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The map label for this gene is trmL [H]

Identifier: 86748324

GI number: 86748324

Start: 1373559

End: 1374011

Strand: Reverse

Name: trmL [H]

Synonym: RPB_1199

Alternate gene names: 86748324

Gene position: 1374011-1373559 (Counterclockwise)

Preceding gene: 86748329

Following gene: 86748323

Centisome position: 25.77

GC content: 59.6

Gene sequence:

>453_bases
ATGCGGATCGCCCTGTACCAGCCCGACATTCCGCAGAATACCGGGACTCTGCTTCGGTTTTGCGCCTGTTTGAACGTCGA
AGCTCACATCATCGAGCCCGCCGGTTTCCCGACATCGGATCGGCATTTCCGTCGTGCGGGAATGGACTATCTGGATCAGA
TCACCATCGTCCGGCACGATTCCTGGTCAAAATTCGAGCAATGGAGGAAAGACGCGGACTGCCGCGTGATTTTGCTTACA
ACGAAAGGCGTAACGCCCTATCTCGAACAGCGTTATCAATCTACTGACGTGCTGCTACTGGGCCGGGAAAGCGCTGGCGT
GCCGGACGAAGTCGCCGCCGCGGCCGACGCTCGCATCGTGATTCCAATGCAGCCGGGGTTTCGCTCGCTCAACGTCGCGA
TGGCCGCGGCGCTGGTTGTCGGCGAAGCGCTTCGCCAGACCCGACAAGGTTAG

Upstream 100 bases:

>100_bases
GCGCAAGCCCGCGAAGGGCTAATCAGCACGGCCCGAACGGTTCGGACGCAATGTCGTGCACACTGCGAGTTATGATTCAG
TTGCTTGGGAGCATTACGGA

Downstream 100 bases:

>100_bases
AGGATACGACGCGGTGAGTTATGCGGTCAAAGAGATCTTCCTCACCCTGCAGGGCGAAGGCGCGCATGCCGGTCGCGCAT
CGGTGTTTTGCAGGTTCGCG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MRIALYQPDIPQNTGTLLRFCACLNVEAHIIEPAGFPTSDRHFRRAGMDYLDQITIVRHDSWSKFEQWRKDADCRVILLT
TKGVTPYLEQRYQSTDVLLLGRESAGVPDEVAAAADARIVIPMQPGFRSLNVAMAAALVVGEALRQTRQG

Sequences:

>Translated_150_residues
MRIALYQPDIPQNTGTLLRFCACLNVEAHIIEPAGFPTSDRHFRRAGMDYLDQITIVRHDSWSKFEQWRKDADCRVILLT
TKGVTPYLEQRYQSTDVLLLGRESAGVPDEVAAAADARIVIPMQPGFRSLNVAMAAALVVGEALRQTRQG
>Mature_150_residues
MRIALYQPDIPQNTGTLLRFCACLNVEAHIIEPAGFPTSDRHFRRAGMDYLDQITIVRHDSWSKFEQWRKDADCRVILLT
TKGVTPYLEQRYQSTDVLLLGRESAGVPDEVAAAADARIVIPMQPGFRSLNVAMAAALVVGEALRQTRQG

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=40.5405405405405, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16770; Mature: 16770

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALYQPDIPQNTGTLLRFCACLNVEAHIIEPAGFPTSDRHFRRAGMDYLDQITIVRHD
CEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCHHHHHHEEEEECC
SWSKFEQWRKDADCRVILLTTKGVTPYLEQRYQSTDVLLLGRESAGVPDEVAAAADARIV
CHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCEEE
IPMQPGFRSLNVAMAAALVVGEALRQTRQG
EECCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIALYQPDIPQNTGTLLRFCACLNVEAHIIEPAGFPTSDRHFRRAGMDYLDQITIVRHD
CEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCHHHHHHEEEEECC
SWSKFEQWRKDADCRVILLTTKGVTPYLEQRYQSTDVLLLGRESAGVPDEVAAAADARIV
CHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCEEE
IPMQPGFRSLNVAMAAALVVGEALRQTRQG
EECCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA