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The map label for this gene is hisH [H]

Identifier: 86747533

GI number: 86747533

Start: 451218

End: 451868

Strand: Direct

Name: hisH [H]

Synonym: RPB_0407

Alternate gene names: 86747533

Gene position: 451218-451868 (Clockwise)

Preceding gene: 86747532

Following gene: 86747534

Centisome position: 8.46

GC content: 65.9

Gene sequence:

>651_bases
ATGAGCGTGGCCATCGTCGATTACGGCTCGGGCAATCTGCACTCGGCCGCGAAGGCGTTCGAGCGTGCGGCGCGGAGCAT
GGAAGTCCCCGAAAAGATCATCGTCACCCGCGATCCGGAGCAGGTGTTTCGCTCCGATCGCGTGGTGCTGCCGGGCGTCG
GCGCTTTCGCCGATTGCCGCAAGGGGCTCGACGCGATCGACGGCATGGTCGAGGCGTTGAACGAGACGGTGCGGGTCAAG
GCGCGGCCGTTCTTCGGCATCTGCGTCGGCATGCAGCTGATGGCGACCCGCGGCAAGGAGCACGTCACCACCGACGGGCT
CGGCTGGATTCCCGGCGACGTCGTCAGGATCGCGCCGAACCAGGAGGATCTGAAGATCCCGCATATGGGCTGGAACACGC
TCGACGTGTTGCGCGAGCACCCGGTGCTGGAGCGGCTGCCGCTCGGGCCGAAGGGCTTGCATGCGTACTTCGTGCATTCG
TTTCATCTGGCCGCTACCAGCGAAGCCGACGTGCTGGCCCGCGCCGACTACGGCGGCCCGGTCACCGCGGTGGTGGGCCG
CGACACCATGCTGGGAACCCAGTTTCACCCCGAGAAAAGCCAGCGTTTCGGGCTGGCGCTGATCTCGAATTTTCTGAAGT
GGAAGCCGTGA

Upstream 100 bases:

>100_bases
TCGACCGCGGCGCGCCGCCGCCGACCCGTAGCGTCCCGATGCCGTCGTCGTCGATCTATGGCGACATCGTCGGCTCGTTC
CCGCGCCCTGGATCGTCGCG

Downstream 100 bases:

>100_bases
TGGGCTTTCGCTTTCATCCGGACACAGCCGTCACCCTGAGGTGCCGTCGCGAAGCGACGGCCTCGAAGGGTGGCCGCGCA
CACCGGATCAGCATCCTTCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSVAIVDYGSGNLHSAAKAFERAARSMEVPEKIIVTRDPEQVFRSDRVVLPGVGAFADCRKGLDAIDGMVEALNETVRVK
ARPFFGICVGMQLMATRGKEHVTTDGLGWIPGDVVRIAPNQEDLKIPHMGWNTLDVLREHPVLERLPLGPKGLHAYFVHS
FHLAATSEADVLARADYGGPVTAVVGRDTMLGTQFHPEKSQRFGLALISNFLKWKP

Sequences:

>Translated_216_residues
MSVAIVDYGSGNLHSAAKAFERAARSMEVPEKIIVTRDPEQVFRSDRVVLPGVGAFADCRKGLDAIDGMVEALNETVRVK
ARPFFGICVGMQLMATRGKEHVTTDGLGWIPGDVVRIAPNQEDLKIPHMGWNTLDVLREHPVLERLPLGPKGLHAYFVHS
FHLAATSEADVLARADYGGPVTAVVGRDTMLGTQFHPEKSQRFGLALISNFLKWKP
>Mature_215_residues
SVAIVDYGSGNLHSAAKAFERAARSMEVPEKIIVTRDPEQVFRSDRVVLPGVGAFADCRKGLDAIDGMVEALNETVRVKA
RPFFGICVGMQLMATRGKEHVTTDGLGWIPGDVVRIAPNQEDLKIPHMGWNTLDVLREHPVLERLPLGPKGLHAYFVHSF
HLAATSEADVLARADYGGPVTAVVGRDTMLGTQFHPEKSQRFGLALISNFLKWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=218, Percent_Identity=37.1559633027523, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=224, Percent_Identity=29.4642857142857, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23682; Mature: 23550

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIVDYGSGNLHSAAKAFERAARSMEVPEKIIVTRDPEQVFRSDRVVLPGVGAFADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCCCHHHHHH
KGLDAIDGMVEALNETVRVKARPFFGICVGMQLMATRGKEHVTTDGLGWIPGDVVRIAPN
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
QEDLKIPHMGWNTLDVLREHPVLERLPLGPKGLHAYFVHSFHLAATSEADVLARADYGGP
CCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCCHHEEEHEEEEEEECCCCCEEEECCCCCC
VTAVVGRDTMLGTQFHPEKSQRFGLALISNFLKWKP
EEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVAIVDYGSGNLHSAAKAFERAARSMEVPEKIIVTRDPEQVFRSDRVVLPGVGAFADCR
EEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCCCHHHHHH
KGLDAIDGMVEALNETVRVKARPFFGICVGMQLMATRGKEHVTTDGLGWIPGDVVRIAPN
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
QEDLKIPHMGWNTLDVLREHPVLERLPLGPKGLHAYFVHSFHLAATSEADVLARADYGGP
CCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCCHHEEEHEEEEEEECCCCCEEEECCCCCC
VTAVVGRDTMLGTQFHPEKSQRFGLALISNFLKWKP
EEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA