Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 86747519

GI number: 86747519

Start: 438366

End: 439631

Strand: Reverse

Name: rho [H]

Synonym: RPB_0393

Alternate gene names: 86747519

Gene position: 439631-438366 (Counterclockwise)

Preceding gene: 86747520

Following gene: 86747518

Centisome position: 8.25

GC content: 62.01

Gene sequence:

>1266_bases
ATGCGGGAAATGAAACTTTCAGACCTCAAGGCCAAGACGCCGGCCGAGCTCGTCTCGTTCGCCGAAGAGCTGGGGGTCGA
GAATGCCAGCACGATGCGCAAGCAGGAGCTGATGTTCGCCTGCCTGAAGCAACTGTCGGCCAAGGAAACCGATATCATCG
GTGAAGGCGTCGTCGAGGTTCTGTCCGACGGCTTCGGCTTCCTGCGTTCGCCCGATGCCAACTATTTGCCGGGTCCGGAC
GACATCTACGTTTCGCCGTCCCAGATCCGCCGTTTTGGCCTGCGCACCGGCGACACCATCGAGGGCCACATCCGCAGTCC
GAAGGAAGGCGAACGCTACTTCGCGCTGCTCAAGGTCAATACGCTGAACTTCGAGGACCCCGAGAAGTCCAAGCACAAGG
TCAATTTCGACAATCTGACGCCGCTGTTTCCGGACGAGCGCTTCCGGCTCGAGATCGACGATCCGACCCGCAAGGATCTG
TCGGCGCGGGTGATCGACATCGTGGCGCCGATCGGCAAGGGTCAGCGCGCGCTGATCGTGGCGCCGCCGCGCACAGGCAA
GACCGTGCTGATGCAGAACATCGCGCATTCGATCACCGCCAATCATCCGGAATGCTATCTGATCGTGCTGCTGATCGACG
AGCGGCCGGAAGAAGTCACCGACATGCAGCGCTCGGTGAAGGGCGAGGTGGTGTCCTCGACCTTCGACGAACCCGCGGTG
CGCCACGTCCAGGTCGCCGAGATGGTGATCGAGAAGGCCAAGCGGCTGGTCGAACACGGCCGCGACGTCGTCATCCTGCT
CGACTCGATCACGCGTCTGGGCCGCGCCTACAACACGGTGGTGCCGTCCTCCGGCAAGGTGCTGACCGGCGGCGTCGACG
CCAATGCGCTGCAGCGGCCGAAGCGCTTTTTCGGCGCCGCGCGCAACATCGAAGAGGGCGGCTCGCTCACCATCATCGCC
ACCGCGCTGGTCGATACCGGCTCGCGCATGGACGAAGTGATCTTCGAAGAGTTCAAGGGCACGGGTAACAGCGAACTGAT
CCTCGACCGCAAGGTCTCGGACAAGCGCACCTTCCCGGCGATAGACATCTCGCGCTCCGGCACCCGCAAGGAAGAGCTGA
TCACCGATCCGCAACTCTTGAAGAAGATGTACGTGCTGCGCCGCATCCTCAATCCGATGGGCACCATGGACGCGATCGAC
TTCCTGCTCGACAAGCTGCGCAACACCAAGAACAACGCCGAATTCTTCGAGTCGATGAACACCTGA

Upstream 100 bases:

>100_bases
CATCGACCCGAAGACCTGCGGCCTCTCTGTATTTTCCAGTCCCCCGCTCTCTTCCGCTGACGAATCCCCTCTCTCGGTTT
TATTCCACAGGACCATCCCA

Downstream 100 bases:

>100_bases
TCGACCGCGGTTGGAAGCAAGTCTGCGAGGGCGCCCCGAAACGGGCGCCCTTTTTGCTGCGTGCGATTGAGCGTTGCGTC
GGACGGTGGCACTCGGCAAG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MREMKLSDLKAKTPAELVSFAEELGVENASTMRKQELMFACLKQLSAKETDIIGEGVVEVLSDGFGFLRSPDANYLPGPD
DIYVSPSQIRRFGLRTGDTIEGHIRSPKEGERYFALLKVNTLNFEDPEKSKHKVNFDNLTPLFPDERFRLEIDDPTRKDL
SARVIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAV
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALVDTGSRMDEVIFEEFKGTGNSELILDRKVSDKRTFPAIDISRSGTRKEELITDPQLLKKMYVLRRILNPMGTMDAID
FLLDKLRNTKNNAEFFESMNT

Sequences:

>Translated_421_residues
MREMKLSDLKAKTPAELVSFAEELGVENASTMRKQELMFACLKQLSAKETDIIGEGVVEVLSDGFGFLRSPDANYLPGPD
DIYVSPSQIRRFGLRTGDTIEGHIRSPKEGERYFALLKVNTLNFEDPEKSKHKVNFDNLTPLFPDERFRLEIDDPTRKDL
SARVIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAV
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALVDTGSRMDEVIFEEFKGTGNSELILDRKVSDKRTFPAIDISRSGTRKEELITDPQLLKKMYVLRRILNPMGTMDAID
FLLDKLRNTKNNAEFFESMNT
>Mature_421_residues
MREMKLSDLKAKTPAELVSFAEELGVENASTMRKQELMFACLKQLSAKETDIIGEGVVEVLSDGFGFLRSPDANYLPGPD
DIYVSPSQIRRFGLRTGDTIEGHIRSPKEGERYFALLKVNTLNFEDPEKSKHKVNFDNLTPLFPDERFRLEIDDPTRKDL
SARVIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAV
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALVDTGSRMDEVIFEEFKGTGNSELILDRKVSDKRTFPAIDISRSGTRKEELITDPQLLKKMYVLRRILNPMGTMDAID
FLLDKLRNTKNNAEFFESMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=69.2307692307692, Blast_Score=609, Evalue=1e-175,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47091; Mature: 47091

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREMKLSDLKAKTPAELVSFAEELGVENASTMRKQELMFACLKQLSAKETDIIGEGVVEV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LSDGFGFLRSPDANYLPGPDDIYVSPSQIRRFGLRTGDTIEGHIRSPKEGERYFALLKVN
HHCCCCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
TLNFEDPEKSKHKVNFDNLTPLFPDERFRLEIDDPTRKDLSARVIDIVAPIGKGQRALIV
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEE
APPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAV
ECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCHHH
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNSELILDRKVSDKRTFPA
HHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
IDISRSGTRKEELITDPQLLKKMYVLRRILNPMGTMDAIDFLLDKLRNTKNNAEFFESMN
EECCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCC
T
C
>Mature Secondary Structure
MREMKLSDLKAKTPAELVSFAEELGVENASTMRKQELMFACLKQLSAKETDIIGEGVVEV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LSDGFGFLRSPDANYLPGPDDIYVSPSQIRRFGLRTGDTIEGHIRSPKEGERYFALLKVN
HHCCCCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
TLNFEDPEKSKHKVNFDNLTPLFPDERFRLEIDDPTRKDLSARVIDIVAPIGKGQRALIV
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEE
APPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPAV
ECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCHHH
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNSELILDRKVSDKRTFPA
HHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCE
IDISRSGTRKEELITDPQLLKKMYVLRRILNPMGTMDAIDFLLDKLRNTKNNAEFFESMN
EECCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]