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The map label for this gene is ndhJ

Identifier: 86609774

GI number: 86609774

Start: 2439910

End: 2440461

Strand: Direct

Name: ndhJ

Synonym: CYB_2334

Alternate gene names: 86609774

Gene position: 2439910-2440461 (Clockwise)

Preceding gene: 86609773

Following gene: 86609775

Centisome position: 80.08

GC content: 58.15

Gene sequence:

>552_bases
ATGGCAGAGGAAAATGCCCAGGAAAAACAAGCGCCGCCATCCGCAGGCGAGCAGTCGGAACCCTTGGTGACGTTGGAGCG
GGGCCCGGTTTCCCAGTTTCTGGCCGACAACGGCTTCGAGCACCAGTATTTGGGGAGAGACGCAGCGGGGGTAGAGCTGT
TGGAGGTGGAGCGGGACTTTCTCCTACCCTTGTGTACCGCCCTCTATGCCTACGGCTTTAACTACCTGGAGTGTCAGTGC
GGATATGACCTGGGGGCAGGGCAGCCACTGGTCAGCCTTTACCACCTGATCAAGCTGTCAGACGGGGCGGATCGCCCTCA
GGAGGTGCGGCTCCAGGTGAAGTTGCCTCGCCAGGATCCACGCCTACCTTCCGTATACTGGATTTGGAAGTCGGCGGATT
GGCAGGAACGGGAAACCTACGACATGTACGGCATTGTTTTCGAGGGACACCCGAACCTGAAGCGGATTTTAATGCCGGAA
GACTGGATAGGTTGGCCCCTGCGCAAGGACTACATCACGCCTGATTTCTATGAGCTGCAGGATGCCTACTGA

Upstream 100 bases:

>100_bases
CTGGCTGCGGCCATTGGCTTGCCAGTGCCCCCGGCTTTGCAAACTGCCGACTTTAAGCAGGCGGAACAGCAGCTCAAAGC
CTTGCAGGGAGGAGTGTGAG

Downstream 100 bases:

>100_bases
TTGGGGATCCCTGACCTGGTGAGCAGCGTTGAACAGGATGGGTTGAGATGTGGCCTTTTTTGCTCTTGGCAATCTACGCA
GGAGGAGTTTGGTACTCGGC

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MAEENAQEKQAPPSAGEQSEPLVTLERGPVSQFLADNGFEHQYLGRDAAGVELLEVERDFLLPLCTALYAYGFNYLECQC
GYDLGAGQPLVSLYHLIKLSDGADRPQEVRLQVKLPRQDPRLPSVYWIWKSADWQERETYDMYGIVFEGHPNLKRILMPE
DWIGWPLRKDYITPDFYELQDAY

Sequences:

>Translated_183_residues
MAEENAQEKQAPPSAGEQSEPLVTLERGPVSQFLADNGFEHQYLGRDAAGVELLEVERDFLLPLCTALYAYGFNYLECQC
GYDLGAGQPLVSLYHLIKLSDGADRPQEVRLQVKLPRQDPRLPSVYWIWKSADWQERETYDMYGIVFEGHPNLKRILMPE
DWIGWPLRKDYITPDFYELQDAY
>Mature_182_residues
AEENAQEKQAPPSAGEQSEPLVTLERGPVSQFLADNGFEHQYLGRDAAGVELLEVERDFLLPLCTALYAYGFNYLECQCG
YDLGAGQPLVSLYHLIKLSDGADRPQEVRLQVKLPRQDPRLPSVYWIWKSADWQERETYDMYGIVFEGHPNLKRILMPED
WIGWPLRKDYITPDFYELQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family

Homologues:

Organism=Escherichia coli, GI145693162, Length=79, Percent_Identity=48.1012658227848, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHJ_SYNJB (Q2JJA8)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478536.1
- ProteinModelPortal:   Q2JJA8
- SMR:   Q2JJA8
- STRING:   Q2JJA8
- GeneID:   3901092
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2334
- TIGR:   CYB_2334
- eggNOG:   COG0852
- HOGENOM:   HBG727675
- OMA:   YELQDAY
- ProtClustDB:   PRK12494
- BioCyc:   SSP321332:CYB_2334-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01357
- InterPro:   IPR010218
- InterPro:   IPR001268
- InterPro:   IPR020396
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa

EC number: 1.6.5.3

Molecular weight: Translated: 21134; Mature: 21003

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEENAQEKQAPPSAGEQSEPLVTLERGPVSQFLADNGFEHQYLGRDAAGVELLEVERDF
CCCCCCCHHCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCHHCCCCCCCEEEEEHHHHH
LLPLCTALYAYGFNYLECQCGYDLGAGQPLVSLYHLIKLSDGADRPQEVRLQVKLPRQDP
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
RLPSVYWIWKSADWQERETYDMYGIVFEGHPNLKRILMPEDWIGWPLRKDYITPDFYELQ
CCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHC
DAY
CCC
>Mature Secondary Structure 
AEENAQEKQAPPSAGEQSEPLVTLERGPVSQFLADNGFEHQYLGRDAAGVELLEVERDF
CCCCCCHHCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCHHCCCCCCCEEEEEHHHHH
LLPLCTALYAYGFNYLECQCGYDLGAGQPLVSLYHLIKLSDGADRPQEVRLQVKLPRQDP
HHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
RLPSVYWIWKSADWQERETYDMYGIVFEGHPNLKRILMPEDWIGWPLRKDYITPDFYELQ
CCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHC
DAY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA