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The map label for this gene is dapF

Identifier: 86747485

GI number: 86747485

Start: 401137

End: 402018

Strand: Direct

Name: dapF

Synonym: RPB_0359

Alternate gene names: 86747485

Gene position: 401137-402018 (Clockwise)

Preceding gene: 86747482

Following gene: 86747486

Centisome position: 7.52

GC content: 68.14

Gene sequence:

>882_bases
ATGAGCGCGCTGGACAATCGCCTTTTCGCCAAGATGAACGGCATCGGCAACGAGATCGTCGTGGTCGATCTGCGCGACCA
GCCCGCGCCGGTGACGCCGGACGATGCCCGCGCGGTGGCGGCGCATGTGCCTTATGATCAACTGATGTTGCTGCAGCCGG
CGCGGCTGCCCGGCACCGAAGCCTTCGTCCGGATCTACAACAATGACGGCTCCGAATCCGGCGCTTGTGGCAACGGCATG
CGCTGCGTCGCGCGGCAGCTGTTCGCCGGCTCCGACCAGCAGGGCCTGACCTTCGAGACCCGCGCCGGCCTGCTCAATTG
CTGGCGCGGCCCCGCCGAGGGCCTGTTCACCGTCGACATGGGCGCGCCGAAATTCGGCTGGCAGGACATTCCGCTCGCCG
AGGAATTCCGCGACACCCGCGGCATCGAACTGCAGATCGGCCCGATCGACGCGCCGATCCTGCACACGCCCTCGGTGGTC
AGCATGGGCAATCCGCATGCGATCTTCTGGGTCGACGACATCCACGCCTACGATCTCGGCCAATTCGGACCGCTGCTGGA
GAATCACCCGATCTTTCCGGAGCGCGCCAATATCACGCTCGCCCATATCGTCGATCGCCAGCACATCACGATGCGGACCT
GGGAGCGCGGCGCCGGGCTGACCCGCGCCTGCGGCTCGGCCGCCTGCGCCACCGCGGTCGCCGCCGCGCGGCTGCGGCGG
ACCGACCGAATCGTCGAGATGACGCTGCCGGGCGGCAAGCTCACGATCGAATGGCGCGAGGGCGACGATCACGTGCTGAT
GACCGGCGGCGCCGAGCTCGAATTCGAGGGCCGGTTCGATCCGGCGCTGTTCGTCGGCGCGTGTGACACGACCGCCGCCT
GA

Upstream 100 bases:

>100_bases
GATCGGCGTGTGGCCTGACTGTGGCGGTCTGCCGACGCGCGCCGCCGGACTGGTCAAAACCGCCGTGCTTCGCCATATAG
GCCCCGACAAGGACCGGATC

Downstream 100 bases:

>100_bases
TGCCGGTCGAGATCGTCACCTTCGGCTGCCGCCTCAACGCCTTCGAATCCGAAGTGATCCGCCGCGAGGCGGAGGGCGCC
GGCCTTACCGAGACGATCGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSALDNRLFAKMNGIGNEIVVVDLRDQPAPVTPDDARAVAAHVPYDQLMLLQPARLPGTEAFVRIYNNDGSESGACGNGM
RCVARQLFAGSDQQGLTFETRAGLLNCWRGPAEGLFTVDMGAPKFGWQDIPLAEEFRDTRGIELQIGPIDAPILHTPSVV
SMGNPHAIFWVDDIHAYDLGQFGPLLENHPIFPERANITLAHIVDRQHITMRTWERGAGLTRACGSAACATAVAAARLRR
TDRIVEMTLPGGKLTIEWREGDDHVLMTGGAELEFEGRFDPALFVGACDTTAA

Sequences:

>Translated_293_residues
MSALDNRLFAKMNGIGNEIVVVDLRDQPAPVTPDDARAVAAHVPYDQLMLLQPARLPGTEAFVRIYNNDGSESGACGNGM
RCVARQLFAGSDQQGLTFETRAGLLNCWRGPAEGLFTVDMGAPKFGWQDIPLAEEFRDTRGIELQIGPIDAPILHTPSVV
SMGNPHAIFWVDDIHAYDLGQFGPLLENHPIFPERANITLAHIVDRQHITMRTWERGAGLTRACGSAACATAVAAARLRR
TDRIVEMTLPGGKLTIEWREGDDHVLMTGGAELEFEGRFDPALFVGACDTTAA
>Mature_292_residues
SALDNRLFAKMNGIGNEIVVVDLRDQPAPVTPDDARAVAAHVPYDQLMLLQPARLPGTEAFVRIYNNDGSESGACGNGMR
CVARQLFAGSDQQGLTFETRAGLLNCWRGPAEGLFTVDMGAPKFGWQDIPLAEEFRDTRGIELQIGPIDAPILHTPSVVS
MGNPHAIFWVDDIHAYDLGQFGPLLENHPIFPERANITLAHIVDRQHITMRTWERGAGLTRACGSAACATAVAAARLRRT
DRIVEMTLPGGKLTIEWREGDDHVLMTGGAELEFEGRFDPALFVGACDTTAA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=278, Percent_Identity=37.0503597122302, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_RHOP2 (Q2J390)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483981.1
- HSSP:   P44859
- ProteinModelPortal:   Q2J390
- SMR:   Q2J390
- STRING:   Q2J390
- GeneID:   3908625
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0359
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   RPAL316058:RPB_0359-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31862; Mature: 31730

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALDNRLFAKMNGIGNEIVVVDLRDQPAPVTPDDARAVAAHVPYDQLMLLQPARLPGTE
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCHHHEEEECCCCCCCCE
AFVRIYNNDGSESGACGNGMRCVARQLFAGSDQQGLTFETRAGLLNCWRGPAEGLFTVDM
EEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEHHHHCHHHHCCCCCCCEEEEEC
GAPKFGWQDIPLAEEFRDTRGIELQIGPIDAPILHTPSVVSMGNPHAIFWVDDIHAYDLG
CCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCEECCC
QFGPLLENHPIFPERANITLAHIVDRQHITMRTWERGAGLTRACGSAACATAVAAARLRR
CCCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEHHCCCCHHHHHCHHHHHHHHHHHHHHH
TDRIVEMTLPGGKLTIEWREGDDHVLMTGGAELEFEGRFDPALFVGACDTTAA
CCCEEEEEECCCEEEEEEECCCCEEEEECCCEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SALDNRLFAKMNGIGNEIVVVDLRDQPAPVTPDDARAVAAHVPYDQLMLLQPARLPGTE
CCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCHHHEEEECCCCCCCCE
AFVRIYNNDGSESGACGNGMRCVARQLFAGSDQQGLTFETRAGLLNCWRGPAEGLFTVDM
EEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEHHHHCHHHHCCCCCCCEEEEEC
GAPKFGWQDIPLAEEFRDTRGIELQIGPIDAPILHTPSVVSMGNPHAIFWVDDIHAYDLG
CCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCEECCC
QFGPLLENHPIFPERANITLAHIVDRQHITMRTWERGAGLTRACGSAACATAVAAARLRR
CCCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEHHCCCCHHHHHCHHHHHHHHHHHHHHH
TDRIVEMTLPGGKLTIEWREGDDHVLMTGGAELEFEGRFDPALFVGACDTTAA
CCCEEEEEECCCEEEEEEECCCCEEEEECCCEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA