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The map label for this gene is ndhK [H]

Identifier: 86609773

GI number: 86609773

Start: 2439174

End: 2439908

Strand: Direct

Name: ndhK [H]

Synonym: CYB_2333

Alternate gene names: 86609773

Gene position: 2439174-2439908 (Clockwise)

Preceding gene: 86609772

Following gene: 86609774

Centisome position: 80.06

GC content: 59.86

Gene sequence:

>735_bases
ATGACGTCTGAGAAATCGCAAGTCTTGTTTCCGGGGGCTGCCCCCCAGGTGACTACCGACCTCTCCAACAACGTCGTTCT
GACGACGGTGAACGACCTGTACAACTGGGCCAAGATGTCCAGCCTCTGGCCGCTGCTCTACGGCACTGCCTGTTGCTTCA
TCGAGTTTGCTGCCATGCTGGGATCCCGCTTCGACTTCGACCGCTTTGGCCTGTTGCCTCGTTCTTCGCCACGCACCGCT
GATTTGATCATCACGGCGGGCACGGTCACCATGAAGATGGCTCCGGCGTTGGTCAAGCTTTACCAGCAAATGGCTGAGCC
GAAGTACGTGATTGCCATGGGGGCCTGCACCATCAGCGGTGGCATGTTCAGCTCCGATTCCTACACGGCAGTGCGGGGGG
TGGACAAGCTGATCCCGGTGGATGTGTACATTCCCGGCTGCCCCCCTCGTCCAGAGGCGATCATGGATGCCATTGTCAAG
CTGCGCAAGAAGATCGCCGCCGAGGACATGCGGGAACGAGGCCGTCTGCAGCAAACCCACCGCTACTACACGGTTAAGCA
CAACCTCAAGCCTGTGCCGGAGATCATCACCGGCAAGTATCTGGAGAGCGAGACCCGCCAAGCTCCCCCGCCAGAACTGG
CTGCGGCCATTGGCTTGCCAGTGCCCCCGGCTTTGCAAACTGCCGACTTTAAGCAGGCGGAACAGCAGCTCAAAGCCTTG
CAGGGAGGAGTGTGA

Upstream 100 bases:

>100_bases
ACCGCTTGGGGCTGTTGGCCTTTGTGGAGGCCCTGATTTTCATCGCCATCCTTGTGGTGGGTCTTGTGTACGCCTGGAGA
AAAGGAGCATTGGAATGGTC

Downstream 100 bases:

>100_bases
GATGGCAGAGGAAAATGCCCAGGAAAAACAAGCGCCGCCATCCGCAGGCGAGCAGTCGGAACCCTTGGTGACGTTGGAGC
GGGGCCCGGTTTCCCAGTTT

Product: NADH dehydrogenase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTSEKSQVLFPGAAPQVTTDLSNNVVLTTVNDLYNWAKMSSLWPLLYGTACCFIEFAAMLGSRFDFDRFGLLPRSSPRTA
DLIITAGTVTMKMAPALVKLYQQMAEPKYVIAMGACTISGGMFSSDSYTAVRGVDKLIPVDVYIPGCPPRPEAIMDAIVK
LRKKIAAEDMRERGRLQQTHRYYTVKHNLKPVPEIITGKYLESETRQAPPPELAAAIGLPVPPALQTADFKQAEQQLKAL
QGGV

Sequences:

>Translated_244_residues
MTSEKSQVLFPGAAPQVTTDLSNNVVLTTVNDLYNWAKMSSLWPLLYGTACCFIEFAAMLGSRFDFDRFGLLPRSSPRTA
DLIITAGTVTMKMAPALVKLYQQMAEPKYVIAMGACTISGGMFSSDSYTAVRGVDKLIPVDVYIPGCPPRPEAIMDAIVK
LRKKIAAEDMRERGRLQQTHRYYTVKHNLKPVPEIITGKYLESETRQAPPPELAAAIGLPVPPALQTADFKQAEQQLKAL
QGGV
>Mature_243_residues
TSEKSQVLFPGAAPQVTTDLSNNVVLTTVNDLYNWAKMSSLWPLLYGTACCFIEFAAMLGSRFDFDRFGLLPRSSPRTAD
LIITAGTVTMKMAPALVKLYQQMAEPKYVIAMGACTISGGMFSSDSYTAVRGVDKLIPVDVYIPGCPPRPEAIMDAIVKL
RKKIAAEDMRERGRLQQTHRYYTVKHNLKPVPEIITGKYLESETRQAPPPELAAAIGLPVPPALQTADFKQAEQQLKALQ
GGV

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=156, Percent_Identity=51.9230769230769, Blast_Score=174, Evalue=5e-44,
Organism=Escherichia coli, GI1788624, Length=165, Percent_Identity=47.8787878787879, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789074, Length=108, Percent_Identity=45.3703703703704, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788834, Length=129, Percent_Identity=38.7596899224806, Blast_Score=94, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=139, Percent_Identity=49.6402877697842, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI18859983, Length=139, Percent_Identity=51.7985611510791, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24642371, Length=139, Percent_Identity=51.7985611510791, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24651058, Length=136, Percent_Identity=51.4705882352941, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 26754; Mature: 26623

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEKSQVLFPGAAPQVTTDLSNNVVLTTVNDLYNWAKMSSLWPLLYGTACCFIEFAAML
CCCCCCCEEECCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSRFDFDRFGLLPRSSPRTADLIITAGTVTMKMAPALVKLYQQMAEPKYVIAMGACTISG
CCCCCHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECC
GMFSSDSYTAVRGVDKLIPVDVYIPGCPPRPEAIMDAIVKLRKKIAAEDMRERGRLQQTH
CCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYYTVKHNLKPVPEIITGKYLESETRQAPPPELAAAIGLPVPPALQTADFKQAEQQLKAL
HEEEEECCCCCCHHHHHCHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QGGV
HCCC
>Mature Secondary Structure 
TSEKSQVLFPGAAPQVTTDLSNNVVLTTVNDLYNWAKMSSLWPLLYGTACCFIEFAAML
CCCCCCEEECCCCCCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSRFDFDRFGLLPRSSPRTADLIITAGTVTMKMAPALVKLYQQMAEPKYVIAMGACTISG
CCCCCHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECEEECC
GMFSSDSYTAVRGVDKLIPVDVYIPGCPPRPEAIMDAIVKLRKKIAAEDMRERGRLQQTH
CCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYYTVKHNLKPVPEIITGKYLESETRQAPPPELAAAIGLPVPPALQTADFKQAEQQLKAL
HEEEEECCCCCCHHHHHCHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
QGGV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA