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The map label for this gene is argF [H]

Identifier: 86747910

GI number: 86747910

Start: 877779

End: 878765

Strand: Direct

Name: argF [H]

Synonym: RPB_0784

Alternate gene names: 86747910

Gene position: 877779-878765 (Clockwise)

Preceding gene: 86747909

Following gene: 86747911

Centisome position: 16.46

GC content: 66.26

Gene sequence:

>987_bases
ATGAGCAGCGCTACCAATCGTGCGGTGAGTAGTTCCAAGAGTACGATCGAGAGTCCAGCGATGACCAGCGCCCCGAGGCA
TTTCCTCGATCTCACCGAGGTCCCGACCTCGGAGCTGCGCAATATGCTCGCCGCCGGCGTGGCGATGAAGGCGAAGCGCA
AGGCCAATGCGGATGCCGACAAGCCGCTCGCCGGCAAGACGCTGGCGATGATCTTCGAGAAGCCCTCGACGCGCACCCGC
GTCTCGTTCGACGTCGGCATGCGCCAGCTCGGCGGCGAGTCGATCATGCTGACCGGCGCCGAGATGCAGCTCGGCCGCGG
CGAGACCATCGCCGACACCGCGCGGGTGCTGTCGCGCTTCGTCGACATCATCATGATCCGCATCCTCAATCACGACTCGC
TGCTGGAGCTCGCCGCCAACGCTACCGTGCCGGTGATCAACGGGCTCACCCGCAAGTCGCATCCGTGCCAGGTGATGGCC
GACGTGATGACGTTCGAGGAGCATCGCGGCCCGATCAAGGGCGCGACCGTGGCCTGGACCGGCGACGACAACAACGTGCT
GGCGTCGTGGGCGCATGCCGCACAGCGGTTCGATTTCAGGCTCAACATCGCGACCCCGCCGCAGCTCGCGCCCAACAAGG
CGATGCGCGACTGGATCAAGACCAGTGGTGCTGCGATCACGCTCGGCAGCGACGCCGAGGCGGCGGTCCGCGGCGCCGAT
TGCGTCGTCACCGACACCTGGGTGTCGATGGGCGACAAGGACGGCGAGCACCGCCACAATCTGCTGAAGCCGTTTCAGGT
CAACGCCAAGCTGATGTCGCTCGCCCACAAGGACGCGATCTTCATGCACTGCCTGCCGGCGCATCGCGGCGAGGAGGTCA
CCGACGAGGTGATCGACGGCCCGCAATCGGTGGTGTTCGACGAAGCCGAAAACCGCCTGCACGCGCAGAAGGGCATCCTG
GCCTGGTGCCTCGGCGCGGTGGCGTAG

Upstream 100 bases:

>100_bases
CGCTGATCGTCGACGAGGTGGAGATCGACGAGGCGGTGACGCGGCTCGAGCGCGCCTGCGGCGTGTTGTCCGGCCAGGCC
GCGCAGCGGGAGGCGGTGTG

Downstream 100 bases:

>100_bases
CGGTCATCGCGACACATTCGGCGTCATCCTGAGGTGCCCGCGCGAAGCGCGGGCCTCGAAGGATGCTGAGACAGGGTGGT
GGCGACGCCGCCGCCCTTCG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSSATNRAVSSSKSTIESPAMTSAPRHFLDLTEVPTSELRNMLAAGVAMKAKRKANADADKPLAGKTLAMIFEKPSTRTR
VSFDVGMRQLGGESIMLTGAEMQLGRGETIADTARVLSRFVDIIMIRILNHDSLLELAANATVPVINGLTRKSHPCQVMA
DVMTFEEHRGPIKGATVAWTGDDNNVLASWAHAAQRFDFRLNIATPPQLAPNKAMRDWIKTSGAAITLGSDAEAAVRGAD
CVVTDTWVSMGDKDGEHRHNLLKPFQVNAKLMSLAHKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGIL
AWCLGAVA

Sequences:

>Translated_328_residues
MSSATNRAVSSSKSTIESPAMTSAPRHFLDLTEVPTSELRNMLAAGVAMKAKRKANADADKPLAGKTLAMIFEKPSTRTR
VSFDVGMRQLGGESIMLTGAEMQLGRGETIADTARVLSRFVDIIMIRILNHDSLLELAANATVPVINGLTRKSHPCQVMA
DVMTFEEHRGPIKGATVAWTGDDNNVLASWAHAAQRFDFRLNIATPPQLAPNKAMRDWIKTSGAAITLGSDAEAAVRGAD
CVVTDTWVSMGDKDGEHRHNLLKPFQVNAKLMSLAHKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGIL
AWCLGAVA
>Mature_327_residues
SSATNRAVSSSKSTIESPAMTSAPRHFLDLTEVPTSELRNMLAAGVAMKAKRKANADADKPLAGKTLAMIFEKPSTRTRV
SFDVGMRQLGGESIMLTGAEMQLGRGETIADTARVLSRFVDIIMIRILNHDSLLELAANATVPVINGLTRKSHPCQVMAD
VMTFEEHRGPIKGATVAWTGDDNNVLASWAHAAQRFDFRLNIATPPQLAPNKAMRDWIKTSGAAITLGSDAEAAVRGADC
VVTDTWVSMGDKDGEHRHNLLKPFQVNAKLMSLAHKDAIFMHCLPAHRGEEVTDEVIDGPQSVVFDEAENRLHAQKGILA
WCLGAVA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=292, Percent_Identity=39.3835616438356, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=24.9180327868852, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=38.4146341463415, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=37.9939209726444, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=29.6416938110749, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994908, Length=347, Percent_Identity=25.9365994236311, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=287, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322373, Length=312, Percent_Identity=35.2564102564103, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=26.4516129032258, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=23.9482200647249, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35489; Mature: 35358

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSATNRAVSSSKSTIESPAMTSAPRHFLDLTEVPTSELRNMLAAGVAMKAKRKANADAD
CCCCHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KPLAGKTLAMIFEKPSTRTRVSFDVGMRQLGGESIMLTGAEMQLGRGETIADTARVLSRF
CCCCCCEEEEEEECCCCCEEEEHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHHHH
VDIIMIRILNHDSLLELAANATVPVINGLTRKSHPCQVMADVMTFEEHRGPIKGATVAWT
HHHHHHHHHCCCHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
GDDNNVLASWAHAAQRFDFRLNIATPPQLAPNKAMRDWIKTSGAAITLGSDAEAAVRGAD
CCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHCCCC
CVVTDTWVSMGDKDGEHRHNLLKPFQVNAKLMSLAHKDAIFMHCLPAHRGEEVTDEVIDG
EEEEEHHHHCCCCCCHHHHHCCCCHHCCHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCC
PQSVVFDEAENRLHAQKGILAWCLGAVA
CHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSATNRAVSSSKSTIESPAMTSAPRHFLDLTEVPTSELRNMLAAGVAMKAKRKANADAD
CCCHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KPLAGKTLAMIFEKPSTRTRVSFDVGMRQLGGESIMLTGAEMQLGRGETIADTARVLSRF
CCCCCCEEEEEEECCCCCEEEEHHHHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHHHH
VDIIMIRILNHDSLLELAANATVPVINGLTRKSHPCQVMADVMTFEEHRGPIKGATVAWT
HHHHHHHHHCCCHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
GDDNNVLASWAHAAQRFDFRLNIATPPQLAPNKAMRDWIKTSGAAITLGSDAEAAVRGAD
CCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHCCCC
CVVTDTWVSMGDKDGEHRHNLLKPFQVNAKLMSLAHKDAIFMHCLPAHRGEEVTDEVIDG
EEEEEHHHHCCCCCCHHHHHCCCCHHCCHHHHHHHHHHHHEEEECCCCCCCHHHHHHHCC
PQSVVFDEAENRLHAQKGILAWCLGAVA
CHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA