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The map label for this gene is hisA [H]

Identifier: 86747534

GI number: 86747534

Start: 452026

End: 452763

Strand: Direct

Name: hisA [H]

Synonym: RPB_0408

Alternate gene names: 86747534

Gene position: 452026-452763 (Clockwise)

Preceding gene: 86747533

Following gene: 86747535

Centisome position: 8.48

GC content: 66.8

Gene sequence:

>738_bases
ATGATTCTCTTCCCCGCGATCGATCTCAAGAACGGCCAGTGCGTGCGGCTCGAACAGGGCGACATGGCGCGCGCCACCGT
GTTCAACCTCGATCCGACCGCGCAGGCGAAGAGCTTTGCGGCGCAGGGTTTTCAGTATCTCCATGTGGTCGATCTCGACG
GCGCCTTCGCCGGCAAGCCGATGAACGCACAGGCCGTGGAATCGATGCTGAAAGTCGTGTCGATGCCGGTCCAGCTCGGC
GGCGGCATCCGCGACCTCGCGACAGTCGAGGCCTGGCTGTCCAAAGGCATCGCCCGCGTCATCATCGGCACCGCGGCGGT
GCGGGATCCGGCGCTGGTGAAGCAAGCTGCGAAGTCGTTCCCCGGCCGCGTCGCGGTCGGCCTAGATGCCCGCGACGGCA
AGGTTGCGGTCGAAGGCTGGGCGGAGAGCTCGCAGGTCACCGCGCTGGAAATTGCGCAACGCTTCGAAGACGCCGGCGTC
GCCGCGATCATCTTCACCGACATTGCCCGCGACGGCCTGCTCAAGGGCATCAACTGGGATGCGACGATCGCGCTCGCCGA
GGCCATCAGTATTCCGGTAATCGCCTCCGGGGGGCTCGCCTCGATCGAGGATGTGAAGGCGATGCTGAGCCCGCGCGCTC
ACAAACTGGAAGGCGCGATCGCCGGCCGTGCGCTGTATGACGGCCGGCTCGACCCGGCGGAAGCGCTGGCGCTGATCGGC
GCCGCCAGAGCGGCTTGA

Upstream 100 bases:

>100_bases
CGGCCTCGAAGGGTGGCCGCGCACACCGGATCAGCATCCTTCGAGGCGCACCGCTTGCGCGGCGCGCACCTCAGGATAAC
GCTGGAACAGGTTGCCATTC

Downstream 100 bases:

>100_bases
GGACGAGCGATGTTCAAGGTCCGCGTCATTCCCTGCCTCGACGTCAAGGACGGCCGCGTCGTCAAGGGCGTCAACTTCGT
CGATCTGCGCGACGCCGGTG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MILFPAIDLKNGQCVRLEQGDMARATVFNLDPTAQAKSFAAQGFQYLHVVDLDGAFAGKPMNAQAVESMLKVVSMPVQLG
GGIRDLATVEAWLSKGIARVIIGTAAVRDPALVKQAAKSFPGRVAVGLDARDGKVAVEGWAESSQVTALEIAQRFEDAGV
AAIIFTDIARDGLLKGINWDATIALAEAISIPVIASGGLASIEDVKAMLSPRAHKLEGAIAGRALYDGRLDPAEALALIG
AARAA

Sequences:

>Translated_245_residues
MILFPAIDLKNGQCVRLEQGDMARATVFNLDPTAQAKSFAAQGFQYLHVVDLDGAFAGKPMNAQAVESMLKVVSMPVQLG
GGIRDLATVEAWLSKGIARVIIGTAAVRDPALVKQAAKSFPGRVAVGLDARDGKVAVEGWAESSQVTALEIAQRFEDAGV
AAIIFTDIARDGLLKGINWDATIALAEAISIPVIASGGLASIEDVKAMLSPRAHKLEGAIAGRALYDGRLDPAEALALIG
AARAA
>Mature_245_residues
MILFPAIDLKNGQCVRLEQGDMARATVFNLDPTAQAKSFAAQGFQYLHVVDLDGAFAGKPMNAQAVESMLKVVSMPVQLG
GGIRDLATVEAWLSKGIARVIIGTAAVRDPALVKQAAKSFPGRVAVGLDARDGKVAVEGWAESSQVTALEIAQRFEDAGV
AAIIFTDIARDGLLKGINWDATIALAEAISIPVIASGGLASIEDVKAMLSPRAHKLEGAIAGRALYDGRLDPAEALALIG
AARAA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=247, Percent_Identity=35.6275303643725, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1788336, Length=248, Percent_Identity=25.4032258064516, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: =5.3.1.16 [H]

Molecular weight: Translated: 25500; Mature: 25500

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFPAIDLKNGQCVRLEQGDMARATVFNLDPTAQAKSFAAQGFQYLHVVDLDGAFAGKP
CEEECEEECCCCCEEEECCCCCCEEEEEECCCCHHHHHHHHCCEEEEEEEECCCCCCCCC
MNAQAVESMLKVVSMPVQLGGGIRDLATVEAWLSKGIARVIIGTAAVRDPALVKQAAKSF
CCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCC
PGRVAVGLDARDGKVAVEGWAESSQVTALEIAQRFEDAGVAAIIFTDIARDGLLKGINWD
CCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCC
ATIALAEAISIPVIASGGLASIEDVKAMLSPRAHKLEGAIAGRALYDGRLDPAEALALIG
HHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCHHHHHHHHH
AARAA
HHCCC
>Mature Secondary Structure
MILFPAIDLKNGQCVRLEQGDMARATVFNLDPTAQAKSFAAQGFQYLHVVDLDGAFAGKP
CEEECEEECCCCCEEEECCCCCCEEEEEECCCCHHHHHHHHCCEEEEEEEECCCCCCCCC
MNAQAVESMLKVVSMPVQLGGGIRDLATVEAWLSKGIARVIIGTAAVRDPALVKQAAKSF
CCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCC
PGRVAVGLDARDGKVAVEGWAESSQVTALEIAQRFEDAGVAAIIFTDIARDGLLKGINWD
CCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCC
ATIALAEAISIPVIASGGLASIEDVKAMLSPRAHKLEGAIAGRALYDGRLDPAEALALIG
HHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCHHHHHHHHH
AARAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA