Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 86747940

GI number: 86747940

Start: 930834

End: 931715

Strand: Direct

Name: gltX [H]

Synonym: RPB_0814

Alternate gene names: 86747940

Gene position: 930834-931715 (Clockwise)

Preceding gene: 86747936

Following gene: 86747941

Centisome position: 17.46

GC content: 69.73

Gene sequence:

>882_bases
ATGCCGCCACCCGTTTTCCGATTCGCCCCGAGCCCGAACGGCTTTCTCCACCTCGGTCACGCTTATTCCGCGTTGCTGAA
TTTCGATTTGGCGCGTGCGAGCGGCGGCCGGCTGCTGCTGCGGATCGAGGACATCGATGCCACGCGCTGCCGGCCCGAGT
ATGAAGCGGCGATCGTCGACGATCTGGCCTGGCTCGGCATCGCCTGGGAGCCGGAGGTGCGGCGGCAATCCGATCACTTG
GATCTGTATCGCGACGCGGTCGAGCGGCTGGCAGCGCGCGGGCTGGTGTATCCGGCGTTCGAGAGTCGCGCCGAGATCGC
CCGCCTGGTCGCGGCGAAGGAGACGACGGCGCCATGGCCGCGTGACCCCGACGGCGCGCCGCTGTATCCGGGCTCGGCCA
AGTTGCTCTCGGCGGAGCAACGCGCGCAACTGATCGGCTCCGGCGCGCCCTATGCGCTGCGGCTCGACATGGAAGCTGCG
CGGGCGCTCGCCGGCGAGTTGTTCTGGCGCGAGCTTAGTCTCGGCCCCGACGGTGAGGCCGGCATGGTCGCGGCCCGGCC
CGAAGCCTGGGGCGACGTGGTGATCGCCCGCAAAGAGACGCCGACCAGCTATCACCTCGCGGTGGTGATCGACGACGCGC
TGCAGGGCGTCACCGAGGTGGTGCGCGGCGCCGACCTGTTCTGGTCGACCAGCGTCCACCGCCTGCTCCAGACTTTGCTC
GATTTGCCGGCTCCGGTATATCGGCACCATCCACTGCTGCGCGATGCCGAGGGGCGCAAGCTGTCGAAGTCGACCGGCGC
CACCGGGCTGCGCGAATTGCGCGCCGCGGGGGGCACGCCGAGCGAGATCCGCGCGATGGTCGGGCTGGCTGCATCTGCCT
GA

Upstream 100 bases:

>100_bases
TCATGCGCGGGCTTGACCCGCGCATCCATCCGTACTCGTAAGACGAGTCTTCCACGCGATGGATGGCCGGATCAAGTCCG
GCAATGACGCCGGGGTTTCG

Downstream 100 bases:

>100_bases
AGTTTGCCCGAACTTTGCCGCAAGCCGGACAGCCCCGAAATCTCGTTTTCCCGATGGCTCCGCCAAGGCCGCCATGGCAT
GGTGCCGGGCGTTGGGGTCG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPPPVFRFAPSPNGFLHLGHAYSALLNFDLARASGGRLLLRIEDIDATRCRPEYEAAIVDDLAWLGIAWEPEVRRQSDHL
DLYRDAVERLAARGLVYPAFESRAEIARLVAAKETTAPWPRDPDGAPLYPGSAKLLSAEQRAQLIGSGAPYALRLDMEAA
RALAGELFWRELSLGPDGEAGMVAARPEAWGDVVIARKETPTSYHLAVVIDDALQGVTEVVRGADLFWSTSVHRLLQTLL
DLPAPVYRHHPLLRDAEGRKLSKSTGATGLRELRAAGGTPSEIRAMVGLAASA

Sequences:

>Translated_293_residues
MPPPVFRFAPSPNGFLHLGHAYSALLNFDLARASGGRLLLRIEDIDATRCRPEYEAAIVDDLAWLGIAWEPEVRRQSDHL
DLYRDAVERLAARGLVYPAFESRAEIARLVAAKETTAPWPRDPDGAPLYPGSAKLLSAEQRAQLIGSGAPYALRLDMEAA
RALAGELFWRELSLGPDGEAGMVAARPEAWGDVVIARKETPTSYHLAVVIDDALQGVTEVVRGADLFWSTSVHRLLQTLL
DLPAPVYRHHPLLRDAEGRKLSKSTGATGLRELRAAGGTPSEIRAMVGLAASA
>Mature_292_residues
PPPVFRFAPSPNGFLHLGHAYSALLNFDLARASGGRLLLRIEDIDATRCRPEYEAAIVDDLAWLGIAWEPEVRRQSDHLD
LYRDAVERLAARGLVYPAFESRAEIARLVAAKETTAPWPRDPDGAPLYPGSAKLLSAEQRAQLIGSGAPYALRLDMEAAR
ALAGELFWRELSLGPDGEAGMVAARPEAWGDVVIARKETPTSYHLAVVIDDALQGVTEVVRGADLFWSTSVHRLLQTLLD
LPAPVYRHHPLLRDAEGRKLSKSTGATGLRELRAAGGTPSEIRAMVGLAASA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=297, Percent_Identity=33.6700336700337, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4826960, Length=157, Percent_Identity=33.7579617834395, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788743, Length=274, Percent_Identity=29.9270072992701, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=34.2105263157895, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786895, Length=92, Percent_Identity=43.4782608695652, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71993394, Length=268, Percent_Identity=28.7313432835821, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324540, Length=285, Percent_Identity=30.8771929824561, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324742, Length=139, Percent_Identity=34.5323741007194, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321192, Length=97, Percent_Identity=35.0515463917526, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=299, Percent_Identity=34.4481605351171, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 31840; Mature: 31708

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPVFRFAPSPNGFLHLGHAYSALLNFDLARASGGRLLLRIEDIDATRCRPEYEAAIVD
CCCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHH
DLAWLGIAWEPEVRRQSDHLDLYRDAVERLAARGLVYPAFESRAEIARLVAAKETTAPWP
HHHHHCEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
RDPDGAPLYPGSAKLLSAEQRAQLIGSGAPYALRLDMEAARALAGELFWRELSLGPDGEA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCC
GMVAARPEAWGDVVIARKETPTSYHLAVVIDDALQGVTEVVRGADLFWSTSVHRLLQTLL
CEEEECCCCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DLPAPVYRHHPLLRDAEGRKLSKSTGATGLRELRAAGGTPSEIRAMVGLAASA
HCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPPVFRFAPSPNGFLHLGHAYSALLNFDLARASGGRLLLRIEDIDATRCRPEYEAAIVD
CCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHH
DLAWLGIAWEPEVRRQSDHLDLYRDAVERLAARGLVYPAFESRAEIARLVAAKETTAPWP
HHHHHCEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
RDPDGAPLYPGSAKLLSAEQRAQLIGSGAPYALRLDMEAARALAGELFWRELSLGPDGEA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCC
GMVAARPEAWGDVVIARKETPTSYHLAVVIDDALQGVTEVVRGADLFWSTSVHRLLQTLL
CEEEECCCCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
DLPAPVYRHHPLLRDAEGRKLSKSTGATGLRELRAAGGTPSEIRAMVGLAASA
HCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]