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The map label for this gene is leuB

Identifier: 86747461

GI number: 86747461

Start: 380508

End: 381620

Strand: Direct

Name: leuB

Synonym: RPB_0335

Alternate gene names: 86747461

Gene position: 380508-381620 (Clockwise)

Preceding gene: 86747459

Following gene: 86747464

Centisome position: 7.14

GC content: 66.4

Gene sequence:

>1113_bases
ATGGCGACGCATAAACTGCTGCTTCTTCCCGGCGACGGTATCGGCACCGAAGTGATGGCCGAGGTGTCGCGGCTGATCGA
CTGGCTGAACAAAGCCGGCATTGCCAGCTTCGAAACCGAGCACGGCCTGGTCGGCGGCGCCGCCTATGATGCCGACAAGG
TCGCGATCACCGACGCCACCATGGCGCTGGCGCAGGCCTCCGACGCGGTGATCTTCGGCGCGGTCGGCGGGCCGAAATGG
GACGCGGTGCCGTATGACGCCCGCCCCGAGGCCGGCCTGCTGCGGCTGCGCAAGGATCTGGCGCTGTTCGCCAATCTGCG
TCCGGCGGTGTGCTATCCGGCGCTCGCCGAGGCTTCCAGCCTGAAGCCCGAAGTGGTCGAGGGCCTCGACATCATGATCG
TCCGCGAACTCACCGGCGGCGTCTATTTCGGCGAGCCGAAGACCATCACCGATCTCGGCAACGGCCAGAAGCGCGCGATC
GACACGCAAGTTTACGACACCTACGAGATCGAGCGGATCGGCCGCGTCGCCTTCGACCTCGCCCGCAAGCGCCGCAACAA
GGTCACCTCGATGGAGAAGCGCAACGTCATGAAGACGGGCGTGCTCTGGAACGAGGTGATCACCCAGGTGCACGCGCGCG
AATACAAGGACGTCCAGCTCGAGCACCAGCTCGCCGATTCGGGCGGCATGAATCTCGTCAAATGGCCGAAGCAGTTCGAC
GTCATCGTCACCGACAATCTGTTCGGCGACATGCTGAGCGACATCGCCGCGATGCTGACCGGCTCGCTCGGCATGCTGCC
CTCGGCCTCGCTCGGCGCGGTCGACGACACCACCGGCAAGCGCAAGGCGATGTACGAGCCGGTGCACGGCTCGGCGCCCG
ACATCGCCGGCAAGGGCCTGGCCAATCCGGTGGCGATGCTGGCCTCGTTCGGCATGGCGCTGCGCTATTCGCTCGACATG
GGCGAGCTCGCCGACAAGCTCGACGAAGCCATCGCCGTGGTGCTGGCCCGCGGCCTGCGCACCGCCGACATCAAGAGCGA
AGGCTCCACCGTGGTGTCGACCAGCCAGATGGGCGAAGCCATCGTCCAGGAGATGCAGGCGCTGCACGGCTGA

Upstream 100 bases:

>100_bases
ATGCCCGGGCTTGACCCGGGCATCCATCATTTTCGCGAGAAGCTGGATTGCCGGGTCAAGCCCGGCAATGACGGTGTCGT
AGATCTGAGGACCCCAAATC

Downstream 100 bases:

>100_bases
GGAGCGCGCTCGAAGCGTGTGGGCACGGCGCAACAGCGCGCCTCTGCCCACCCTCCGGACTCAACGCCGCAAACGCCTTC
CGCGTCATCCTGAGGTGCCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MATHKLLLLPGDGIGTEVMAEVSRLIDWLNKAGIASFETEHGLVGGAAYDADKVAITDATMALAQASDAVIFGAVGGPKW
DAVPYDARPEAGLLRLRKDLALFANLRPAVCYPALAEASSLKPEVVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAI
DTQVYDTYEIERIGRVAFDLARKRRNKVTSMEKRNVMKTGVLWNEVITQVHAREYKDVQLEHQLADSGGMNLVKWPKQFD
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGAVDDTTGKRKAMYEPVHGSAPDIAGKGLANPVAMLASFGMALRYSLDM
GELADKLDEAIAVVLARGLRTADIKSEGSTVVSTSQMGEAIVQEMQALHG

Sequences:

>Translated_370_residues
MATHKLLLLPGDGIGTEVMAEVSRLIDWLNKAGIASFETEHGLVGGAAYDADKVAITDATMALAQASDAVIFGAVGGPKW
DAVPYDARPEAGLLRLRKDLALFANLRPAVCYPALAEASSLKPEVVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAI
DTQVYDTYEIERIGRVAFDLARKRRNKVTSMEKRNVMKTGVLWNEVITQVHAREYKDVQLEHQLADSGGMNLVKWPKQFD
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGAVDDTTGKRKAMYEPVHGSAPDIAGKGLANPVAMLASFGMALRYSLDM
GELADKLDEAIAVVLARGLRTADIKSEGSTVVSTSQMGEAIVQEMQALHG
>Mature_369_residues
ATHKLLLLPGDGIGTEVMAEVSRLIDWLNKAGIASFETEHGLVGGAAYDADKVAITDATMALAQASDAVIFGAVGGPKWD
AVPYDARPEAGLLRLRKDLALFANLRPAVCYPALAEASSLKPEVVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAID
TQVYDTYEIERIGRVAFDLARKRRNKVTSMEKRNVMKTGVLWNEVITQVHAREYKDVQLEHQLADSGGMNLVKWPKQFDV
IVTDNLFGDMLSDIAAMLTGSLGMLPSASLGAVDDTTGKRKAMYEPVHGSAPDIAGKGLANPVAMLASFGMALRYSLDMG
ELADKLDEAIAVVLARGLRTADIKSEGSTVVSTSQMGEAIVQEMQALHG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=312, Percent_Identity=34.9358974358974, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI28178816, Length=318, Percent_Identity=29.874213836478, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI4758582, Length=375, Percent_Identity=28.5333333333333, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI28178821, Length=318, Percent_Identity=29.874213836478, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=29.5522388059701, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=37.3333333333333, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=47.5274725274725, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1788101, Length=374, Percent_Identity=32.0855614973262, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787381, Length=410, Percent_Identity=24.1463414634146, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=30.0859598853868, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=370, Percent_Identity=29.7297297297297, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=29.6610169491525, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.1136363636364, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=372, Percent_Identity=38.9784946236559, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=33.6158192090396, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=29.0502793296089, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324709, Length=373, Percent_Identity=30.2949061662198, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=34.3373493975904, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=34.3373493975904, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24661184, Length=352, Percent_Identity=31.5340909090909, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078635, Length=361, Percent_Identity=26.5927977839335, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078633, Length=361, Percent_Identity=26.5927977839335, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24650122, Length=361, Percent_Identity=26.5927977839335, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078637, Length=361, Percent_Identity=26.5927977839335, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI161078639, Length=357, Percent_Identity=26.6106442577031, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=27.2988505747126, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=27.2988505747126, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130355, Length=355, Percent_Identity=26.7605633802817, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_RHOP2 (Q2J3B4)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483957.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q2J3B4
- SMR:   Q2J3B4
- STRING:   Q2J3B4
- GeneID:   3908716
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0335
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   RPAL316058:RPB_0335-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39707; Mature: 39576

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 97-97 BINDING 107-107 BINDING 135-135 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHKLLLLPGDGIGTEVMAEVSRLIDWLNKAGIASFETEHGLVGGAAYDADKVAITDAT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEEHHHH
MALAQASDAVIFGAVGGPKWDAVPYDARPEAGLLRLRKDLALFANLRPAVCYPALAEASS
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
LKPEVVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAIDTQVYDTYEIERIGRVAFDL
CCHHHHCCCCEEEEEECCCCEEECCCCEEECCCCCCHHEECHHHHHHHHHHHHHHHHHHH
ARKRRNKVTSMEKRNVMKTGVLWNEVITQVHAREYKDVQLEHQLADSGGMNLVKWPKQFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGAVDDTTGKRKAMYEPVHGSAPDIAGKGL
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ANPVAMLASFGMALRYSLDMGELADKLDEAIAVVLARGLRTADIKSEGSTVVSTSQMGEA
CHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHH
IVQEMQALHG
HHHHHHHHCC
>Mature Secondary Structure 
ATHKLLLLPGDGIGTEVMAEVSRLIDWLNKAGIASFETEHGLVGGAAYDADKVAITDAT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCEEEHHHH
MALAQASDAVIFGAVGGPKWDAVPYDARPEAGLLRLRKDLALFANLRPAVCYPALAEASS
HHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
LKPEVVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAIDTQVYDTYEIERIGRVAFDL
CCHHHHCCCCEEEEEECCCCEEECCCCEEECCCCCCHHEECHHHHHHHHHHHHHHHHHHH
ARKRRNKVTSMEKRNVMKTGVLWNEVITQVHAREYKDVQLEHQLADSGGMNLVKWPKQFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGAVDDTTGKRKAMYEPVHGSAPDIAGKGL
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ANPVAMLASFGMALRYSLDMGELADKLDEAIAVVLARGLRTADIKSEGSTVVSTSQMGEA
CHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHH
IVQEMQALHG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA