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The map label for this gene is usf [H]

Identifier: 86748327

GI number: 86748327

Start: 1377095

End: 1377769

Strand: Direct

Name: usf [H]

Synonym: RPB_1202

Alternate gene names: 86748327

Gene position: 1377095-1377769 (Clockwise)

Preceding gene: 86748326

Following gene: 86748328

Centisome position: 25.83

GC content: 63.41

Gene sequence:

>675_bases
ATGGGCACGATCATCACCTTCAAGCGTCCCGACGGTCAGGATGCCAGCGGCTATCTCGCCAATGCCGCGCGCGGCAACGC
CCCGGGCGTCGTCGTGATTCAGGAATGGTGGGGCCTGTCCGAACAGATCAAGGGACTGACTGATCGTTTCGCGCTCGCCG
GCTTCGATGCGCTGGCGCCGGATCTCTACAACGGCAAGGTGGTGCCCTATCACGACACGGACGCCGCTAACAAAGAGATG
AGCTCACTCGATTTCATGGACGCGACCACACAGACCGTGCGCGGCGCGGCCGGCTATCTGGCACGCAACGGCGCCAAGGT
AGGTCTCACCGGATTCTGTCTCGGCGGCGCCGTCACGGTGATCGGCGCGTGCAAAATCCCCGAACTGAGTGCAGCGGTTG
TGTTCTACGGCATCCCCCCGGAACAGGCGGCGAAACCGTCCGACGTCCGCGTGCCGATGCAGGGCCACTTCGCCAATCGC
GACGATTGGTGCACGCCGCAAGTGGTCGACGCGTTCGAGAAAGGGCTGAAAGAGGCGGGAAAGAGCGCGGAATTCTTCCG
TTACGATGCCGATCACGCCTTCGTCAACGAACAGCGCGCCGCAGTGCACGATCGAGAGGCCGCGGAACTCGCGTGGTCGC
GCGCCACGGCATTCTTTGGAAAGCATCTCGGCTGA

Upstream 100 bases:

>100_bases
ATGCGGAGTCCGCGATCTGCCGTGGCGACTCCGGCGATTGCATCTTTCAATCCCCGCGGACACAATGTGCTCAACGAAGT
GCAACTCACGTGGAGGAAAC

Downstream 100 bases:

>100_bases
TTGGCCGCTGCCGCTAAAATTTGAGCATTCGTCTGAAGGGTTGGAAGAAGAGTTTGCTGCCATAAGCCTGTCACATCTAC
GAGGACAGCAATGGTTGAGA

Product: carboxymethylenebutenolidase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGTIITFKRPDGQDASGYLANAARGNAPGVVVIQEWWGLSEQIKGLTDRFALAGFDALAPDLYNGKVVPYHDTDAANKEM
SSLDFMDATTQTVRGAAGYLARNGAKVGLTGFCLGGAVTVIGACKIPELSAAVVFYGIPPEQAAKPSDVRVPMQGHFANR
DDWCTPQVVDAFEKGLKEAGKSAEFFRYDADHAFVNEQRAAVHDREAAELAWSRATAFFGKHLG

Sequences:

>Translated_224_residues
MGTIITFKRPDGQDASGYLANAARGNAPGVVVIQEWWGLSEQIKGLTDRFALAGFDALAPDLYNGKVVPYHDTDAANKEM
SSLDFMDATTQTVRGAAGYLARNGAKVGLTGFCLGGAVTVIGACKIPELSAAVVFYGIPPEQAAKPSDVRVPMQGHFANR
DDWCTPQVVDAFEKGLKEAGKSAEFFRYDADHAFVNEQRAAVHDREAAELAWSRATAFFGKHLG
>Mature_223_residues
GTIITFKRPDGQDASGYLANAARGNAPGVVVIQEWWGLSEQIKGLTDRFALAGFDALAPDLYNGKVVPYHDTDAANKEMS
SLDFMDATTQTVRGAAGYLARNGAKVGLTGFCLGGAVTVIGACKIPELSAAVVFYGIPPEQAAKPSDVRVPMQGHFANRD
DWCTPQVVDAFEKGLKEAGKSAEFFRYDADHAFVNEQRAAVHDREAAELAWSRATAFFGKHLG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=216, Percent_Identity=31.0185185185185, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 24030; Mature: 23899

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTIITFKRPDGQDASGYLANAARGNAPGVVVIQEWWGLSEQIKGLTDRFALAGFDALAP
CCCEEEEECCCCCCCCCEEECCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCHHHHCC
DLYNGKVVPYHDTDAANKEMSSLDFMDATTQTVRGAAGYLARNGAKVGLTGFCLGGAVTV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEHHHHEEE
IGACKIPELSAAVVFYGIPPEQAAKPSDVRVPMQGHFANRDDWCTPQVVDAFEKGLKEAG
HCCCCCCCCCEEEEEEECCHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
KSAEFFRYDADHAFVNEQRAAVHDREAAELAWSRATAFFGKHLG
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GTIITFKRPDGQDASGYLANAARGNAPGVVVIQEWWGLSEQIKGLTDRFALAGFDALAP
CCEEEEECCCCCCCCCEEECCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCHHHHCC
DLYNGKVVPYHDTDAANKEMSSLDFMDATTQTVRGAAGYLARNGAKVGLTGFCLGGAVTV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEHHHHEEE
IGACKIPELSAAVVFYGIPPEQAAKPSDVRVPMQGHFANRDDWCTPQVVDAFEKGLKEAG
HCCCCCCCCCEEEEEEECCHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
KSAEFFRYDADHAFVNEQRAAVHDREAAELAWSRATAFFGKHLG
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]