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The map label for this gene is recF [H]

Identifier: 86747131

GI number: 86747131

Start: 4098

End: 5234

Strand: Direct

Name: recF [H]

Synonym: RPB_0004

Alternate gene names: 86747131

Gene position: 4098-5234 (Clockwise)

Preceding gene: 86747130

Following gene: 86747132

Centisome position: 0.08

GC content: 71.5

Gene sequence:

>1137_bases
ATGACCGCCTCCCGCATCACCCGGCTGACGTTGACGCATTTCCGCAATTATCGGGCGGCGGTGCTGACGACGAGTGCCGA
GCGCGTGGTGCTGGTCGGCGCCAACGGCGCCGGCAAGACCAATTGCCTCGAGGCGATCTCGTTTCTGTCGCCGGGGCGGG
GGTTGCGGCGGGCGACGCTGGACGACGTCGCCGACAATGAGGGCGACGGCTCCTGGGCGGTGGCGGCGGAGGTCGAGGGC
GCGCTCGGGCTGGCGACGCTCGGCACCGGGATCGATCCGCCGCGCGCCGACGCCGCGACCTCGCGGCGCTGCCGGATCGA
CCGCGAGCCGGTCGGCTCCGCCACCGCATTCGGCGATCACTTACGCATGGTGTGGCTGACGCCGGCGATGGACGGGCTGT
TCATGGGCGCGGCCTCGGAACGGCGGCGGTTCTTCGACCGGCTGGTGCTGGCGATCGACAGCCAGCATTCGGGCCGGGTC
TCGGCGCTGGACCGCAGCCTCAGATCGCGGAACCGCCTGCTGGAGGTACGTTACCCCGACGCGCATTGGCTCGATGCGAT
CGAGCGCGAAACCGCCGAGCTCGCGGTCGCGGTCGCGGCGATGCGCGGCCAGACCGCGATGCGCCTCGCCGCGATGCTCG
ACGCCCGCGGCGCGGCATCGGCGTTTCCGTCGGCGAAGATCATGCTCGACGGCTGGATGGAGAGCGCGCTGCTCACCGAA
CCCGCCACGGCGGTGGAAGATCGCTACCGCACCATCCTGCGCGAGGGCCGCCCGCGCGACGCCGCCGCCGGCCGCACCCT
CGACGGCCCGCATCTGACCGACCTCGAAGTCGTCTACGCGCCGAAGGCGATGCCGGCGCGCGACGCCTCCACCGGCGAAC
AGAAGGCGCTGCTGATCGGGCTCGTCCTCGCGCATGCGCAGCTCGTCTCGGAGATGACCGGCATCACGCCGCTGCTGCTG
CTCGACGAGGTGGTGGCGCATCTCGACCCGTCGCGGCGCGCCGCGCTGTTCGAGGAATTGGCGAAGCTCGGCGCCCAGGT
CTGGATGACCGGCGCCGACCCCGCAGCGTTCGCCGAGATCGGTTCCGGCGCCGAGATATTCACCGTCGAATCCGGCCGGA
TCAGGCCGCAACAATGA

Upstream 100 bases:

>100_bases
AAACGGACGAATAACCCCTCACCCGGCGCCTCACTTCGTTCGGCGCCACCCTCTCCCACAAGGGGAGAGGGTCACTCGGC
GCTGTTCTCGCTTTAATCAC

Downstream 100 bases:

>100_bases
GCGGCGGAGATCGCCCCGAAGGGGCCTCAACCGGCCCTTGCGAGCCGACATTCCCGAGTGCTCGAATCGGCCGTTTTCGG
GCTTGCTGAACCGATAAAAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTASRITRLTLTHFRNYRAAVLTTSAERVVLVGANGAGKTNCLEAISFLSPGRGLRRATLDDVADNEGDGSWAVAAEVEG
ALGLATLGTGIDPPRADAATSRRCRIDREPVGSATAFGDHLRMVWLTPAMDGLFMGAASERRRFFDRLVLAIDSQHSGRV
SALDRSLRSRNRLLEVRYPDAHWLDAIERETAELAVAVAAMRGQTAMRLAAMLDARGAASAFPSAKIMLDGWMESALLTE
PATAVEDRYRTILREGRPRDAAAGRTLDGPHLTDLEVVYAPKAMPARDASTGEQKALLIGLVLAHAQLVSEMTGITPLLL
LDEVVAHLDPSRRAALFEELAKLGAQVWMTGADPAAFAEIGSGAEIFTVESGRIRPQQ

Sequences:

>Translated_378_residues
MTASRITRLTLTHFRNYRAAVLTTSAERVVLVGANGAGKTNCLEAISFLSPGRGLRRATLDDVADNEGDGSWAVAAEVEG
ALGLATLGTGIDPPRADAATSRRCRIDREPVGSATAFGDHLRMVWLTPAMDGLFMGAASERRRFFDRLVLAIDSQHSGRV
SALDRSLRSRNRLLEVRYPDAHWLDAIERETAELAVAVAAMRGQTAMRLAAMLDARGAASAFPSAKIMLDGWMESALLTE
PATAVEDRYRTILREGRPRDAAAGRTLDGPHLTDLEVVYAPKAMPARDASTGEQKALLIGLVLAHAQLVSEMTGITPLLL
LDEVVAHLDPSRRAALFEELAKLGAQVWMTGADPAAFAEIGSGAEIFTVESGRIRPQQ
>Mature_377_residues
TASRITRLTLTHFRNYRAAVLTTSAERVVLVGANGAGKTNCLEAISFLSPGRGLRRATLDDVADNEGDGSWAVAAEVEGA
LGLATLGTGIDPPRADAATSRRCRIDREPVGSATAFGDHLRMVWLTPAMDGLFMGAASERRRFFDRLVLAIDSQHSGRVS
ALDRSLRSRNRLLEVRYPDAHWLDAIERETAELAVAVAAMRGQTAMRLAAMLDARGAASAFPSAKIMLDGWMESALLTEP
ATAVEDRYRTILREGRPRDAAAGRTLDGPHLTDLEVVYAPKAMPARDASTGEQKALLIGLVLAHAQLVSEMTGITPLLLL
DEVVAHLDPSRRAALFEELAKLGAQVWMTGADPAAFAEIGSGAEIFTVESGRIRPQQ

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=381, Percent_Identity=27.0341207349081, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40634; Mature: 40503

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASRITRLTLTHFRNYRAAVLTTSAERVVLVGANGAGKTNCLEAISFLSPGRGLRRATL
CCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
DDVADNEGDGSWAVAAEVEGALGLATLGTGIDPPRADAATSRRCRIDREPVGSATAFGDH
HHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
LRMVWLTPAMDGLFMGAASERRRFFDRLVLAIDSQHSGRVSALDRSLRSRNRLLEVRYPD
EEEEEECCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCC
AHWLDAIERETAELAVAVAAMRGQTAMRLAAMLDARGAASAFPSAKIMLDGWMESALLTE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCCCEEEEHHHHHHHHHCC
PATAVEDRYRTILREGRPRDAAAGRTLDGPHLTDLEVVYAPKAMPARDASTGEQKALLIG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
LVLAHAQLVSEMTGITPLLLLDEVVAHLDPSRRAALFEELAKLGAQVWMTGADPAAFAEI
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHC
GSGAEIFTVESGRIRPQQ
CCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TASRITRLTLTHFRNYRAAVLTTSAERVVLVGANGAGKTNCLEAISFLSPGRGLRRATL
CCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHH
DDVADNEGDGSWAVAAEVEGALGLATLGTGIDPPRADAATSRRCRIDREPVGSATAFGDH
HHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
LRMVWLTPAMDGLFMGAASERRRFFDRLVLAIDSQHSGRVSALDRSLRSRNRLLEVRYPD
EEEEEECCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCC
AHWLDAIERETAELAVAVAAMRGQTAMRLAAMLDARGAASAFPSAKIMLDGWMESALLTE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCCCEEEEHHHHHHHHHCC
PATAVEDRYRTILREGRPRDAAAGRTLDGPHLTDLEVVYAPKAMPARDASTGEQKALLIG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
LVLAHAQLVSEMTGITPLLLLDEVVAHLDPSRRAALFEELAKLGAQVWMTGADPAAFAEI
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHC
GSGAEIFTVESGRIRPQQ
CCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]