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The map label for this gene is pat [H]

Identifier: 86747414

GI number: 86747414

Start: 326291

End: 326908

Strand: Direct

Name: pat [H]

Synonym: RPB_0288

Alternate gene names: 86747414

Gene position: 326291-326908 (Clockwise)

Preceding gene: 86747413

Following gene: 86747418

Centisome position: 6.12

GC content: 68.61

Gene sequence:

>618_bases
ATGGATACGCGGGTCAAGCCCGCGTATGACGCTGGGATTGGCGATCAGCCACGAGTTGAATTTCGCATGTCCGACCTCCT
GATCCGCCCGGCCGCCGCCGCCGACCTGCCCGCCATCACCGCGATCTACGCCGACGCGGTGCGCACCGGCACCGCGACCT
TCGAACTGACGCCGCCGGATCTCGCCGAAATGACGCGGCGGTTCGAAACGCTCGCCGGCGGCGGCTTTCCCTATCTGGTC
GCGCTGCTTGGCGACCGCGTCGCCGGCTACGCCTATGCGGGACCGTACCGGCCGCGTCCGGCCTATCGCTTCACCGTGGA
GAACTCGGTCTATCTCGATCCGCTCGCGCATCGCCGCGGCCTCGGCACCGCGCTGCTGCACGAGCTGATCGCGCAATGCG
AGGCGCGCGGCTTCCGCCAGATGATCGCGGTGATCGGCGATTCCACCAATGCCGGCTCGGTCGCCGTGCATCGCAAGAGC
GGCTTCGACCTGATCGGCGTCCACCGCAATGTCGGATTCAAATTCGGCCGCTGGCTCGACACCGTGATGATGCAACGCGC
GCTCGGCGCGGGCGGCGAGACGGTGCCGGAGGTGGGAGCGGGGCCGAGCAACGCGTGA

Upstream 100 bases:

>100_bases
CAGGATGCGAAGGCAGATGCTCCAACACGGTCGTCATCGCCGGGCTTGACCCGGCGATCCATCCTCTGGAGAGAACCTGT
CATGCCGCCTTGCGAAGGGG

Downstream 100 bases:

>100_bases
TGTCGTCACCCTCCGCGAAAGCGGAGGGCCCAATATTGCAGAGCGCTGGCGATTGGATCGCCGACGCTCCGGGGTACTGG
GTCGCCCGGTCGAGCCGGGC

Product: GCN5-related N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MDTRVKPAYDAGIGDQPRVEFRMSDLLIRPAAAADLPAITAIYADAVRTGTATFELTPPDLAEMTRRFETLAGGGFPYLV
ALLGDRVAGYAYAGPYRPRPAYRFTVENSVYLDPLAHRRGLGTALLHELIAQCEARGFRQMIAVIGDSTNAGSVAVHRKS
GFDLIGVHRNVGFKFGRWLDTVMMQRALGAGGETVPEVGAGPSNA

Sequences:

>Translated_205_residues
MDTRVKPAYDAGIGDQPRVEFRMSDLLIRPAAAADLPAITAIYADAVRTGTATFELTPPDLAEMTRRFETLAGGGFPYLV
ALLGDRVAGYAYAGPYRPRPAYRFTVENSVYLDPLAHRRGLGTALLHELIAQCEARGFRQMIAVIGDSTNAGSVAVHRKS
GFDLIGVHRNVGFKFGRWLDTVMMQRALGAGGETVPEVGAGPSNA
>Mature_205_residues
MDTRVKPAYDAGIGDQPRVEFRMSDLLIRPAAAADLPAITAIYADAVRTGTATFELTPPDLAEMTRRFETLAGGGFPYLV
ALLGDRVAGYAYAGPYRPRPAYRFTVENSVYLDPLAHRRGLGTALLHELIAQCEARGFRQMIAVIGDSTNAGSVAVHRKS
GFDLIGVHRNVGFKFGRWLDTVMMQRALGAGGETVPEVGAGPSNA

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=161, Percent_Identity=38.5093167701863, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 22039; Mature: 22039

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTRVKPAYDAGIGDQPRVEFRMSDLLIRPAAAADLPAITAIYADAVRTGTATFELTPPD
CCCCCCCHHCCCCCCCCCEEEEHHHHEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
LAEMTRRFETLAGGGFPYLVALLGDRVAGYAYAGPYRPRPAYRFTVENSVYLDPLAHRRG
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCEEECCHHHHCC
LGTALLHELIAQCEARGFRQMIAVIGDSTNAGSVAVHRKSGFDLIGVHRNVGFKFGRWLD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHH
TVMMQRALGAGGETVPEVGAGPSNA
HHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDTRVKPAYDAGIGDQPRVEFRMSDLLIRPAAAADLPAITAIYADAVRTGTATFELTPPD
CCCCCCCHHCCCCCCCCCEEEEHHHHEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
LAEMTRRFETLAGGGFPYLVALLGDRVAGYAYAGPYRPRPAYRFTVENSVYLDPLAHRRG
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCEEECCHHHHCC
LGTALLHELIAQCEARGFRQMIAVIGDSTNAGSVAVHRKSGFDLIGVHRNVGFKFGRWLD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHH
TVMMQRALGAGGETVPEVGAGPSNA
HHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA