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The map label for this gene is yleA [C]

Identifier: 86747486

GI number: 86747486

Start: 402018

End: 403286

Strand: Direct

Name: yleA [C]

Synonym: RPB_0360

Alternate gene names: 86747486

Gene position: 402018-403286 (Clockwise)

Preceding gene: 86747485

Following gene: 86747488

Centisome position: 7.54

GC content: 68.09

Gene sequence:

>1269_bases
ATGCCGGTCGAGATCGTCACCTTCGGCTGCCGCCTCAACGCCTTCGAATCCGAAGTGATCCGCCGCGAGGCGGAGGGCGC
CGGCCTTACCGAGACGATCGTCGTCAATAGCTGCGCCGTGACCAATGAGGCCGTGGCGCAGGCGCGGCAGTCGATCCGCA
AGCTCAAGCGCGCGCGGCCGGACGCGCGCATCGTCGTCACCGGCTGCGCGGCGCAGACCGAACCGGCGACCTTCGCCGCG
ATGGACGAGGTCGATCGCGTCATCGGCAATGACGACAAGATCCGCACGGAGGCCTGGCGCGAAGCGCGCGCGGCGTTCGA
CGCCGATGCGTTCGGGCTCGGCAATGAAGAGAAAATCGCGGTCGCCGACATCATGGCGGTGCGCGAGATGGCGCCGCATC
TCGTGGACGGTTACCAGAGCGGCCTGCCGCGGGTGTTCGTGCAGGTGCAGAACGGCTGCGACCATCGCTGCACCTTCTGC
ATCATCCCCTATGGTCGTGGTAACTCCCGCTCGGTGCCGGTCGGCGCGGTGGTCGATCAGGTCCGCGCGCTGGCCGAACG
CGGCCATGCCGAGATCGTGCTGACCGGCGTCGATCTCACCAGCTACGGCGCCGACCTGCCGGGCACACCGAAGCTCGGCA
CGCTGGTGAAGAAGGTGCTGCGCCACGTCCCCGAACTGCGGCGGCTGCGGATCTCCTCGATCGATTCCATCGAGGCCGAT
CGCGACCTGCTCGATGCGCTCGCCAGCGAAGAGCGATTGATGCCGCATCTGCATCTGTCGCTACAGGCCGGCGACGATCT
GATCCTGAAGCGGATGAAGCGGCGGCACGCGCGGCAGGATGCAATTGATTTCTGCGACGAGGTCCGCCGGCTGCGCCCCG
ACATCGCGCTCGGCGCCGATCTGATCGCGGGCTTCCCGACCGAGACCGACGCGATGTTCCAACGCTCACTCGATCTCGTC
GCGGAGTGCGGCCTGACCTTCCTCCACGTATTTCCCTATTCGAAACGCCCCGGCACCCCGGCCGCCCGGATGCCGCAGCT
CGACGGCCGCGTCGTGCGCCAACGGGCCGCGCAACTCCGCGCCGCCGGCGAGGCGGCGTTGCAGAAACGCCTTGCGGCGG
AGATCGGCGCCACCCGCGCCGTGCTGATCGAAAGTGCTGCGCAAGGCCGCACCGAGCATTTCATCCCGGTGGCGATTGCG
GGCGTGGCGCCGGGCGACGTGCGGACGATGGCGATCCGCGGGCACGATGGAGTACGACTGATTGTTTAG

Upstream 100 bases:

>100_bases
GCGACGATCACGTGCTGATGACCGGCGGCGCCGAGCTCGAATTCGAGGGCCGGTTCGATCCGGCGCTGTTCGTCGGCGCG
TGTGACACGACCGCCGCCTG

Downstream 100 bases:

>100_bases
CGCTTGTCATGCTGAGCGATGAGCCGCTGCGTCTCCCCGCATTCCCACGTCATCGCCCGGCTCGACCGGGCGACCCAGTA
TCCCAGAGCAGCCGTCTGTT

Product: MiaB-like tRNA modifying enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPVEIVTFGCRLNAFESEVIRREAEGAGLTETIVVNSCAVTNEAVAQARQSIRKLKRARPDARIVVTGCAAQTEPATFAA
MDEVDRVIGNDDKIRTEAWREARAAFDADAFGLGNEEKIAVADIMAVREMAPHLVDGYQSGLPRVFVQVQNGCDHRCTFC
IIPYGRGNSRSVPVGAVVDQVRALAERGHAEIVLTGVDLTSYGADLPGTPKLGTLVKKVLRHVPELRRLRISSIDSIEAD
RDLLDALASEERLMPHLHLSLQAGDDLILKRMKRRHARQDAIDFCDEVRRLRPDIALGADLIAGFPTETDAMFQRSLDLV
AECGLTFLHVFPYSKRPGTPAARMPQLDGRVVRQRAAQLRAAGEAALQKRLAAEIGATRAVLIESAAQGRTEHFIPVAIA
GVAPGDVRTMAIRGHDGVRLIV

Sequences:

>Translated_422_residues
MPVEIVTFGCRLNAFESEVIRREAEGAGLTETIVVNSCAVTNEAVAQARQSIRKLKRARPDARIVVTGCAAQTEPATFAA
MDEVDRVIGNDDKIRTEAWREARAAFDADAFGLGNEEKIAVADIMAVREMAPHLVDGYQSGLPRVFVQVQNGCDHRCTFC
IIPYGRGNSRSVPVGAVVDQVRALAERGHAEIVLTGVDLTSYGADLPGTPKLGTLVKKVLRHVPELRRLRISSIDSIEAD
RDLLDALASEERLMPHLHLSLQAGDDLILKRMKRRHARQDAIDFCDEVRRLRPDIALGADLIAGFPTETDAMFQRSLDLV
AECGLTFLHVFPYSKRPGTPAARMPQLDGRVVRQRAAQLRAAGEAALQKRLAAEIGATRAVLIESAAQGRTEHFIPVAIA
GVAPGDVRTMAIRGHDGVRLIV
>Mature_421_residues
PVEIVTFGCRLNAFESEVIRREAEGAGLTETIVVNSCAVTNEAVAQARQSIRKLKRARPDARIVVTGCAAQTEPATFAAM
DEVDRVIGNDDKIRTEAWREARAAFDADAFGLGNEEKIAVADIMAVREMAPHLVDGYQSGLPRVFVQVQNGCDHRCTFCI
IPYGRGNSRSVPVGAVVDQVRALAERGHAEIVLTGVDLTSYGADLPGTPKLGTLVKKVLRHVPELRRLRISSIDSIEADR
DLLDALASEERLMPHLHLSLQAGDDLILKRMKRRHARQDAIDFCDEVRRLRPDIALGADLIAGFPTETDAMFQRSLDLVA
ECGLTFLHVFPYSKRPGTPAARMPQLDGRVVRQRAAQLRAAGEAALQKRLAAEIGATRAVLIESAAQGRTEHFIPVAIAG
VAPGDVRTMAIRGHDGVRLIV

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MTTase N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=375, Percent_Identity=29.3333333333333, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI28872784, Length=458, Percent_Identity=22.9257641921397, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28872782, Length=458, Percent_Identity=22.9257641921397, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786882, Length=461, Percent_Identity=26.4642082429501, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=25, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17553146, Length=372, Percent_Identity=27.4193548387097, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71996771, Length=216, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=25.858123569794, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19922432, Length=417, Percent_Identity=25.8992805755396, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 45978; Mature: 45847

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVEIVTFGCRLNAFESEVIRREAEGAGLTETIVVNSCAVTNEAVAQARQSIRKLKRARP
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
DARIVVTGCAAQTEPATFAAMDEVDRVIGNDDKIRTEAWREARAAFDADAFGLGNEEKIA
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCHH
VADIMAVREMAPHLVDGYQSGLPRVFVQVQNGCDHRCTFCIIPYGRGNSRSVPVGAVVDQ
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
VRALAERGHAEIVLTGVDLTSYGADLPGTPKLGTLVKKVLRHVPELRRLRISSIDSIEAD
HHHHHHCCCCEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RDLLDALASEERLMPHLHLSLQAGDDLILKRMKRRHARQDAIDFCDEVRRLRPDIALGAD
HHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHH
LIAGFPTETDAMFQRSLDLVAECGLTFLHVFPYSKRPGTPAARMPQLDGRVVRQRAAQLR
HHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHH
AAGEAALQKRLAAEIGATRAVLIESAAQGRTEHFIPVAIAGVAPGDVRTMAIRGHDGVRL
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCEE
IV
EC
>Mature Secondary Structure 
PVEIVTFGCRLNAFESEVIRREAEGAGLTETIVVNSCAVTNEAVAQARQSIRKLKRARP
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
DARIVVTGCAAQTEPATFAAMDEVDRVIGNDDKIRTEAWREARAAFDADAFGLGNEEKIA
CCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCHH
VADIMAVREMAPHLVDGYQSGLPRVFVQVQNGCDHRCTFCIIPYGRGNSRSVPVGAVVDQ
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHH
VRALAERGHAEIVLTGVDLTSYGADLPGTPKLGTLVKKVLRHVPELRRLRISSIDSIEAD
HHHHHHCCCCEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RDLLDALASEERLMPHLHLSLQAGDDLILKRMKRRHARQDAIDFCDEVRRLRPDIALGAD
HHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHH
LIAGFPTETDAMFQRSLDLVAECGLTFLHVFPYSKRPGTPAARMPQLDGRVVRQRAAQLR
HHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHH
AAGEAALQKRLAAEIGATRAVLIESAAQGRTEHFIPVAIAGVAPGDVRTMAIRGHDGVRL
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCCCEE
IV
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]