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The map label for this gene is trmD

Identifier: 86747476

GI number: 86747476

Start: 393368

End: 394126

Strand: Reverse

Name: trmD

Synonym: RPB_0350

Alternate gene names: 86747476

Gene position: 394126-393368 (Counterclockwise)

Preceding gene: 86747477

Following gene: 86747475

Centisome position: 7.39

GC content: 71.01

Gene sequence:

>759_bases
ATGACCTGGCGCGCCACCGTCCTCACGCTGTTTCCGGAGATGTTTCCCGGGCCGCTCGGCGTCAGCCTGGCGGGGCGGGG
GCTGGCGGCCGGGCTGTGGGCGCTGGAGGCGCGCGACATCCGCGACTCGGCCACTGACCGACACCGTAGCGTCGACGACA
CCCCGGCCGGCGGCGGGCCCGGCATGGTGCTGCGCGCCGACGTGCTGGCGGCGGCGATCGACGCCGCCGATCCTGTGCCG
GGCCGTCCCCGGCTGGTGATGAGCCCGCGCGGTCGGCCATTGACCCAGGCGCGCGTCGCGGAACTGGCGGCTGGGCCCGG
CCCGCTGATCGTCTGCGGCCGGTTCGAGGGCATCGACCAGCGCGTGATCGACGCCCGGAACCTGGAGGAGGTCTCGATCG
GCGACTACGTGCTGTCCGGCGGCGAGATCGCCGCGCTGGCGCTGATCGATGCCTGCGTCCGGCTGCTGCCCGGGGTGATG
GGCAAGCTCGATTCCTCGGCCGAGGAGAGCTTTTCGGCCGGGTTGCTGGAATATCCGCAATACACAAGGCCGAAGAGTTT
CGAGGGCCGTCCGATCCCCGAGATCCTGACCTCGGGCGACCACGCCAAGGTCGCCGCCTGGCGGCAGGCCGAGGCCGAGG
CGCTGACCCGGGCGCGCCGGCCTGATTTGTGGGCCGCAAGATCTGTGGCAAGACCCGCCGCAAACGCCCCCGCAAAAGGC
GAATCGCAAAAAACGCCAAAAAACAAGACGGACGGGTGA

Upstream 100 bases:

>100_bases
TCATCGCCGGGCTTGACCCGGCGATCCATCTTCTTGCGAAGGCGATGGATGCCCGGATCAAGTCCGGGCATGACGTCCGA
ATTCGCTGCACGGCCTACAC

Downstream 100 bases:

>100_bases
CAAACCCCCAGGATTGCCGTATAGCACCGGCAAATCCGTGCAATGGCAGGACAGTGATCTTCGCGCAGCCCGCGCCGGGA
TGGTCGGGCGCGCCGCCGAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTWRATVLTLFPEMFPGPLGVSLAGRGLAAGLWALEARDIRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAADPVP
GRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQRVIDARNLEEVSIGDYVLSGGEIAALALIDACVRLLPGVM
GKLDSSAEESFSAGLLEYPQYTRPKSFEGRPIPEILTSGDHAKVAAWRQAEAEALTRARRPDLWAARSVARPAANAPAKG
ESQKTPKNKTDG

Sequences:

>Translated_252_residues
MTWRATVLTLFPEMFPGPLGVSLAGRGLAAGLWALEARDIRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAADPVP
GRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQRVIDARNLEEVSIGDYVLSGGEIAALALIDACVRLLPGVM
GKLDSSAEESFSAGLLEYPQYTRPKSFEGRPIPEILTSGDHAKVAAWRQAEAEALTRARRPDLWAARSVARPAANAPAKG
ESQKTPKNKTDG
>Mature_251_residues
TWRATVLTLFPEMFPGPLGVSLAGRGLAAGLWALEARDIRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAADPVPG
RPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQRVIDARNLEEVSIGDYVLSGGEIAALALIDACVRLLPGVMG
KLDSSAEESFSAGLLEYPQYTRPKSFEGRPIPEILTSGDHAKVAAWRQAEAEALTRARRPDLWAARSVARPAANAPAKGE
SQKTPKNKTDG

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=249, Percent_Identity=43.7751004016064, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RHOP2 (Q2J399)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483972.1
- ProteinModelPortal:   Q2J399
- SMR:   Q2J399
- STRING:   Q2J399
- GeneID:   3908616
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0350
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   RPAL316058:RPB_0350-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26648; Mature: 26516

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWRATVLTLFPEMFPGPLGVSLAGRGLAAGLWALEARDIRDSATDRHRSVDDTPAGGGP
CCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GMVLRADVLAAAIDAADPVPGRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQ
CCEEHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHH
RVIDARNLEEVSIGDYVLSGGEIAALALIDACVRLLPGVMGKLDSSAEESFSAGLLEYPQ
HHHHHCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCC
YTRPKSFEGRPIPEILTSGDHAKVAAWRQAEAEALTRARRPDLWAARSVARPAANAPAKG
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
ESQKTPKNKTDG
CCCCCCCCCCCC
>Mature Secondary Structure 
TWRATVLTLFPEMFPGPLGVSLAGRGLAAGLWALEARDIRDSATDRHRSVDDTPAGGGP
CCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GMVLRADVLAAAIDAADPVPGRPRLVMSPRGRPLTQARVAELAAGPGPLIVCGRFEGIDQ
CCEEHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHH
RVIDARNLEEVSIGDYVLSGGEIAALALIDACVRLLPGVMGKLDSSAEESFSAGLLEYPQ
HHHHHCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCC
YTRPKSFEGRPIPEILTSGDHAKVAAWRQAEAEALTRARRPDLWAARSVARPAANAPAKG
CCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
ESQKTPKNKTDG
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA