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The map label for this gene is purU [H]

Identifier: 86748270

GI number: 86748270

Start: 1316953

End: 1317816

Strand: Direct

Name: purU [H]

Synonym: RPB_1145

Alternate gene names: 86748270

Gene position: 1316953-1317816 (Clockwise)

Preceding gene: 86748269

Following gene: 86748272

Centisome position: 24.7

GC content: 64.12

Gene sequence:

>864_bases
ATGCCTCACACCAATGTCGTGCTGTCGCTGTCCTGCCGCGACCAGCCGGGAATCGTCGCAGCGGTCTCCAAGCTGCTGTT
CGACGCCGGCTGCAATGTCGTCGAGGCGCATCAGTTCAACGATGCCGAGACCCATCTGTTCTTCATGCGGATGCGCTTCG
CGATGCTGAAGCCGGACGCCAGCATTGCGGCGCTCGAGGCCGCCTTCGAGCCGGTCGCGAACGAATTCGGCATCACCTGG
ACAATGCGCAGCCTCAGCGAGAAGCAGCGGGTGATGATCCTGGTGTCGAAGTTCGACCATTGCCTCGCCGACCTGCTGTA
TCGCTGGCGCACCGGCGAACTTGCGATGGATGTCGCCGGCATCATCGCCAACCATCCGCGCGAGACCTACGCGCATCTCG
ATCTCGACGGCATCCCGTTCCACTATCTGCCGGTCACCAAGCCGACCAAGATGGAGCAGGAGGCGCAGGTCTGGGAGCTG
ATCCGCGCCGCCAATACCGACCTCGTCGTGCTGGCGCGCTACATGCAGGTGCTGTCGGACGGGCTGTGCGCGAAGCTCGC
CGGCCGCTGCATCAACATCCACCACTCGTTCCTGCCGGGCTTCAAGGGCGCGCGGCCGTATCACCAGGCGTTCGAACGCG
GCGTCAAGCTGATCGGCGCCACCGCGCATTACGTCACGCCCGATCTCGACGAAGGCCCGATCATCGAGCAGGACGTCGAG
CGCATCAGCCATCACAATTGCGTCGAGGATCTGGTGCGCAAGGGCCGCGAGATCGAACGCCGCGTGCTCGCCCGCGCCAT
CACCTGGCACATCGACGGCCGGGTGATCGTCAACGGCACGAAGACGGTGGTGTTCAAGGATTGA

Upstream 100 bases:

>100_bases
CGCCGGCGGCCTCGCGGTGGCGCCCGATCGAGGTTGAAGCCTCTCGCCCGGTACTGACTTTCACGCGGATTTTCCGCGCG
CTTTCGCATGGAGTCTTTCG

Downstream 100 bases:

>100_bases
GGAAACTCGGACTCGTCATCCTGAGGTGCGCGCCCTTGCGCGCTTCTGAATCGTCATCCTGAGGTGCGAGCGTAGCGAGC
CTCGAAGGATTCGGCGACGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPHTNVVLSLSCRDQPGIVAAVSKLLFDAGCNVVEAHQFNDAETHLFFMRMRFAMLKPDASIAALEAAFEPVANEFGITW
TMRSLSEKQRVMILVSKFDHCLADLLYRWRTGELAMDVAGIIANHPRETYAHLDLDGIPFHYLPVTKPTKMEQEAQVWEL
IRAANTDLVVLARYMQVLSDGLCAKLAGRCINIHHSFLPGFKGARPYHQAFERGVKLIGATAHYVTPDLDEGPIIEQDVE
RISHHNCVEDLVRKGREIERRVLARAITWHIDGRVIVNGTKTVVFKD

Sequences:

>Translated_287_residues
MPHTNVVLSLSCRDQPGIVAAVSKLLFDAGCNVVEAHQFNDAETHLFFMRMRFAMLKPDASIAALEAAFEPVANEFGITW
TMRSLSEKQRVMILVSKFDHCLADLLYRWRTGELAMDVAGIIANHPRETYAHLDLDGIPFHYLPVTKPTKMEQEAQVWEL
IRAANTDLVVLARYMQVLSDGLCAKLAGRCINIHHSFLPGFKGARPYHQAFERGVKLIGATAHYVTPDLDEGPIIEQDVE
RISHHNCVEDLVRKGREIERRVLARAITWHIDGRVIVNGTKTVVFKD
>Mature_286_residues
PHTNVVLSLSCRDQPGIVAAVSKLLFDAGCNVVEAHQFNDAETHLFFMRMRFAMLKPDASIAALEAAFEPVANEFGITWT
MRSLSEKQRVMILVSKFDHCLADLLYRWRTGELAMDVAGIIANHPRETYAHLDLDGIPFHYLPVTKPTKMEQEAQVWELI
RAANTDLVVLARYMQVLSDGLCAKLAGRCINIHHSFLPGFKGARPYHQAFERGVKLIGATAHYVTPDLDEGPIIEQDVER
ISHHNCVEDLVRKGREIERRVLARAITWHIDGRVIVNGTKTVVFKD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=29.0155440414508, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=29.0155440414508, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=29.0155440414508, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787483, Length=284, Percent_Identity=40.4929577464789, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1788846, Length=109, Percent_Identity=35.7798165137615, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=29.8913043478261, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24582400, Length=214, Percent_Identity=28.9719626168224, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32366; Mature: 32235

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHTNVVLSLSCRDQPGIVAAVSKLLFDAGCNVVEAHQFNDAETHLFFMRMRFAMLKPDA
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
SIAALEAAFEPVANEFGITWTMRSLSEKQRVMILVSKFDHCLADLLYRWRTGELAMDVAG
HHHHHHHHHHHHHHHCCCEEEHHHHCCHHHEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IIANHPRETYAHLDLDGIPFHYLPVTKPTKMEQEAQVWELIRAANTDLVVLARYMQVLSD
HHHCCCCHHEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
GLCAKLAGRCINIHHSFLPGFKGARPYHQAFERGVKLIGATAHYVTPDLDEGPIIEQDVE
CHHHHHHHHHEEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHH
RISHHNCVEDLVRKGREIERRVLARAITWHIDGRVIVNGTKTVVFKD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCEEEEECCEEEEEEC
>Mature Secondary Structure 
PHTNVVLSLSCRDQPGIVAAVSKLLFDAGCNVVEAHQFNDAETHLFFMRMRFAMLKPDA
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
SIAALEAAFEPVANEFGITWTMRSLSEKQRVMILVSKFDHCLADLLYRWRTGELAMDVAG
HHHHHHHHHHHHHHHCCCEEEHHHHCCHHHEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IIANHPRETYAHLDLDGIPFHYLPVTKPTKMEQEAQVWELIRAANTDLVVLARYMQVLSD
HHHCCCCHHEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
GLCAKLAGRCINIHHSFLPGFKGARPYHQAFERGVKLIGATAHYVTPDLDEGPIIEQDVE
CHHHHHHHHHEEHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHH
RISHHNCVEDLVRKGREIERRVLARAITWHIDGRVIVNGTKTVVFKD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]