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The map label for this gene is aglA [H]

Identifier: 86747936

GI number: 86747936

Start: 925461

End: 927083

Strand: Direct

Name: aglA [H]

Synonym: RPB_0810

Alternate gene names: 86747936

Gene position: 925461-927083 (Clockwise)

Preceding gene: 86747933

Following gene: 86747940

Centisome position: 17.36

GC content: 67.53

Gene sequence:

>1623_bases
ATGCCGATGACCAAAGAGACGAGCCCGTCCTGGTGGGCGGCCGGTGCGCTGTATCAGATCTATCCCCGCTCGTTTCAGGA
CTCCGATTCCGACGGCATCGGCGATCTGCGCGGCATCATCGATCGGCTGGATCATTTGTCGGATCTCGGCGTCGACGCGC
TGTGGCTGTCGCCGATCTTTCCGTCGCCGATGGCGGATTTCGGCTACGACGTCGCCGACTATGTCGGGATCGATCCGATC
TTCGGCACCATGGATGATTTCGACGCGCTGGTGCTGACCGCGCATGCGCGCGGACTCAAGGTGATTCTCGACCTGGTGCC
GAACCATTCGTCCGACCAGCACCCGTGGTTCGTCGAAAGCCGGTCCTCGCGCGACAATCCGAAACGCGACTGGTATCTGT
GGCGAGATCCCGCGCCGGACGGCGGACCGCCGACCAACTGGCTGTCGGAATTCGGCGGCAGCGGCTGGGAATACGACGAT
GCCACCGGCCAGTATTATTATCACGCCTTCCTCAAGCAGCAGCCCGACCTCAACTGGCGCAATCCGCAGGTGCGCGCAGC
GATCTACGATGCGATGCGGTTCTGGCTGAAGAAGGGCGTCGACGGCTTCCGCGTCGACGTGATCTGGCACCTGATCAAGG
ACGATCGATATCGCGACAACCCGCCCAATCCGGAGTTCAGGCCCGGCCTGCCGCCGCACGCCGCCTTGCTGACGGCGTAT
TCGGCCGATCGGCCGGAGACGCAGGAGATCGTCGCGCAGCTGCGCGCGGTGTGCGACGAGTTCGATCAGCGCCTGCTGAT
CGGCGAGATCTATCTGCCGATCGAGCGGCTGGTGGCGTATTACGGCGCCGATCTGCGCGGCGCGCATCTGCCGTTCAACT
TCGCGCTGCTGTCGACGCCGTGGCGCGCGCACGACATCGCGGCGCTGATCGATCGCTACGAGGCGGCGCTGCCGCCGGGC
GCGTGGCCGAACTGGGTGCTCGGCAATCACGACCGGCCGCGGGTGGCGAGCCGCGTCGGTCCTGCGCAGGCCCGCGTTGC
CGCGATGCTGCTGCTGACGCTGCGCGGCACGCCGACGCTGTATTACGGCGACGAGCTCGGCATGGAGCAGGTCGACATCG
CGCCGCAGGACGTGCAGGACCCGTTCGAGAAGAACGTGCCGGGCATCGGGGTCGGCCGCGACGGCTGCCGCACGCCGATG
CCGTGGGATTCCTCGCCGAACGCCGGCTTTTCCGACGCGAAGCCATGGCTGCCGCTGGGGCCCGACGCGGCGCAGGACAA
CGTCGCCAATCTGCGCGCCGACGCGCAGTCGATCCTCAATCTGTATCGCGCGCTGCTGCGGCTGCGCCGCGCCCGGCCGC
AATTGTCGCTCGGTGATTATCAGCCGCTCGCGGTGCAGGGCGAGCTGCTGCTGTATCGCCGCTGTCATCAGGGCGAATCG
GTGCTGATCGCACTCAATCTCGGCGCCGCGCCGGTGTCGGCGGCGTCCGATGCGTTCGGGCTCGACGGCGAGGTGCTGCT
GTCGACCCTCATGGACCGCGCCGGCGAACGCGTCGGCGCCACGCTCGATCTGCGCGGCCACGAGGGCGTCATCATCGGCC
GCGCGCCCGAGCCGGTGGTGTAG

Upstream 100 bases:

>100_bases
GAGCTTCACGGCACTGACGCACCGTTGTGCGATGTGGCGACACGCGACGCCCCGGAACCACGCGACGGGTTCGGGCGTCT
GTGACGGGGCAGAACAAGGA

Downstream 100 bases:

>100_bases
TCGCGGTGAAGCGATGAGGCCGCTGCTCCCCTCCCCCTTGCGGGGAGGGGTCGGGGGGTGGGGGTCCCCGAGCACTGCGC
ACGCGATTCCGGTACTTCGT

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MPMTKETSPSWWAAGALYQIYPRSFQDSDSDGIGDLRGIIDRLDHLSDLGVDALWLSPIFPSPMADFGYDVADYVGIDPI
FGTMDDFDALVLTAHARGLKVILDLVPNHSSDQHPWFVESRSSRDNPKRDWYLWRDPAPDGGPPTNWLSEFGGSGWEYDD
ATGQYYYHAFLKQQPDLNWRNPQVRAAIYDAMRFWLKKGVDGFRVDVIWHLIKDDRYRDNPPNPEFRPGLPPHAALLTAY
SADRPETQEIVAQLRAVCDEFDQRLLIGEIYLPIERLVAYYGADLRGAHLPFNFALLSTPWRAHDIAALIDRYEAALPPG
AWPNWVLGNHDRPRVASRVGPAQARVAAMLLLTLRGTPTLYYGDELGMEQVDIAPQDVQDPFEKNVPGIGVGRDGCRTPM
PWDSSPNAGFSDAKPWLPLGPDAAQDNVANLRADAQSILNLYRALLRLRRARPQLSLGDYQPLAVQGELLLYRRCHQGES
VLIALNLGAAPVSAASDAFGLDGEVLLSTLMDRAGERVGATLDLRGHEGVIIGRAPEPVV

Sequences:

>Translated_540_residues
MPMTKETSPSWWAAGALYQIYPRSFQDSDSDGIGDLRGIIDRLDHLSDLGVDALWLSPIFPSPMADFGYDVADYVGIDPI
FGTMDDFDALVLTAHARGLKVILDLVPNHSSDQHPWFVESRSSRDNPKRDWYLWRDPAPDGGPPTNWLSEFGGSGWEYDD
ATGQYYYHAFLKQQPDLNWRNPQVRAAIYDAMRFWLKKGVDGFRVDVIWHLIKDDRYRDNPPNPEFRPGLPPHAALLTAY
SADRPETQEIVAQLRAVCDEFDQRLLIGEIYLPIERLVAYYGADLRGAHLPFNFALLSTPWRAHDIAALIDRYEAALPPG
AWPNWVLGNHDRPRVASRVGPAQARVAAMLLLTLRGTPTLYYGDELGMEQVDIAPQDVQDPFEKNVPGIGVGRDGCRTPM
PWDSSPNAGFSDAKPWLPLGPDAAQDNVANLRADAQSILNLYRALLRLRRARPQLSLGDYQPLAVQGELLLYRRCHQGES
VLIALNLGAAPVSAASDAFGLDGEVLLSTLMDRAGERVGATLDLRGHEGVIIGRAPEPVV
>Mature_539_residues
PMTKETSPSWWAAGALYQIYPRSFQDSDSDGIGDLRGIIDRLDHLSDLGVDALWLSPIFPSPMADFGYDVADYVGIDPIF
GTMDDFDALVLTAHARGLKVILDLVPNHSSDQHPWFVESRSSRDNPKRDWYLWRDPAPDGGPPTNWLSEFGGSGWEYDDA
TGQYYYHAFLKQQPDLNWRNPQVRAAIYDAMRFWLKKGVDGFRVDVIWHLIKDDRYRDNPPNPEFRPGLPPHAALLTAYS
ADRPETQEIVAQLRAVCDEFDQRLLIGEIYLPIERLVAYYGADLRGAHLPFNFALLSTPWRAHDIAALIDRYEAALPPGA
WPNWVLGNHDRPRVASRVGPAQARVAAMLLLTLRGTPTLYYGDELGMEQVDIAPQDVQDPFEKNVPGIGVGRDGCRTPMP
WDSSPNAGFSDAKPWLPLGPDAAQDNVANLRADAQSILNLYRALLRLRRARPQLSLGDYQPLAVQGELLLYRRCHQGESV
LIALNLGAAPVSAASDAFGLDGEVLLSTLMDRAGERVGATLDLRGHEGVIIGRAPEPVV

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=499, Percent_Identity=37.0741482965932, Blast_Score=341, Evalue=8e-94,
Organism=Homo sapiens, GI65506891, Length=132, Percent_Identity=36.3636363636364, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI61744475, Length=132, Percent_Identity=36.3636363636364, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI61744479, Length=107, Percent_Identity=41.1214953271028, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI61744481, Length=107, Percent_Identity=41.1214953271028, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI61744483, Length=107, Percent_Identity=41.1214953271028, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI61744477, Length=132, Percent_Identity=36.3636363636364, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790687, Length=521, Percent_Identity=37.236084452975, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI1786604, Length=483, Percent_Identity=26.0869565217391, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789995, Length=139, Percent_Identity=30.2158273381295, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565753, Length=501, Percent_Identity=27.7445109780439, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25147709, Length=566, Percent_Identity=25.4416961130742, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6322245, Length=546, Percent_Identity=33.5164835164835, Blast_Score=289, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6321726, Length=541, Percent_Identity=33.641404805915, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322241, Length=544, Percent_Identity=33.2720588235294, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6322021, Length=544, Percent_Identity=33.2720588235294, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324416, Length=544, Percent_Identity=33.8235294117647, Blast_Score=279, Evalue=9e-76,
Organism=Saccharomyces cerevisiae, GI6319776, Length=555, Percent_Identity=32.2522522522522, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6321731, Length=555, Percent_Identity=32.0720720720721, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24586593, Length=535, Percent_Identity=40.9345794392523, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45549022, Length=533, Percent_Identity=39.7748592870544, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24586591, Length=555, Percent_Identity=38.5585585585586, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24583747, Length=500, Percent_Identity=41.4, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24583749, Length=500, Percent_Identity=41.4, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24586589, Length=503, Percent_Identity=42.544731610338, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24583745, Length=551, Percent_Identity=39.5644283121597, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24586599, Length=521, Percent_Identity=39.1554702495201, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24586597, Length=509, Percent_Identity=41.8467583497053, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221330053, Length=552, Percent_Identity=38.0434782608696, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24586587, Length=502, Percent_Identity=40.4382470119522, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI281360393, Length=481, Percent_Identity=38.6694386694387, Blast_Score=308, Evalue=8e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 60092; Mature: 59961

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMTKETSPSWWAAGALYQIYPRSFQDSDSDGIGDLRGIIDRLDHLSDLGVDALWLSPIF
CCCCCCCCCCHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCC
PSPMADFGYDVADYVGIDPIFGTMDDFDALVLTAHARGLKVILDLVPNHSSDQHPWFVES
CCCHHHHCCCHHHHCCCCCCCCCCCCHHHEEEECCCCCCEEHEECCCCCCCCCCCEEEEC
RSSRDNPKRDWYLWRDPAPDGGPPTNWLSEFGGSGWEYDDATGQYYYHAFLKQQPDLNWR
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCCCCC
NPQVRAAIYDAMRFWLKKGVDGFRVDVIWHLIKDDRYRDNPPNPEFRPGLPPHAALLTAY
CCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
SADRPETQEIVAQLRAVCDEFDQRLLIGEIYLPIERLVAYYGADLRGAHLPFNFALLSTP
CCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
WRAHDIAALIDRYEAALPPGAWPNWVLGNHDRPRVASRVGPAQARVAAMLLLTLRGTPTL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEE
YYGDELGMEQVDIAPQDVQDPFEKNVPGIGVGRDGCRTPMPWDSSPNAGFSDAKPWLPLG
EECCCCCCHHCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDAAQDNVANLRADAQSILNLYRALLRLRRARPQLSLGDYQPLAVQGELLLYRRCHQGES
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHCCCCE
VLIALNLGAAPVSAASDAFGLDGEVLLSTLMDRAGERVGATLDLRGHEGVIIGRAPEPVV
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure 
PMTKETSPSWWAAGALYQIYPRSFQDSDSDGIGDLRGIIDRLDHLSDLGVDALWLSPIF
CCCCCCCCCHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCC
PSPMADFGYDVADYVGIDPIFGTMDDFDALVLTAHARGLKVILDLVPNHSSDQHPWFVES
CCCHHHHCCCHHHHCCCCCCCCCCCCHHHEEEECCCCCCEEHEECCCCCCCCCCCEEEEC
RSSRDNPKRDWYLWRDPAPDGGPPTNWLSEFGGSGWEYDDATGQYYYHAFLKQQPDLNWR
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCCCCC
NPQVRAAIYDAMRFWLKKGVDGFRVDVIWHLIKDDRYRDNPPNPEFRPGLPPHAALLTAY
CCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
SADRPETQEIVAQLRAVCDEFDQRLLIGEIYLPIERLVAYYGADLRGAHLPFNFALLSTP
CCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
WRAHDIAALIDRYEAALPPGAWPNWVLGNHDRPRVASRVGPAQARVAAMLLLTLRGTPTL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEE
YYGDELGMEQVDIAPQDVQDPFEKNVPGIGVGRDGCRTPMPWDSSPNAGFSDAKPWLPLG
EECCCCCCHHCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDAAQDNVANLRADAQSILNLYRALLRLRRARPQLSLGDYQPLAVQGELLLYRRCHQGES
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHCCCCE
VLIALNLGAAPVSAASDAFGLDGEVLLSTLMDRAGERVGATLDLRGHEGVIIGRAPEPVV
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]