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The map label for this gene is trmE

Identifier: 86747518

GI number: 86747518

Start: 436875

End: 438257

Strand: Reverse

Name: trmE

Synonym: RPB_0392

Alternate gene names: 86747518

Gene position: 438257-436875 (Counterclockwise)

Preceding gene: 86747519

Following gene: 86747517

Centisome position: 8.22

GC content: 68.11

Gene sequence:

>1383_bases
ATGCATCCGAACGACCAGACCATCTTCGCCCTCGCGTCCGGCCGGCTGCCGTCGGCGCTGGCGATCGTGCGGGTGTCGGG
CGCGCGCGCCAGGGACGTGCTGGCCGCGCTGACGGGGGCATTGCCGCCGCCGCGGACTGTGCGGCGGGTTCGGATTCGCG
ATGTAAATCATGAGCTTATCGACGACGCCGTGGTGCTGTGGTTCGCCGCGCCGGCGAGCGCGACCGGCGAGGATGTGGCC
GAGTTTCACATCCATGGCGGCCGCGCGGTGCTGGCCGCGTTGGTCAAGGCTTTGGCTTCCTTCGACGATGTGCGGCCGGC
GGAGCCGGGCGAGTTCACGCGGCGCGCATTCGAGAACGGCAAGCTCGATCTCACCGAAGCCGAAGGGCTCGACGATCTGA
TTCACGCCGACACCGAGGCGCAGCGACGGCTCGCGGTGCGGCAGCTCGGCGGTCTGCTCGGCGACAGGGCGCGGCGATGG
CGGGCGCAGATCATCGAGGCGCTGGCACTGATCGAGGCCGGGATCGATTTCGCCGACGAAGGCGACGTGCAGGGCGAGCT
GATGGCGCCGGCGCTGCGGACGATCGCGACGCTGCACGGCGAGATTGCAGAAGTGCTTGCGGCACAGGGCAAAAGCGAAC
GGCTGCGGGACGGGCTGGTGGTCGCGATCGCGGGGCCGCCGAATGTCGGCAAATCGACGCTGATCAATCGTCTGGCGCGC
CGCGAGGTCGCGATCGTGTCGCCGCATGCCGGCACGACGCGTGATGTGATCGAGGTGCAGCTCGATCTCGGCGGTTATCC
GGTGACGTTGATCGACACTGCGGGGATTCGTGAGAGCGAGGATTCGGTCGAGCAGGAGGGCGTGCGCCGGGCGAAGGCGC
GAGCCGCGGAGGCGGACCTGGTGTTGTGGCTCGGCGCGGATGAGGCGATCGACGTAGCGGTCGAGGGCTCGGCGCCAGTC
TGGCGGGTGCGGAACAAGATCGATTTGGTGCCGCAGGCCGATACCGTTGGAGCCGATTCTGGCGGCGCGGGTCTTGCGGC
AAACCTGTGGCAAACCGAAGGCAACGTTGCCGCAAGGGAAGCGTTTGGGATTTCGGCGAAGCGGGGCGATGGCGTCGGCG
ATATGGTCGAGGCGCTGGCGGGATTCGCTGCGCAGTATTTTGCGGCGAGCGGGGAGGCCGCGGTGATCAGCCGGGAGCGG
CATCGGCTTTTGCTGCAACAGGCCGAGGTCATGCTGCGGTGCAGCATGACTGTGGGCTTGGCACCGGAGTTGGTTGCGGA
GGAACTCAGGCTGGCTGCGGGAGCGCTGGGGCGGCTGCTCGGCCGGGTCGATGTCGAAGACGTGCTCGGCGAGATCTTTG
GCCGGTTCTGCATCGGCAAGTGA

Upstream 100 bases:

>100_bases
GGTTGGAAGCAAGTCTGCGAGGGCGCCCCGAAACGGGCGCCCTTTTTGCTGCGTGCGATTGAGCGTTGCGTCGGACGGTG
GCACTCGGCAAGACAGACCG

Downstream 100 bases:

>100_bases
GATTGTCGAGAATATATAAATATAAATATATTCATTTCTTGATAGGTTCCGATCGAAGCCCGACCGGCAGGCCGAAACCG
GATTTCGACTGCGATCTGGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MHPNDQTIFALASGRLPSALAIVRVSGARARDVLAALTGALPPPRTVRRVRIRDVNHELIDDAVVLWFAAPASATGEDVA
EFHIHGGRAVLAALVKALASFDDVRPAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTEAQRRLAVRQLGGLLGDRARRW
RAQIIEALALIEAGIDFADEGDVQGELMAPALRTIATLHGEIAEVLAAQGKSERLRDGLVVAIAGPPNVGKSTLINRLAR
REVAIVSPHAGTTRDVIEVQLDLGGYPVTLIDTAGIRESEDSVEQEGVRRAKARAAEADLVLWLGADEAIDVAVEGSAPV
WRVRNKIDLVPQADTVGADSGGAGLAANLWQTEGNVAAREAFGISAKRGDGVGDMVEALAGFAAQYFAASGEAAVISRER
HRLLLQQAEVMLRCSMTVGLAPELVAEELRLAAGALGRLLGRVDVEDVLGEIFGRFCIGK

Sequences:

>Translated_460_residues
MHPNDQTIFALASGRLPSALAIVRVSGARARDVLAALTGALPPPRTVRRVRIRDVNHELIDDAVVLWFAAPASATGEDVA
EFHIHGGRAVLAALVKALASFDDVRPAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTEAQRRLAVRQLGGLLGDRARRW
RAQIIEALALIEAGIDFADEGDVQGELMAPALRTIATLHGEIAEVLAAQGKSERLRDGLVVAIAGPPNVGKSTLINRLAR
REVAIVSPHAGTTRDVIEVQLDLGGYPVTLIDTAGIRESEDSVEQEGVRRAKARAAEADLVLWLGADEAIDVAVEGSAPV
WRVRNKIDLVPQADTVGADSGGAGLAANLWQTEGNVAAREAFGISAKRGDGVGDMVEALAGFAAQYFAASGEAAVISRER
HRLLLQQAEVMLRCSMTVGLAPELVAEELRLAAGALGRLLGRVDVEDVLGEIFGRFCIGK
>Mature_460_residues
MHPNDQTIFALASGRLPSALAIVRVSGARARDVLAALTGALPPPRTVRRVRIRDVNHELIDDAVVLWFAAPASATGEDVA
EFHIHGGRAVLAALVKALASFDDVRPAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTEAQRRLAVRQLGGLLGDRARRW
RAQIIEALALIEAGIDFADEGDVQGELMAPALRTIATLHGEIAEVLAAQGKSERLRDGLVVAIAGPPNVGKSTLINRLAR
REVAIVSPHAGTTRDVIEVQLDLGGYPVTLIDTAGIRESEDSVEQEGVRRAKARAAEADLVLWLGADEAIDVAVEGSAPV
WRVRNKIDLVPQADTVGADSGGAGLAANLWQTEGNVAAREAFGISAKRGDGVGDMVEALAGFAAQYFAASGEAAVISRER
HRLLLQQAEVMLRCSMTVGLAPELVAEELRLAAGALGRLLGRVDVEDVLGEIFGRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=41.4893617021277, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=41.5217391304348, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=39.2430278884462, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI2367268, Length=478, Percent_Identity=36.1924686192469, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI87082120, Length=168, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=468, Percent_Identity=32.9059829059829, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6323665, Length=496, Percent_Identity=33.0645161290323, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=38.125, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_RHOP2 (Q2J357)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484014.1
- ProteinModelPortal:   Q2J357
- STRING:   Q2J357
- GeneID:   3908830
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0392
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   REIAITS
- ProtClustDB:   PRK05291
- BioCyc:   RPAL316058:RPB_0392-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR003593
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- SMART:   SM00382
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48823; Mature: 48823

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 81-81 BINDING 121-121 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPNDQTIFALASGRLPSALAIVRVSGARARDVLAALTGALPPPRTVRRVRIRDVNHELI
CCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHH
DDAVVLWFAAPASATGEDVAEFHIHGGRAVLAALVKALASFDDVRPAEPGEFTRRAFENG
HHHEEEEEECCCCCCCCHHHHHEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCC
KLDLTEAEGLDDLIHADTEAQRRLAVRQLGGLLGDRARRWRAQIIEALALIEAGIDFADE
CEEEEHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDVQGELMAPALRTIATLHGEIAEVLAAQGKSERLRDGLVVAIAGPPNVGKSTLINRLAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHH
REVAIVSPHAGTTRDVIEVQLDLGGYPVTLIDTAGIRESEDSVEQEGVRRAKARAAEADL
CCEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCE
VLWLGADEAIDVAVEGSAPVWRVRNKIDLVPQADTVGADSGGAGLAANLWQTEGNVAARE
EEEECCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEHHEECCCCEEHHH
AFGISAKRGDGVGDMVEALAGFAAQYFAASGEAAVISRERHRLLLQQAEVMLRCSMTVGL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
APELVAEELRLAAGALGRLLGRVDVEDVLGEIFGRFCIGK
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHPNDQTIFALASGRLPSALAIVRVSGARARDVLAALTGALPPPRTVRRVRIRDVNHELI
CCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHH
DDAVVLWFAAPASATGEDVAEFHIHGGRAVLAALVKALASFDDVRPAEPGEFTRRAFENG
HHHEEEEEECCCCCCCCHHHHHEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCC
KLDLTEAEGLDDLIHADTEAQRRLAVRQLGGLLGDRARRWRAQIIEALALIEAGIDFADE
CEEEEHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GDVQGELMAPALRTIATLHGEIAEVLAAQGKSERLRDGLVVAIAGPPNVGKSTLINRLAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHH
REVAIVSPHAGTTRDVIEVQLDLGGYPVTLIDTAGIRESEDSVEQEGVRRAKARAAEADL
CCEEEECCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCE
VLWLGADEAIDVAVEGSAPVWRVRNKIDLVPQADTVGADSGGAGLAANLWQTEGNVAARE
EEEECCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEHHEECCCCEEHHH
AFGISAKRGDGVGDMVEALAGFAAQYFAASGEAAVISRERHRLLLQQAEVMLRCSMTVGL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
APELVAEELRLAAGALGRLLGRVDVEDVLGEIFGRFCIGK
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA