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The map label for this gene is rutF [H]

Identifier: 86747823

GI number: 86747823

Start: 784293

End: 784817

Strand: Direct

Name: rutF [H]

Synonym: RPB_0697

Alternate gene names: 86747823

Gene position: 784293-784817 (Clockwise)

Preceding gene: 86747822

Following gene: 86747825

Centisome position: 14.71

GC content: 63.05

Gene sequence:

>525_bases
ATGGCGACGACTGAAGTGGCAACGGACGGAGAGATGCCGATCTCGTCGGCTGTCTTCAAGCAGAGCATGCGCCTTCTGGC
CGGCGGCGTCTGCATTGTCGCGAGTGCCAAGGATGGCGAGCGGCTAGGACTCACCATGACGGCGGTCTGTTCGTTGACGC
TCGACCCGCCTACGCTGATAGCTTGCGTCAACCGCGACGCAGGCGCCCACGCCTTGATGCGTTCGACCAGACGCATCAGC
GTGAATCTGCTAGGCGCGGACCACGCCGAACTCGCCGAACGGTTCGCGTCGTCGAGCATCAAGGGGGCCGCGCGGTTCGA
CCTCGCGACATGGGTCGACATGGAAAGCGGTGTCCCGGCATTGGCGGACGCGTTGGCCGTGCTGGACTGCGAGATTTTCG
AAGAAACCTCGGTCGGCCAGCATTCGGTGTTCTTCTGCGAGGTGAAAGCCGCCCGTGTTCAGCCGGCGAAGGAACCGCTG
GTGCATTTCAATCGCGAGTTCCGCGGATTGATACCGGTCGGCTGA

Upstream 100 bases:

>100_bases
TGGCACGACGGCGGGGAAACGCGGCCCCGCGCGGACTTGCTCTATCCGGCCTCTCGCAGATCGGATCCCAGAACATCAAT
CATCTTCGAGGCAGTTCGAC

Downstream 100 bases:

>100_bases
CCTCACCCCGCCTTGCGGGCCTTGCCGCCGGCGGTGCTGCGTGCGGGCTTCTTTGCCTTGCCGGCCTCCTCTTTCGAGCC
GGCGGTCTTGGCGCCGCTGA

Product: flavin reductase-like, FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MATTEVATDGEMPISSAVFKQSMRLLAGGVCIVASAKDGERLGLTMTAVCSLTLDPPTLIACVNRDAGAHALMRSTRRIS
VNLLGADHAELAERFASSSIKGAARFDLATWVDMESGVPALADALAVLDCEIFEETSVGQHSVFFCEVKAARVQPAKEPL
VHFNREFRGLIPVG

Sequences:

>Translated_174_residues
MATTEVATDGEMPISSAVFKQSMRLLAGGVCIVASAKDGERLGLTMTAVCSLTLDPPTLIACVNRDAGAHALMRSTRRIS
VNLLGADHAELAERFASSSIKGAARFDLATWVDMESGVPALADALAVLDCEIFEETSVGQHSVFFCEVKAARVQPAKEPL
VHFNREFRGLIPVG
>Mature_173_residues
ATTEVATDGEMPISSAVFKQSMRLLAGGVCIVASAKDGERLGLTMTAVCSLTLDPPTLIACVNRDAGAHALMRSTRRISV
NLLGADHAELAERFASSSIKGAARFDLATWVDMESGVPALADALAVLDCEIFEETSVGQHSVFFCEVKAARVQPAKEPLV
HFNREFRGLIPVG

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=32.2784810126582, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18490; Mature: 18359

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTEVATDGEMPISSAVFKQSMRLLAGGVCIVASAKDGERLGLTMTAVCSLTLDPPTLI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEHEEEEECCCCEEE
ACVNRDAGAHALMRSTRRISVNLLGADHAELAERFASSSIKGAARFDLATWVDMESGVPA
EEECCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHEEEECCCCCHH
LADALAVLDCEIFEETSVGQHSVFFCEVKAARVQPAKEPLVHFNREFRGLIPVG
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCCHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
ATTEVATDGEMPISSAVFKQSMRLLAGGVCIVASAKDGERLGLTMTAVCSLTLDPPTLI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEHEEEEECCCCEEE
ACVNRDAGAHALMRSTRRISVNLLGADHAELAERFASSSIKGAARFDLATWVDMESGVPA
EEECCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHEEEECCCCCHH
LADALAVLDCEIFEETSVGQHSVFFCEVKAARVQPAKEPLVHFNREFRGLIPVG
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHCCCCHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA