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The map label for this gene is leuD [H]

Identifier: 86747468

GI number: 86747468

Start: 387447

End: 388052

Strand: Reverse

Name: leuD [H]

Synonym: RPB_0342

Alternate gene names: 86747468

Gene position: 388052-387447 (Counterclockwise)

Preceding gene: 86747469

Following gene: 86747466

Centisome position: 7.28

GC content: 62.38

Gene sequence:

>606_bases
ATGGACAAGTTCACCACGCTGGAAGGCGTCGCGGCGCCGCTGAAGATCATCAATGTCGACACCGACATGATCATCCCCAA
GCAGTATCTGAAGACCATCAAGCGCACCGGCCTCGGCAAGGGCCTGTTCTCGGAACAGCGCTACCAGGACGACGGCAGCG
AGAACCCGGACTTCATCCTCAACAAGCCGGCCTATCGCAGCGCCAAGATCCTGGTCGCCGGCGACAATTTCGGCTGCGGC
TCGAGCCGCGAACACGCCCCCTGGGCGCTGCTGGATTTCGGCATCCGCTGCGTGATCTCGACCTCGTTCGGCGACATCTT
CTACAACAATTGCTTCAAGAACGGCGTGCTGCCGATCCGCGTGTCGCAGGACGATCTCGACAAGCTGTTCGACGACGCCG
AGCGCGGCTCCAACGCCACCGTGACCATCGACCTGCCGGCGCAGGAAATCCGCGGCCCGGACGGCGGCACGGTGAAGTTC
GAGATCGACCCGTTCCGCAAGCACTGCCTGATCAACGGCCTGGACGACATCGGGCTGACGCTCGAGAAGAAAGCCTCGAT
CGACAGCTACGAAGACAAGCTCAAGACCGAACGCGCCTGGGCGTGA

Upstream 100 bases:

>100_bases
CGAGTGGGCTGAGCCCCCGCGTCACCCAGCCTCCCGAAAGAATCGCCTTGGAACGCTGGGCCGGGCCGGCTAAAATCCGG
CCTCAATCTGGGAAAATCGC

Downstream 100 bases:

>100_bases
TTTATACCTCTGCCCGCGCGCGGGGAGAGGTCGACCGGCGGAGCGCAGCGAAGCCGGTCGGGTGAGGGGGCGTTTCCGCG
AGGCCGAGACTCAGTGCCCG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYQDDGSENPDFILNKPAYRSAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIRVSQDDLDKLFDDAERGSNATVTIDLPAQEIRGPDGGTVKF
EIDPFRKHCLINGLDDIGLTLEKKASIDSYEDKLKTERAWA

Sequences:

>Translated_201_residues
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYQDDGSENPDFILNKPAYRSAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIRVSQDDLDKLFDDAERGSNATVTIDLPAQEIRGPDGGTVKF
EIDPFRKHCLINGLDDIGLTLEKKASIDSYEDKLKTERAWA
>Mature_201_residues
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYQDDGSENPDFILNKPAYRSAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIRVSQDDLDKLFDDAERGSNATVTIDLPAQEIRGPDGGTVKF
EIDPFRKHCLINGLDDIGLTLEKKASIDSYEDKLKTERAWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=49.4845360824742, Blast_Score=202, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=59.5959595959596, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22355; Mature: 22355

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYQDDGSENPDFIL
CCCCCCCCCCCCCEEEEECCCCEECCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEE
NKPAYRSAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIR
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHEECCEEEEEECCCCHHHHHHHHCCCEEEEE
VSQDDLDKLFDDAERGSNATVTIDLPAQEIRGPDGGTVKFEIDPFRKHCLINGLDDIGLT
ECHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCEEEEEECHHHHHHHHCCCCCCCEE
LEKKASIDSYEDKLKTERAWA
EECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYQDDGSENPDFIL
CCCCCCCCCCCCCEEEEECCCCEECCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEE
NKPAYRSAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGVLPIR
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHEECCEEEEEECCCCHHHHHHHHCCCEEEEE
VSQDDLDKLFDDAERGSNATVTIDLPAQEIRGPDGGTVKFEIDPFRKHCLINGLDDIGLT
ECHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCCCEEEEEECHHHHHHHHCCCCCCCEE
LEKKASIDSYEDKLKTERAWA
EECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]