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The map label for this gene is algU [H]

Identifier: 162138520

GI number: 162138520

Start: 4062263

End: 4062832

Strand: Reverse

Name: algU [H]

Synonym: RPB_3549

Alternate gene names: 162138520

Gene position: 4062832-4062263 (Counterclockwise)

Preceding gene: 86750662

Following gene: 86750658

Centisome position: 76.2

GC content: 60.18

Gene sequence:

>570_bases
GTGAGTAACAAGCAGGCGACTGCGGACGAGGTGTTGATCGCCCGGATTGCTCAAGGCGACCGTTCTGCGATGCAGGTCCT
CTATGGCAGGCATCAGGTGCGGGTGTTTCGCTTCGGGCTGAGGCTGGTGCGGAACGAACAGATCGCGGAGGACCTTATCA
GTGAGGTCTTTCTTGATGTCTGGCGCCAGGCGGGGAAGTTCGAGGGGCGATCATCCGTTTCCACCTGGCTGTTGGCGATC
ACGCGGTTCAAGGCGCTCTCCGCGCTGCGACGCCGCAAGGATGCCGAACTGGACGACGAGGCTGCCGCCGCGATCGAGGA
TCCATCGGACGATCCGGAAGTGGCGGTCCAGAAAAAGGACACCAGCGATGTGTTGCGAAAGTGCCTCGAGGGGCTTTCGT
CGGACCATCGGGAGATCGTCGATCTCGTCTACTACCATGAGAAATCGGTGGAAGAGGTCGCTCATATTGTGGGAATTCCG
GAGAACACCGTGAAGACCCGACTGTTTTATGCGCGCAAGAAACTGGCCGATCTGCTGCAGGCAGCCGGCGTGCAGCGAGG
CTGGCCATGA

Upstream 100 bases:

>100_bases
ACCTCACAGAAGTGGCGTGGCACCTAGCTACGGTGATAAATTTGCCTTTGACTGTCGAACCTTCCGCGGGTTTCAGCCGA
CCTATTGTGCGAGACGGCCA

Downstream 100 bases:

>100_bases
TGGCGATGAGCAAATCGATGCTGGAGCATCAGGAGCCGGGCGACATCGAGGCGCTGCTGCCCTGGTATGCCGCGGGCACG
CTGAATGCCCGCGACAGCGA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSNKQATADEVLIARIAQGDRSAMQVLYGRHQVRVFRFGLRLVRNEQIAEDLISEVFLDVWRQAGKFEGRSSVSTWLLAI
TRFKALSALRRRKDAELDDEAAAAIEDPSDDPEVAVQKKDTSDVLRKCLEGLSSDHREIVDLVYYHEKSVEEVAHIVGIP
ENTVKTRLFYARKKLADLLQAAGVQRGWP

Sequences:

>Translated_189_residues
MSNKQATADEVLIARIAQGDRSAMQVLYGRHQVRVFRFGLRLVRNEQIAEDLISEVFLDVWRQAGKFEGRSSVSTWLLAI
TRFKALSALRRRKDAELDDEAAAAIEDPSDDPEVAVQKKDTSDVLRKCLEGLSSDHREIVDLVYYHEKSVEEVAHIVGIP
ENTVKTRLFYARKKLADLLQAAGVQRGWP
>Mature_188_residues
SNKQATADEVLIARIAQGDRSAMQVLYGRHQVRVFRFGLRLVRNEQIAEDLISEVFLDVWRQAGKFEGRSSVSTWLLAIT
RFKALSALRRRKDAELDDEAAAAIEDPSDDPEVAVQKKDTSDVLRKCLEGLSSDHREIVDLVYYHEKSVEEVAHIVGIPE
NTVKTRLFYARKKLADLLQAAGVQRGWP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=183, Percent_Identity=28.4153005464481, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21406; Mature: 21274

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKQATADEVLIARIAQGDRSAMQVLYGRHQVRVFRFGLRLVRNEQIAEDLISEVFLDV
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRQAGKFEGRSSVSTWLLAITRFKALSALRRRKDAELDDEAAAAIEDPSDDPEVAVQKKD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEECCC
TSDVLRKCLEGLSSDHREIVDLVYYHEKSVEEVAHIVGIPENTVKTRLFYARKKLADLLQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAGVQRGWP
HCCCCCCCC
>Mature Secondary Structure 
SNKQATADEVLIARIAQGDRSAMQVLYGRHQVRVFRFGLRLVRNEQIAEDLISEVFLDV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRQAGKFEGRSSVSTWLLAITRFKALSALRRRKDAELDDEAAAAIEDPSDDPEVAVQKKD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEECCC
TSDVLRKCLEGLSSDHREIVDLVYYHEKSVEEVAHIVGIPENTVKTRLFYARKKLADLLQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AAGVQRGWP
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]