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The map label for this gene is phaE [H]

Identifier: 86747278

GI number: 86747278

Start: 165444

End: 165932

Strand: Direct

Name: phaE [H]

Synonym: RPB_0151

Alternate gene names: 86747278

Gene position: 165444-165932 (Clockwise)

Preceding gene: 86747277

Following gene: 86747279

Centisome position: 3.1

GC content: 65.03

Gene sequence:

>489_bases
ATGACCAGGATGTTTCCGCATCCGATCCTCAGCCTGGTGATCGCCGGCGTGTTCGTCGGCCTGATCAACACGCTGTCGCT
CGGGAGCGTGGTGCTGGGCCTGCTGCTCGGCATCGCGATCCCGTGGTTCACCAGCCCGTATTGGCCGAATCGGCCGACGG
TCCGCAGCCCGTTGAAGCTCGCCGCCTATCTGGCGATCGTCGGCTGGGACATCGTCGTGTCCAACGTCCAGGTCGCCTAC
TGGGTGCTGTTCCGGCGCAGCGCGAGCCTGCAATCGCAATTCGTCGTGGTGCCGCTGGAGCTGACCTCACCCGAGGCGAT
CGCGCTGCTGGCGGGGACGATCACGATGACGCCGGGGACGGTCAGCGCCGATCTCGCCGCCGACGGCAGCGCCATTCTGG
TGCATTGCCTGCACACCACCGATCCCGTCGACACGGTCGAGCAGATCAAGACTCGCTACGAACGACGACTGAAGGAGATC
TTCGGATGA

Upstream 100 bases:

>100_bases
GCCGCGAACTGGCGGCGACGGCGCAACAGACGCTCGACATCAAGGCCTATGTCCGCGCGGTGAACCCCGCCGACCTGGCA
CTGTCGCGGGGGGCGCCGCA

Downstream 100 bases:

>100_bases
TCGCAACAGCGTGCCTCATCGCCGTCGGGGTGATCAGCGTCTCGGCGCTGATGAACCTCTTCCGGCTCGCCACCGGGCCC
GACGTGCTCGACCGGATCCT

Product: putative monovalent cation/H+ antiporter subunit E

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein E; Pha system subunit E [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MTRMFPHPILSLVIAGVFVGLINTLSLGSVVLGLLLGIAIPWFTSPYWPNRPTVRSPLKLAAYLAIVGWDIVVSNVQVAY
WVLFRRSASLQSQFVVVPLELTSPEAIALLAGTITMTPGTVSADLAADGSAILVHCLHTTDPVDTVEQIKTRYERRLKEI
FG

Sequences:

>Translated_162_residues
MTRMFPHPILSLVIAGVFVGLINTLSLGSVVLGLLLGIAIPWFTSPYWPNRPTVRSPLKLAAYLAIVGWDIVVSNVQVAY
WVLFRRSASLQSQFVVVPLELTSPEAIALLAGTITMTPGTVSADLAADGSAILVHCLHTTDPVDTVEQIKTRYERRLKEI
FG
>Mature_161_residues
TRMFPHPILSLVIAGVFVGLINTLSLGSVVLGLLLGIAIPWFTSPYWPNRPTVRSPLKLAAYLAIVGWDIVVSNVQVAYW
VLFRRSASLQSQFVVVPLELTSPEAIALLAGTITMTPGTVSADLAADGSAILVHCLHTTDPVDTVEQIKTRYERRLKEIF
G

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004847
- InterPro:   IPR002758 [H]

Pfam domain/function: PF01899 MNHE [H]

EC number: NA

Molecular weight: Translated: 17641; Mature: 17510

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRMFPHPILSLVIAGVFVGLINTLSLGSVVLGLLLGIAIPWFTSPYWPNRPTVRSPLKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
AAYLAIVGWDIVVSNVQVAYWVLFRRSASLQSQFVVVPLELTSPEAIALLAGTITMTPGT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEHEEEEEEECCCC
VSADLAADGSAILVHCLHTTDPVDTVEQIKTRYERRLKEIFG
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRMFPHPILSLVIAGVFVGLINTLSLGSVVLGLLLGIAIPWFTSPYWPNRPTVRSPLKL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
AAYLAIVGWDIVVSNVQVAYWVLFRRSASLQSQFVVVPLELTSPEAIALLAGTITMTPGT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEHEEEEEEECCCC
VSADLAADGSAILVHCLHTTDPVDTVEQIKTRYERRLKEIFG
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]