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The map label for this gene is purU [H]

Identifier: 162138522

GI number: 162138522

Start: 1777058

End: 1777921

Strand: Direct

Name: purU [H]

Synonym: RPB_1577

Alternate gene names: 162138522

Gene position: 1777058-1777921 (Clockwise)

Preceding gene: 86748690

Following gene: 86748704

Centisome position: 33.33

GC content: 64.81

Gene sequence:

>864_bases
ATGCCGCATCATCAATACGTGCTGACCCTGTCCTGCCCCGATCGCGTCGGCATCACGGCCGCGGTGACGACGTTCCTGTT
CGAGAACGGCCAGAACGTGCTCGACGCGCAACAATACAATGACACCGAGAGCGGTCATTTCTTCATGCGCGTGGTGTTCA
ACGCCGCCGACCGCGTGGTGCCGCTGGCGGAGCTGCGCACCGGCTTTTCCGCCATCGCCCTCAAATTCACGATGGGCTGG
CACATGCGCGACCGCAGCACCCGCCGGCGGGTGATGCTGCTGGTGTCGCAGTCCGATCACTGCCTCGCCGATATCCTGTA
TCGCTGGCGGATCGACGAGTTGCAGATGATCCCGACCGCGATCGTCTCCAACCATCCGCGCGACACCTTCAGCGGCTTCG
ATTTCGGCGAGATCCCGTTCTACCATCTGCCGGTGACCAAGGACACGCGGCGGCAGCAGGAGGCAGCGATCACCGCGCTG
ATCGCGCAGACCAAGACCGATCTCGTGGTGCTGGCGCGCTATATGCAGATCCTGTCGGACGAGATGGCGGGCCGCCTCGC
CGGGCGCTGCATCAACATCCACCATTCGTTCCTGCCCGGCTTCAAGGGCGCCAAGCCCTATCACCAGGCGTTCGACCGCG
GCGTCAAGCTGATCGGCGCCACCGCGCATTACGTCACCAGCACGCTCGACGAGGGCCCGATCATCGATCAGGACGTCGAG
CGCATCAGCCATCGCGACACCCCGGCCGATCTGGTGCGCAAGGGGCGCGACATCGAGCGCCGGGTGCTGGCCCGCGCGAT
GCACTATCACCTCGACGACCGGGTGATCCTCAACGGCCGCAAGACCGTGGTGTTCGTGGACTGA

Upstream 100 bases:

>100_bases
TGATCATCTTGCGCCTTTGATCGGGATTGGCGCAAGCCTCGCGCCGATGCTAGAGCAGGGGCCCGAACCGAGCGCGCCGC
CGCTGCTATCAAGATCGCCC

Downstream 100 bases:

>100_bases
TCGCGGCGGGTGGCGAGCGCTCAGCGCACCGCCGACGGCTCGGCGGGCTGTTGTCCGGTCTCGGCCGCCTTGGCGGCCTC
GATCTTCTGCCGCTCGGCCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPHHQYVLTLSCPDRVGITAAVTTFLFENGQNVLDAQQYNDTESGHFFMRVVFNAADRVVPLAELRTGFSAIALKFTMGW
HMRDRSTRRRVMLLVSQSDHCLADILYRWRIDELQMIPTAIVSNHPRDTFSGFDFGEIPFYHLPVTKDTRRQQEAAITAL
IAQTKTDLVVLARYMQILSDEMAGRLAGRCINIHHSFLPGFKGAKPYHQAFDRGVKLIGATAHYVTSTLDEGPIIDQDVE
RISHRDTPADLVRKGRDIERRVLARAMHYHLDDRVILNGRKTVVFVD

Sequences:

>Translated_287_residues
MPHHQYVLTLSCPDRVGITAAVTTFLFENGQNVLDAQQYNDTESGHFFMRVVFNAADRVVPLAELRTGFSAIALKFTMGW
HMRDRSTRRRVMLLVSQSDHCLADILYRWRIDELQMIPTAIVSNHPRDTFSGFDFGEIPFYHLPVTKDTRRQQEAAITAL
IAQTKTDLVVLARYMQILSDEMAGRLAGRCINIHHSFLPGFKGAKPYHQAFDRGVKLIGATAHYVTSTLDEGPIIDQDVE
RISHRDTPADLVRKGRDIERRVLARAMHYHLDDRVILNGRKTVVFVD
>Mature_286_residues
PHHQYVLTLSCPDRVGITAAVTTFLFENGQNVLDAQQYNDTESGHFFMRVVFNAADRVVPLAELRTGFSAIALKFTMGWH
MRDRSTRRRVMLLVSQSDHCLADILYRWRIDELQMIPTAIVSNHPRDTFSGFDFGEIPFYHLPVTKDTRRQQEAAITALI
AQTKTDLVVLARYMQILSDEMAGRLAGRCINIHHSFLPGFKGAKPYHQAFDRGVKLIGATAHYVTSTLDEGPIIDQDVER
ISHRDTPADLVRKGRDIERRVLARAMHYHLDDRVILNGRKTVVFVD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=119, Percent_Identity=33.6134453781513, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI209869995, Length=119, Percent_Identity=33.6134453781513, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI209869993, Length=119, Percent_Identity=33.6134453781513, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=38.2456140350877, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI1788846, Length=128, Percent_Identity=31.25, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=30.6010928961749, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32731; Mature: 32600

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHHQYVLTLSCPDRVGITAAVTTFLFENGQNVLDAQQYNDTESGHFFMRVVFNAADRVV
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCE
PLAELRTGFSAIALKFTMGWHMRDRSTRRRVMLLVSQSDHCLADILYRWRIDELQMIPTA
EHHHHHCCHHHEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHHHH
IVSNHPRDTFSGFDFGEIPFYHLPVTKDTRRQQEAAITALIAQTKTDLVVLARYMQILSD
HHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAGRLAGRCINIHHSFLPGFKGAKPYHQAFDRGVKLIGATAHYVTSTLDEGPIIDQDVE
HHHHHHHHHHEEEHHHHCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHH
RISHRDTPADLVRKGRDIERRVLARAMHYHLDDRVILNGRKTVVFVD
HHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEC
>Mature Secondary Structure 
PHHQYVLTLSCPDRVGITAAVTTFLFENGQNVLDAQQYNDTESGHFFMRVVFNAADRVV
CCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCE
PLAELRTGFSAIALKFTMGWHMRDRSTRRRVMLLVSQSDHCLADILYRWRIDELQMIPTA
EHHHHHCCHHHEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHHHH
IVSNHPRDTFSGFDFGEIPFYHLPVTKDTRRQQEAAITALIAQTKTDLVVLARYMQILSD
HHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAGRLAGRCINIHHSFLPGFKGAKPYHQAFDRGVKLIGATAHYVTSTLDEGPIIDQDVE
HHHHHHHHHHEEEHHHHCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCHHHHH
RISHRDTPADLVRKGRDIERRVLARAMHYHLDDRVILNGRKTVVFVD
HHHCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]