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The map label for this gene is cynT [H]

Identifier: 86747466

GI number: 86747466

Start: 385455

End: 386099

Strand: Reverse

Name: cynT [H]

Synonym: RPB_0340

Alternate gene names: 86747466

Gene position: 386099-385455 (Counterclockwise)

Preceding gene: 86747468

Following gene: 86747463

Centisome position: 7.24

GC content: 65.89

Gene sequence:

>645_bases
ATGACATCGTTTCCGAAAGCCCTGGTCGAGGGCTACGAGGCGTTCCGGACGCAGCGGCTGCCGACTGAGCAGACCCGCTA
CCGCGAACTCTCGGAGCGCGGTCAATCGCCGGAAGTGATGGTGATCGGCTGCTGCGATTCGCGCGTCTCGCCCGAGGTGA
TCTTCGACGCCGGCCCGGGCGAGATGTTCGTGGTCCGCAACGTCGCCAATCTGGTGCCGGTCTACGAGCCCGACGGCGGC
GCCCACGGCGTCTCCGCGGCTCTGGAATTCGCCGTGCAGGTGCTCAAGGTCAAGCACATCGTGGTGCTCGGCCACGCCCA
ATGCGGCGGTATCAAAGCCTTCACCGACAAGACCCCGCCGCTGACCGAGAGCGATTTCATCGGCCGCTGGATGGCGATGT
TCACCAAGCCCGGCGAGGTGGTCGAACAGCGCGACCACGAGACCATGCAGGAATTCCGCACCCGGATCGAGAAGGCCGCG
GTGCATCGCAGCCTCGAAAACCTGATGACGTTTCCCTGCATCCAGATCCAGGTCGCGCGCGGCAAGATCCAGCTCCACGG
AGCCTATTTCGGCGTCGCCGCCGGCGATCTGTTCGTGCTCGACCCCGACAGCAAGGACTTCCGCCCCGCCGTCCCGCGCG
GGTGA

Upstream 100 bases:

>100_bases
TGATTATGCGGGCGGGATCAGGCGCACGCGCATCCTGGTCGCTGCAGCGGGTTCCGCTTCCCTAGACGCGACGACACCAT
TCCCTGGAGAGACAGCGCCG

Downstream 100 bases:

>100_bases
GCGAGCCGCCCCGATCATGAGCGTCATCCTGAGGTGCGCGCCCTTGCGCGCCTCGAAGGATGGCCGCAAAGGCGGGGCTC
GCAGCCGCATCCTTCGAGGC

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSFPKALVEGYEAFRTQRLPTEQTRYRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPGEMFVVRNVANLVPVYEPDGG
AHGVSAALEFAVQVLKVKHIVVLGHAQCGGIKAFTDKTPPLTESDFIGRWMAMFTKPGEVVEQRDHETMQEFRTRIEKAA
VHRSLENLMTFPCIQIQVARGKIQLHGAYFGVAAGDLFVLDPDSKDFRPAVPRG

Sequences:

>Translated_214_residues
MTSFPKALVEGYEAFRTQRLPTEQTRYRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPGEMFVVRNVANLVPVYEPDGG
AHGVSAALEFAVQVLKVKHIVVLGHAQCGGIKAFTDKTPPLTESDFIGRWMAMFTKPGEVVEQRDHETMQEFRTRIEKAA
VHRSLENLMTFPCIQIQVARGKIQLHGAYFGVAAGDLFVLDPDSKDFRPAVPRG
>Mature_213_residues
TSFPKALVEGYEAFRTQRLPTEQTRYRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPGEMFVVRNVANLVPVYEPDGGA
HGVSAALEFAVQVLKVKHIVVLGHAQCGGIKAFTDKTPPLTESDFIGRWMAMFTKPGEVVEQRDHETMQEFRTRIEKAAV
HRSLENLMTFPCIQIQVARGKIQLHGAYFGVAAGDLFVLDPDSKDFRPAVPRG

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=210, Percent_Identity=31.4285714285714, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1786318, Length=194, Percent_Identity=30.4123711340206, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324292, Length=180, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23722; Mature: 23590

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFPKALVEGYEAFRTQRLPTEQTRYRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPG
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCC
EMFVVRNVANLVPVYEPDGGAHGVSAALEFAVQVLKVKHIVVLGHAQCGGIKAFTDKTPP
CEEEEECHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCCCCC
LTESDFIGRWMAMFTKPGEVVEQRDHETMQEFRTRIEKAAVHRSLENLMTFPCIQIQVAR
CCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
GKIQLHGAYFGVAAGDLFVLDPDSKDFRPAVPRG
CEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TSFPKALVEGYEAFRTQRLPTEQTRYRELSERGQSPEVMVIGCCDSRVSPEVIFDAGPG
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCC
EMFVVRNVANLVPVYEPDGGAHGVSAALEFAVQVLKVKHIVVLGHAQCGGIKAFTDKTPP
CEEEEECHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCCCCC
LTESDFIGRWMAMFTKPGEVVEQRDHETMQEFRTRIEKAAVHRSLENLMTFPCIQIQVAR
CCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
GKIQLHGAYFGVAAGDLFVLDPDSKDFRPAVPRG
CEEEEEEEEEEEECCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]