMRVFRVPQFLGAAL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGLLGTLLLFVSKGL
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITDIAVQGVQMLLVFLLAPLLTGFIRKVKARLQRRRGPSLLQPYRDLIRLLRKEVVLAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
NASWLFRATPYVTFAAIWVAAALVPTFATGLLFNWTADLIVIVALLGTARFFLALAGMDV
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTSFGGIGSSREVMIAALAEPAMLMIVFCVALVAGTTHLSTVANYIASTAVGLRVSLGLA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MIALVMVALAENARIPIDNPSTHLELTMVHEAMILEYSGRHLAMIEYGAQLKLLLYVSLI
HHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
VCLFYPWGIGVFGAGPAAYLAGAALYLFKITIAGFLLAVFETATAKMRVFRVPQFLGAAL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLGLLGTLLLFVSKGL
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]