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The map label for this gene is thiG [H]

Identifier: 86609833

GI number: 86609833

Start: 2495573

End: 2496349

Strand: Direct

Name: thiG [H]

Synonym: CYB_2395

Alternate gene names: 86609833

Gene position: 2495573-2496349 (Clockwise)

Preceding gene: 86609832

Following gene: 86609835

Centisome position: 81.91

GC content: 65.25

Gene sequence:

>777_bases
TTGGTTCTCTACGGCCAACCGTTACCCAGCCGTCTGATGTTGGGTACGGCTCGCTACCCTTCGCCGGCCATTTTGGAGCA
GGCGGTGGCGCGCGCGCAGCCCTGTTTGTTGACGGTTTCTCTGCGGCGGCAAGGGGCACTGGGGCCAGAAACTTCCCAGA
GGTTCTGGGCTCTGTTGCAGAAGATGGGGATCCCAGTTCTGCCCAACACCGCCGGCTGCCACAGCGTGCAGGAGGTGATC
ACAACGGCGGAGATGGCGCGGGAGGTGTTCGGCACCGACTGGATCAAGCTGGAGTTGATTGGGGATGACTACACGCTGCA
GCCGGATCCCGTGCAACTGCCCACTGCTGCTGCTGAGCTGGTCCGGCGCGGCTTTAAGGTGCTGCCCTATAGCACCGACG
ACCTGGTGCTCTGCCAGCGGCTTTTGGATGCTGGCTGTGAGGTGGTGATGCCCTGGGCGGCCCCCATCGGCACCGGCAAA
GGCCCTCTCAACCCCTACAACTTGCGCCTGCTGCGGGAGCGGATCGAGGCGCCGCTGATCGTGGATGCCGGCTTGGGCCT
GCCCTCCCATGCCTGCCAGGTGATGGAGTGGGGCTTCGACGGGGTGCTGCTAAACACGGCGGTGGCCTTGGCCCGGGATC
CGGTGGCCATGGCCGAGGCCTTTGCAGAAGCGGTGCGCTCAGGCCGCCGAGCCTACCTGGCCGGGGCCATGATCCCTCAG
GAAACAGCTCAGCCCAGTACGCCTGTCCTCGGCACTCCCTTTTGGCACCAGGGCTGA

Upstream 100 bases:

>100_bases
CGGTTGCCGGCGGCTAGCGCTCAAGTCCGCTTTTCTATCTGCCTCAGCCATGTCCACCCTTCTCCATTGCTCTCCCTCAA
CTCTGGCCGATGAGGATCCT

Downstream 100 bases:

>100_bases
CTGCCGCTCTCAGGGGTGCAGCTCAGGCGGCAGGCTTTCGCGCCTGGATAACCTGCAGAGCTCCCCCAGCGTGGAGGGTC
TGCCGGATGGATTGCCACCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MVLYGQPLPSRLMLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPETSQRFWALLQKMGIPVLPNTAGCHSVQEVI
TTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPTAAAELVRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGK
GPLNPYNLRLLRERIEAPLIVDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMIPQ
ETAQPSTPVLGTPFWHQG

Sequences:

>Translated_258_residues
MVLYGQPLPSRLMLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPETSQRFWALLQKMGIPVLPNTAGCHSVQEVI
TTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPTAAAELVRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGK
GPLNPYNLRLLRERIEAPLIVDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMIPQ
ETAQPSTPVLGTPFWHQG
>Mature_258_residues
MVLYGQPLPSRLMLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPETSQRFWALLQKMGIPVLPNTAGCHSVQEVI
TTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPTAAAELVRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGK
GPLNPYNLRLLRERIEAPLIVDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMIPQ
ETAQPSTPVLGTPFWHQG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=249, Percent_Identity=39.3574297188755, Blast_Score=176, Evalue=9e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 27949; Mature: 27949

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLYGQPLPSRLMLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPETSQRFWALLQ
CEEECCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEHHCCCCCCCHHHHHHHHHH
KMGIPVLPNTAGCHSVQEVITTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPTAAAEL
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
VRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKGPLNPYNLRLLRERIEAPLI
HHCCCEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMIPQ
EECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHHCCCCC
ETAQPSTPVLGTPFWHQG
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVLYGQPLPSRLMLGTARYPSPAILEQAVARAQPCLLTVSLRRQGALGPETSQRFWALLQ
CEEECCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEHHCCCCCCCHHHHHHHHHH
KMGIPVLPNTAGCHSVQEVITTAEMAREVFGTDWIKLELIGDDYTLQPDPVQLPTAAAEL
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
VRRGFKVLPYSTDDLVLCQRLLDAGCEVVMPWAAPIGTGKGPLNPYNLRLLRERIEAPLI
HHCCCEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
VDAGLGLPSHACQVMEWGFDGVLLNTAVALARDPVAMAEAFAEAVRSGRRAYLAGAMIPQ
EECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEHHHCCCCC
ETAQPSTPVLGTPFWHQG
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA