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The map label for this gene is gyrB [H]

Identifier: 86747132

GI number: 86747132

Start: 5462

End: 7903

Strand: Direct

Name: gyrB [H]

Synonym: RPB_0005

Alternate gene names: 86747132

Gene position: 5462-7903 (Clockwise)

Preceding gene: 86747131

Following gene: 86747148

Centisome position: 0.1

GC content: 66.01

Gene sequence:

>2442_bases
ATGACTGAACCCGCCCGGCAGCCGATCGCCGAAACCCCGTCGCCCGTTCCGGCCGAATACGGCGCGGAATCGATCCGGGT
GCTGAAGGGCCTCGACGCTGTCCGCAAGCGGCCGGGCATGTATATCGGCGACACCGACGACGGCTCCGGCCTGCACCACA
TGGTGTACGAAGTCGTCGACAACGCCATCGACGAAGCGCTCGCCGGCCACGCCACCGCGGTCGAAGTCGTGCTCAACGCC
GACGGCTCGGTGACGGTGCGCGACGACGGCCGCGGCATTCCGGTCGACATCCACAAGGGCGAAGGCATTTCGGCGGCCGA
AGTCATCATGACCCAGCTCCATGCCGGCGGAAAATTCGACCAGAACTCCTACAAGGTGTCCGGCGGACTGCACGGCGTCG
GCGTCTCGGTGGTCAATGCGCTGTCGAGCAAGCTGGTCCTGCGCGTGTGGCGTAACGGCAAAGAGCACATCATCGAATTC
GCCCATGGCGACGCGGTGGCGCCGCTGAGCGTTGTCGGCGACGCCAATGGCCGGCGCGGCACCGAGGTGACATTTTTCGC
GTCGCCGGAAACCTTCACCCACATCGAATACGACTTCGCGACGCTGGAGCATCGCTTACGCGAACTCGCCTTCCTGAATT
CCGGCGTGAACATCGTATTGTCCGACATGCGCCACCCGGTCGAGAAGCGCGAGGAGATGCGCTACGAGGGCGGCGTCACC
GAATTCGTCAAATATCTCGACCGCAACAAGAAGCCGATGGTGCCGGCGCCGATCGTGCTGGCGGCGGATCTCAACGGCAT
CGGCGTCGAAGCCGCATTGTGGTGGAACGAGACCTACCACGAGAACGTGCTGTGCTTCACCAACAACATCCCGCAGCGCG
ACGGTGGTACGCATCTGGCCGGCTTCCGCGCCGCGCTGACGCGGCAGGTCAACGGCTATGCGGACAGCAACGCCAAGAAG
GAAAAGATCGCGCTGACCGGCGACGATTGCCGCGAAGGCCTCACCGCGGTGCTGTCGGTGAAGGTGCCGGATCCGAAGTT
CTCGTCGCAGACCAAGGACAAGCTGGTGTCCTCGGAAGTCCGGCCGGTGGTCGAGAACGTGCTGAACGAAGCGCTCGCAG
CCTGGTTCGAGGAACATCCCGCCGAAGCCAAGGTCATCGTCGGCAAGGTGATCGAAGCCGCCGCCGCCCGCGAAGCCGCG
CGAAAAGCGCGCGAGCTGACGCGGCGCAAGGGCGCGCTGGATATCGCCTCGCTGCCCGGTAAGCTCGCCGACTGCCAGGA
GCGCGATCCGTCGAAATCCGAACTGTTCATCGTCGAGGGTGACTCCGCCGGCGGCTCCGCCAAGCAGGGCCGCAACCGCG
AATTCCAGGCCGTGCTGCCGCTGCGCGGCAAGATCCTCAATGTCGAGCGCGCGCGCTTCGACAAGATGCTGGGCTCCGAG
CAGATCGGCACGCTGATCACCGCGCTCGGCACCGGCATCGGCCGCGACGATTTCGACATCGCCAAGCTGCGCTACCACAA
GATCATCCTGATGACCGACGCCGACGTCGACGGCGCGCACATCCGCACGCTGCTCTTGACGTTCTTCTTCCGGCAGATGC
CGGCTCTGATCGAGGGCGGCTTCCTCTACATCGCGCAGCCGCCGCTGTACAAAGTGACGCGCGGCAAGTCGGAGCAGTAT
CTCAAGGACGAGCGCGCGCTCGAGGAATATCTGATCGCGACCGGACTCGACGATTGCGCGTTCAAGATCGCCTCCGGCGA
AGTCCGCGGCGGGCGGGATCTGCTGGCGCTGGTCGAGGACGCCCGCGTGATCCGCCAGACGCTGCACAATCTGCACGGCC
GCTACAATCGCTCGGTGGTCGAGCAGGCGGCGATCGCCGGCGTGCTCAGCCCGCGGATCACCAGCGACGTGGCGACCGCC
AACGAAGCCGCCGCCTATATCGCGCGCCGGCTCGACGCGCTGGCCGACGAGGTCGAGCGCGGCTGGATCGGCCGCTTCGT
CGAAGGCGAAGGCTTCCACTTCGAGCGCACCGTGCGCGGCGTCAAGGACGTGGCGATGATCGACGACGCGCTGCTCGGCT
CCGCCGACGCCCGCAAGCTCGATGAATACGCCGCCGTGCTGCAGGAGGTCTATCCGCGCCCCGGCGTGCTGCGCCGCAAG
GATGCGGAAACCGCGATCCACGGCCCGGTCAGCCTGTTCGAGGCGGTGACCGACGCTGGCCGCAAGGGCGTGGCGCTGCA
GCGCTACAAAGGCCTCGGCGAGATGAACCCGAGCCAGCTCTGGGAGACCACGCTCGACACCAACGCCCGCTCGCTGCTGC
AGGTGAAGGTCCGCGAAGTCGACGAAGCCGACGACATCTTCACCAAGCTGATGGGCGACGTCGTCGAACCCCGCCGCGAA
TTCATCCAGGAGAATTCGCTGAGCGCGACGGTGGACGTGTAA

Upstream 100 bases:

>100_bases
TGCCACTTTGCGCTAGGCGCAATCCGTCTTTCGTGGCACAAAAGGCGCTGCATGCGCGCCCTCGCAAGCCGATTCGAACT
CATCCTTCAAAGGTTCCTGC

Downstream 100 bases:

>100_bases
ACCCACTGCAGCGGCGCCAGCCTACTCCGCCCGGCTGACGATCACGCCGTACCAGCCGAGGCCCCGATAGGTTTCGTAGC
CCGGGGTGAGGTGGAAGCCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MTEPARQPIAETPSPVPAEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATAVEVVLNA
DGSVTVRDDGRGIPVDIHKGEGISAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLVLRVWRNGKEHIIEF
AHGDAVAPLSVVGDANGRRGTEVTFFASPETFTHIEYDFATLEHRLRELAFLNSGVNIVLSDMRHPVEKREEMRYEGGVT
EFVKYLDRNKKPMVPAPIVLAADLNGIGVEAALWWNETYHENVLCFTNNIPQRDGGTHLAGFRAALTRQVNGYADSNAKK
EKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVLNEALAAWFEEHPAEAKVIVGKVIEAAAAREAA
RKARELTRRKGALDIASLPGKLADCQERDPSKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFDKMLGSE
QIGTLITALGTGIGRDDFDIAKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPALIEGGFLYIAQPPLYKVTRGKSEQY
LKDERALEEYLIATGLDDCAFKIASGEVRGGRDLLALVEDARVIRQTLHNLHGRYNRSVVEQAAIAGVLSPRITSDVATA
NEAAAYIARRLDALADEVERGWIGRFVEGEGFHFERTVRGVKDVAMIDDALLGSADARKLDEYAAVLQEVYPRPGVLRRK
DAETAIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPSQLWETTLDTNARSLLQVKVREVDEADDIFTKLMGDVVEPRRE
FIQENSLSATVDV

Sequences:

>Translated_813_residues
MTEPARQPIAETPSPVPAEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATAVEVVLNA
DGSVTVRDDGRGIPVDIHKGEGISAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLVLRVWRNGKEHIIEF
AHGDAVAPLSVVGDANGRRGTEVTFFASPETFTHIEYDFATLEHRLRELAFLNSGVNIVLSDMRHPVEKREEMRYEGGVT
EFVKYLDRNKKPMVPAPIVLAADLNGIGVEAALWWNETYHENVLCFTNNIPQRDGGTHLAGFRAALTRQVNGYADSNAKK
EKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVLNEALAAWFEEHPAEAKVIVGKVIEAAAAREAA
RKARELTRRKGALDIASLPGKLADCQERDPSKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFDKMLGSE
QIGTLITALGTGIGRDDFDIAKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPALIEGGFLYIAQPPLYKVTRGKSEQY
LKDERALEEYLIATGLDDCAFKIASGEVRGGRDLLALVEDARVIRQTLHNLHGRYNRSVVEQAAIAGVLSPRITSDVATA
NEAAAYIARRLDALADEVERGWIGRFVEGEGFHFERTVRGVKDVAMIDDALLGSADARKLDEYAAVLQEVYPRPGVLRRK
DAETAIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPSQLWETTLDTNARSLLQVKVREVDEADDIFTKLMGDVVEPRRE
FIQENSLSATVDV
>Mature_812_residues
TEPARQPIAETPSPVPAEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATAVEVVLNAD
GSVTVRDDGRGIPVDIHKGEGISAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSKLVLRVWRNGKEHIIEFA
HGDAVAPLSVVGDANGRRGTEVTFFASPETFTHIEYDFATLEHRLRELAFLNSGVNIVLSDMRHPVEKREEMRYEGGVTE
FVKYLDRNKKPMVPAPIVLAADLNGIGVEAALWWNETYHENVLCFTNNIPQRDGGTHLAGFRAALTRQVNGYADSNAKKE
KIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVENVLNEALAAWFEEHPAEAKVIVGKVIEAAAAREAAR
KARELTRRKGALDIASLPGKLADCQERDPSKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFDKMLGSEQ
IGTLITALGTGIGRDDFDIAKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPALIEGGFLYIAQPPLYKVTRGKSEQYL
KDERALEEYLIATGLDDCAFKIASGEVRGGRDLLALVEDARVIRQTLHNLHGRYNRSVVEQAAIAGVLSPRITSDVATAN
EAAAYIARRLDALADEVERGWIGRFVEGEGFHFERTVRGVKDVAMIDDALLGSADARKLDEYAAVLQEVYPRPGVLRRKD
AETAIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPSQLWETTLDTNARSLLQVKVREVDEADDIFTKLMGDVVEPRREF
IQENSLSATVDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=549, Percent_Identity=24.2258652094718, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI19913406, Length=565, Percent_Identity=24.2477876106195, Blast_Score=128, Evalue=3e-29,
Organism=Escherichia coli, GI48994957, Length=811, Percent_Identity=55.4870530209618, Blast_Score=848, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=546, Percent_Identity=41.025641025641, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17535065, Length=540, Percent_Identity=24.4444444444444, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=516, Percent_Identity=23.062015503876, Blast_Score=109, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=143, Percent_Identity=33.5664335664336, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=598, Percent_Identity=24.4147157190635, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17136538, Length=542, Percent_Identity=25.2767527675277, Blast_Score=133, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89024; Mature: 88893

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPARQPIAETPSPVPAEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVD
CCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHH
NAIDEALAGHATAVEVVLNADGSVTVRDDGRGIPVDIHKGEGISAAEVIMTQLHAGGKFD
HHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCC
QNSYKVSGGLHGVGVSVVNALSSKLVLRVWRNGKEHIIEFAHGDAVAPLSVVGDANGRRG
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCCCCEEEECCCCCCC
TEVTFFASPETFTHIEYDFATLEHRLRELAFLNSGVNIVLSDMRHPVEKREEMRYEGGVT
CEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHCCCHH
EFVKYLDRNKKPMVPAPIVLAADLNGIGVEAALWWNETYHENVLCFTNNIPQRDGGTHLA
HHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCHH
GFRAALTRQVNGYADSNAKKEKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEV
HHHHHHHHHHCCCCCCCCCCCEEEECCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHH
RPVVENVLNEALAAWFEEHPAEAKVIVGKVIEAAAAREAARKARELTRRKGALDIASLPG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
KLADCQERDPSKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFDKMLGSE
CHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCHH
QIGTLITALGTGIGRDDFDIAKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPALIEGG
HHHHHHHHHHCCCCCCCCHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
FLYIAQPPLYKVTRGKSEQYLKDERALEEYLIATGLDDCAFKIASGEVRGGRDLLALVED
EEEEECCCCEEHHCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCHHHHHHHHH
ARVIRQTLHNLHGRYNRSVVEQAAIAGVLSPRITSDVATANEAAAYIARRLDALADEVER
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWIGRFVEGEGFHFERTVRGVKDVAMIDDALLGSADARKLDEYAAVLQEVYPRPGVLRRK
CHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DAETAIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPSQLWETTLDTNARSLLQVKVREV
CCCHHHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
DEADDIFTKLMGDVVEPRREFIQENSLSATVDV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure 
TEPARQPIAETPSPVPAEYGAESIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVD
CCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHH
NAIDEALAGHATAVEVVLNADGSVTVRDDGRGIPVDIHKGEGISAAEVIMTQLHAGGKFD
HHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCC
QNSYKVSGGLHGVGVSVVNALSSKLVLRVWRNGKEHIIEFAHGDAVAPLSVVGDANGRRG
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCCCCEEEECCCCCCC
TEVTFFASPETFTHIEYDFATLEHRLRELAFLNSGVNIVLSDMRHPVEKREEMRYEGGVT
CEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHCCCHH
EFVKYLDRNKKPMVPAPIVLAADLNGIGVEAALWWNETYHENVLCFTNNIPQRDGGTHLA
HHHHHHHCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCHH
GFRAALTRQVNGYADSNAKKEKIALTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEV
HHHHHHHHHHCCCCCCCCCCCEEEECCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHH
RPVVENVLNEALAAWFEEHPAEAKVIVGKVIEAAAAREAARKARELTRRKGALDIASLPG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
KLADCQERDPSKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFDKMLGSE
CHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCHH
QIGTLITALGTGIGRDDFDIAKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPALIEGG
HHHHHHHHHHCCCCCCCCHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
FLYIAQPPLYKVTRGKSEQYLKDERALEEYLIATGLDDCAFKIASGEVRGGRDLLALVED
EEEEECCCCEEHHCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCHHHHHHHHH
ARVIRQTLHNLHGRYNRSVVEQAAIAGVLSPRITSDVATANEAAAYIARRLDALADEVER
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWIGRFVEGEGFHFERTVRGVKDVAMIDDALLGSADARKLDEYAAVLQEVYPRPGVLRRK
CHHCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DAETAIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPSQLWETTLDTNARSLLQVKVREV
CCCHHHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
DEADDIFTKLMGDVVEPRREFIQENSLSATVDV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]