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The map label for this gene is phoB [H]

Identifier: 86609986

GI number: 86609986

Start: 2659064

End: 2659702

Strand: Direct

Name: phoB [H]

Synonym: CYB_2553

Alternate gene names: 86609986

Gene position: 2659064-2659702 (Clockwise)

Preceding gene: 86609983

Following gene: 86609987

Centisome position: 87.28

GC content: 55.24

Gene sequence:

>639_bases
GTGGACAACCCGAAGCGACTGTTGCTCATCGATGACGATCCGAACTTAATCCTGCTGGCCAAGGACTACCTAGAGTTTCG
CGGCTACGAAGTGGTGGCGGCAAGCAACGGTCTGGAGGGGTTGGAAGCGATGCGCCATTTCACTCCAGACCTGATCATCT
GCGATGTGATGATGCCGGAGATGGATGGCTATACCTTTGTGGAAACCATTCGCTCCAACAGTGCTACAGACTGGATACCT
GTGATTTTTCTGTCGGCGCGTGGGCAGACGGCGGATCGGGTGCGCGGCCTGACCACAGGGGCAGATGTTTACATGGTCAA
GCCCTTTGAGCCGGAAGAATTGGTGGCGCAGGTGGAATCTTCTCTCAAACATACCGAGCGCCTGTTACAAGTGCAGAATG
TGGGCATTCAGCCGGTCATAAAACTGGATCGGGAAATTGAGCTGACGCCAACAGAAACCAAGGTGATCCAGTACGTGGCC
AGGGGCCTGTCCAACCGCGAGATTGCCGAAGTTCTTGGGGTGAGCCAGCGCACCGTTGAAAGCCACGTCAGCAACATGCT
GGGCAAAACCGGCCTGCACAACCGCACAGAACTGGCCCGCTGGGCAATAGAAAGTGGTTTGGGCAAAAAACCGTTGTAA

Upstream 100 bases:

>100_bases
GGATTGTTTTCCTGCGGTGAAGATCCTCAAAGCAGGAGGGGTTGTGTTTTTTCCCTAGAGCTTCTTAGCACTCAACTTTT
TAGCACTCAAAAGGTGAGCT

Downstream 100 bases:

>100_bases
TTCCCGCCACCCCTTTCAACGGCTGGAGAACTCTATCTCCTCGGCGCGATTGGAGATAGGGTAGTGATCGCACTTGAAGG
GCAAGGTAACTGGGGTGGGC

Product: LuxR family DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDNPKRLLLIDDDPNLILLAKDYLEFRGYEVVAASNGLEGLEAMRHFTPDLIICDVMMPEMDGYTFVETIRSNSATDWIP
VIFLSARGQTADRVRGLTTGADVYMVKPFEPEELVAQVESSLKHTERLLQVQNVGIQPVIKLDREIELTPTETKVIQYVA
RGLSNREIAEVLGVSQRTVESHVSNMLGKTGLHNRTELARWAIESGLGKKPL

Sequences:

>Translated_212_residues
MDNPKRLLLIDDDPNLILLAKDYLEFRGYEVVAASNGLEGLEAMRHFTPDLIICDVMMPEMDGYTFVETIRSNSATDWIP
VIFLSARGQTADRVRGLTTGADVYMVKPFEPEELVAQVESSLKHTERLLQVQNVGIQPVIKLDREIELTPTETKVIQYVA
RGLSNREIAEVLGVSQRTVESHVSNMLGKTGLHNRTELARWAIESGLGKKPL
>Mature_212_residues
MDNPKRLLLIDDDPNLILLAKDYLEFRGYEVVAASNGLEGLEAMRHFTPDLIICDVMMPEMDGYTFVETIRSNSATDWIP
VIFLSARGQTADRVRGLTTGADVYMVKPFEPEELVAQVESSLKHTERLLQVQNVGIQPVIKLDREIELTPTETKVIQYVA
RGLSNREIAEVLGVSQRTVESHVSNMLGKTGLHNRTELARWAIESGLGKKPL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=211, Percent_Identity=30.8056872037915, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788394, Length=207, Percent_Identity=31.8840579710145, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI2367329, Length=206, Percent_Identity=34.4660194174757, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082012, Length=123, Percent_Identity=39.8373983739837, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=33.0645161290323, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790437, Length=181, Percent_Identity=32.0441988950276, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786784, Length=165, Percent_Identity=32.1212121212121, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1788521, Length=199, Percent_Identity=28.643216080402, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786747, Length=167, Percent_Identity=29.940119760479, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=32.2033898305085, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789402, Length=203, Percent_Identity=31.0344827586207, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786911, Length=160, Percent_Identity=31.25, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790860, Length=105, Percent_Identity=34.2857142857143, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787473, Length=206, Percent_Identity=33.0097087378641, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=28.9340101522843, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=32.5396825396825, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788905, Length=122, Percent_Identity=35.2459016393443, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787487, Length=106, Percent_Identity=33.9622641509434, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790552, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787229, Length=129, Percent_Identity=29.4573643410853, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1790863, Length=122, Percent_Identity=28.6885245901639, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788713, Length=138, Percent_Identity=32.6086956521739, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 23748; Mature: 23748

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNPKRLLLIDDDPNLILLAKDYLEFRGYEVVAASNGLEGLEAMRHFTPDLIICDVMMPE
CCCCCEEEEEECCCCEEEEEHHHHHHCCEEEEEECCCHHHHHHHHHCCCCEEEEEEECCC
MDGYTFVETIRSNSATDWIPVIFLSARGQTADRVRGLTTGADVYMVKPFEPEELVAQVES
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEECCCCHHHHHHHHHH
SLKHTERLLQVQNVGIQPVIKLDREIELTPTETKVIQYVARGLSNREIAEVLGVSQRTVE
HHHHHHHHHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHH
SHVSNMLGKTGLHNRTELARWAIESGLGKKPL
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDNPKRLLLIDDDPNLILLAKDYLEFRGYEVVAASNGLEGLEAMRHFTPDLIICDVMMPE
CCCCCEEEEEECCCCEEEEEHHHHHHCCEEEEEECCCHHHHHHHHHCCCCEEEEEEECCC
MDGYTFVETIRSNSATDWIPVIFLSARGQTADRVRGLTTGADVYMVKPFEPEELVAQVES
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHCCCCCCCEEEECCCCHHHHHHHHHH
SLKHTERLLQVQNVGIQPVIKLDREIELTPTETKVIQYVARGLSNREIAEVLGVSQRTVE
HHHHHHHHHHHHCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHH
SHVSNMLGKTGLHNRTELARWAIESGLGKKPL
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]