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The map label for this gene is phoB [C]

Identifier: 86610276

GI number: 86610276

Start: 2952637

End: 2953380

Strand: Direct

Name: phoB [C]

Synonym: CYB_2856

Alternate gene names: 86610276

Gene position: 2952637-2953380 (Clockwise)

Preceding gene: 86610271

Following gene: 86610280

Centisome position: 96.91

GC content: 57.8

Gene sequence:

>744_bases
ATGGAAGCAACCCTCTCCATGACTGGTTCACTTCCTCAAGCCAAGGAAGCTGCTCCCCATCTGGAGACCCTGCTGGTGGT
GGATGACGAGGATGCCATTCGGGAAACGGTTGCCCTGGCTTTAGAAGAGCAGGGGTATCAGGTTTTACAAGCCGCTGATG
GTCGGCAAGCCCTTGAGATGGTGCATACGGTTGAGCGGCTGGATTTGATGATCTTGGATTTGATGTTGCCCAGCTTGAAC
GGTCTGGATCTGTGCAGGCTGCTGCGCAGGGAGGGCAACACGATTCCCATCTTGATGTTGACGGCCAAAAGTAGCGAAAC
CGACCGCGTGGTGGGCTTGGAACTGGGGGCCGACGATTATCTGACCAAGCCCTTCGGCATGCGGGAGCTGATCGCCCGCT
GTCGCTCGGTGTTGCGCCGGCAACAACTGGCCCAAGCCAGCGATGCCACGGTGCTCCGTTACGGGGATCTGTGTATGTTT
ACCCAGGAGTGCCGCGTGTTGGTGCGGGGCGTTGAGGTGAGTTTTTCCCCCAAGGAATTTCGCCTGTTGGAGCTGTTTAT
GTCCAATCCCCGCCGCGTTTGGACGCGGGAACAGCTTCTGGAGCGCATCTGGGGATCCGACTTCATGGGCGACAGCAAGA
CCGTCGATGTACATATCCGCTGGCTGCGGGAAAAATTGGAGCTGGATCCCAGTAACCCAGAGTATTTGGTGACGGTGCGC
GGCTTCGGCTATCGCTTCGGCTAG

Upstream 100 bases:

>100_bases
CTTGCCCTTCTCACCCTGTGAGGCTGTTATCGCTACGTGACGCTTCCGCGTTCGTGACGGCGGTGCGGAGCACTTGCGTG
CAGGCGCAACGGAGTTCTGT

Downstream 100 bases:

>100_bases
TCTTTTTGCTGGCCACGCCACCGGCGGGCAAAAGAGAGCGCCTCGGCACGAGTTTGAATCTGCCCCCAAGCCTGCGCCTC
CTGGAGGTCTTGCAGCAACT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MEATLSMTGSLPQAKEAAPHLETLLVVDDEDAIRETVALALEEQGYQVLQAADGRQALEMVHTVERLDLMILDLMLPSLN
GLDLCRLLRREGNTIPILMLTAKSSETDRVVGLELGADDYLTKPFGMRELIARCRSVLRRQQLAQASDATVLRYGDLCMF
TQECRVLVRGVEVSFSPKEFRLLELFMSNPRRVWTREQLLERIWGSDFMGDSKTVDVHIRWLREKLELDPSNPEYLVTVR
GFGYRFG

Sequences:

>Translated_247_residues
MEATLSMTGSLPQAKEAAPHLETLLVVDDEDAIRETVALALEEQGYQVLQAADGRQALEMVHTVERLDLMILDLMLPSLN
GLDLCRLLRREGNTIPILMLTAKSSETDRVVGLELGADDYLTKPFGMRELIARCRSVLRRQQLAQASDATVLRYGDLCMF
TQECRVLVRGVEVSFSPKEFRLLELFMSNPRRVWTREQLLERIWGSDFMGDSKTVDVHIRWLREKLELDPSNPEYLVTVR
GFGYRFG
>Mature_247_residues
MEATLSMTGSLPQAKEAAPHLETLLVVDDEDAIRETVALALEEQGYQVLQAADGRQALEMVHTVERLDLMILDLMLPSLN
GLDLCRLLRREGNTIPILMLTAKSSETDRVVGLELGADDYLTKPFGMRELIARCRSVLRRQQLAQASDATVLRYGDLCMF
TQECRVLVRGVEVSFSPKEFRLLELFMSNPRRVWTREQLLERIWGSDFMGDSKTVDVHIRWLREKLELDPSNPEYLVTVR
GFGYRFG

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=43.8053097345133, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=40.0881057268723, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=39.647577092511, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=38.9140271493213, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=36.1702127659575, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=36.322869955157, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=38.4955752212389, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789402, Length=224, Percent_Identity=37.0535714285714, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.7713004484305, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=36.1607142857143, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=36.2445414847162, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787229, Length=231, Percent_Identity=36.3636363636364, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=31.6964285714286, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=29.6610169491525, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=28.8, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788713, Length=109, Percent_Identity=34.8623853211009, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787487, Length=117, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28147; Mature: 28147

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATLSMTGSLPQAKEAAPHLETLLVVDDEDAIRETVALALEEQGYQVLQAADGRQALEM
CCCEECCCCCCCCHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
VHTVERLDLMILDLMLPSLNGLDLCRLLRREGNTIPILMLTAKSSETDRVVGLELGADDY
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEECCCCC
LTKPFGMRELIARCRSVLRRQQLAQASDATVLRYGDLCMFTQECRVLVRGVEVSFSPKEF
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCEECCCHHH
RLLELFMSNPRRVWTREQLLERIWGSDFMGDSKTVDVHIRWLREKLELDPSNPEYLVTVR
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEEEEE
GFGYRFG
CCCCCCC
>Mature Secondary Structure
MEATLSMTGSLPQAKEAAPHLETLLVVDDEDAIRETVALALEEQGYQVLQAADGRQALEM
CCCEECCCCCCCCHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHH
VHTVERLDLMILDLMLPSLNGLDLCRLLRREGNTIPILMLTAKSSETDRVVGLELGADDY
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEECCCCC
LTKPFGMRELIARCRSVLRRQQLAQASDATVLRYGDLCMFTQECRVLVRGVEVSFSPKEF
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCEECCCHHH
RLLELFMSNPRRVWTREQLLERIWGSDFMGDSKTVDVHIRWLREKLELDPSNPEYLVTVR
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEEEEE
GFGYRFG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]