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The map label for this gene is rpoD [H]

Identifier: 86609652

GI number: 86609652

Start: 2311744

End: 2312955

Strand: Reverse

Name: rpoD [H]

Synonym: CYB_2206

Alternate gene names: 86609652

Gene position: 2312955-2311744 (Counterclockwise)

Preceding gene: 86609655

Following gene: 86609651

Centisome position: 75.92

GC content: 56.19

Gene sequence:

>1212_bases
ATGGCTCAAGCAAAGGAATTGGTGAAATCGGGTAAAACAGCTCTTCTCGAATCGGATCTGGAAAATCCACCTTCTGGCAC
CCTGGAGTTGGACACGGAGACAGATACCCTCGAGAGCGAACTGGATACCGGTGACACTGATGCTTTTGATGAGGATGACG
ACGACGAAGAGGCAGCAGAGGAGGATGGAGAGACTGCCGCCGCCGGTGCCAAAGGTCGCTCAAAGCGCAAGGCGGCCAGT
AAGAAAAAGCCCTACACCGATGACTCTATCCGGGTCTATTTGCAAGAAATTGGGCGTATCCGGCTGCTGCGGGCCGATGA
AGAAATCGAGCTAGCCCGAAAAATTGCCGATCTGCTGGAGCTAGAGCGCGCTCGCGAGGCCGTTTTTCAGCGTATGGGCC
TCTGGTCAGACCCAGAAGAGGTGCCGGACACCCTCTGGGCAGAAGAGGTCAAGATGTCCTTGCCGGAATTTCGGCGGCGG
TTGCATCGGGGGCGGCGGGCCAAAGAAAAGATGGTGCAGTCTAATCTGCGTTTGGTGGTGTCCATCGCCAAAAAGTACAT
GAACCGGGGCCTGTCTTTTCAGGATTTGATTCAGGAGGGATCCCTGGGCCTGATTCGGGCAGCCGAGAAATTCGACCACG
AGAAGGGCTACAAGTTCTCCACCTATGCGACGTGGTGGATTCGACAGGCCATCACCCGCGCCATTGCCGACCAATCCCGC
ACCATTCGCCTGCCTGTCCACCTCTACGAGACGATCTCCCGCATCAAAAAGGTAACCAAGTTGCTTTCCCAGGAATTGGG
CCGCAAGCCCACCGAAGAGGAGATCGCCACCCGCATGGAGATCACCATCGAGAAGTTGCGCTTTATCGCCAAGTCGGCCC
AGCTTCCCATCTCTTTGGAAACCCCCATCGGCAAGGAAGAGGATTCTCGGCTGGGAGACTTTATCGAGTCGGATGGGGAG
ACGCCGGAGGATCGGGTGGCCAAAGTCTTGCTCAGAGAAGATCTGGAAAGCGTTTTGGAAACCCTCACCGCCCGCGAACG
GGATGTGTTGAAGTTGCGCTACGGCTTGGATGATGGCCGCATGAAAACTCTGGAGGAAATCGGCCAGATCTTTAATGTCA
CCCGTGAGCGAATCCGGCAGATCGAGGCTAAGGCTCTGCGCAAGTTGCGCCACCCCAACCGCAACAGCGTGTTGAAGGAA
TACATCCGGTGA

Upstream 100 bases:

>100_bases
GTGGGATGCAGCAGGGCTGCAACTTGCTCCTCTCCCTTTGGCGTCTAAGCTCCCTTAGTCACCTTTAGTTGTCTGTGATT
AGGATCACGAGGAACTCAGC

Downstream 100 bases:

>100_bases
CTCTGGGGCCCATTCCTTCTAGCTCTCAGGGAGAGCCGGGGCAAGGGATCCAAGAGAGTTCCCGGCCCCCTAAGGCAGAC
TGAGTTGGAACTGCCAACCC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAQAKELVKSGKTALLESDLENPPSGTLELDTETDTLESELDTGDTDAFDEDDDDEEAAEEDGETAAAGAKGRSKRKAAS
KKKPYTDDSIRVYLQEIGRIRLLRADEEIELARKIADLLELERAREAVFQRMGLWSDPEEVPDTLWAEEVKMSLPEFRRR
LHRGRRAKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSR
TIRLPVHLYETISRIKKVTKLLSQELGRKPTEEEIATRMEITIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGE
TPEDRVAKVLLREDLESVLETLTARERDVLKLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKE
YIR

Sequences:

>Translated_403_residues
MAQAKELVKSGKTALLESDLENPPSGTLELDTETDTLESELDTGDTDAFDEDDDDEEAAEEDGETAAAGAKGRSKRKAAS
KKKPYTDDSIRVYLQEIGRIRLLRADEEIELARKIADLLELERAREAVFQRMGLWSDPEEVPDTLWAEEVKMSLPEFRRR
LHRGRRAKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSR
TIRLPVHLYETISRIKKVTKLLSQELGRKPTEEEIATRMEITIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGE
TPEDRVAKVLLREDLESVLETLTARERDVLKLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKE
YIR
>Mature_402_residues
AQAKELVKSGKTALLESDLENPPSGTLELDTETDTLESELDTGDTDAFDEDDDDEEAAEEDGETAAAGAKGRSKRKAASK
KKPYTDDSIRVYLQEIGRIRLLRADEEIELARKIADLLELERAREAVFQRMGLWSDPEEVPDTLWAEEVKMSLPEFRRRL
HRGRRAKEKMVQSNLRLVVSIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRT
IRLPVHLYETISRIKKVTKLLSQELGRKPTEEEIATRMEITIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIESDGET
PEDRVAKVLLREDLESVLETLTARERDVLKLRYGLDDGRMKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEY
IR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=59.3360995850622, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789098, Length=236, Percent_Identity=43.6440677966102, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1789871, Length=250, Percent_Identity=28.4, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=31.6831683168317, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 46298; Mature: 46167

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAKELVKSGKTALLESDLENPPSGTLELDTETDTLESELDTGDTDAFDEDDDDEEAAE
CCHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCCCCHHHHHH
EDGETAAAGAKGRSKRKAASKKKPYTDDSIRVYLQEIGRIRLLRADEEIELARKIADLLE
HCCCHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHH
LERAREAVFQRMGLWSDPEEVPDTLWAEEVKMSLPEFRRRLHRGRRAKEKMVQSNLRLVV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSR
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
TIRLPVHLYETISRIKKVTKLLSQELGRKPTEEEIATRMEITIEKLRFIAKSAQLPISLE
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
TPIGKEEDSRLGDFIESDGETPEDRVAKVLLREDLESVLETLTARERDVLKLRYGLDDGR
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
>Mature Secondary Structure 
AQAKELVKSGKTALLESDLENPPSGTLELDTETDTLESELDTGDTDAFDEDDDDEEAAE
CHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCCCCHHHHHH
EDGETAAAGAKGRSKRKAASKKKPYTDDSIRVYLQEIGRIRLLRADEEIELARKIADLLE
HCCCHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHH
LERAREAVFQRMGLWSDPEEVPDTLWAEEVKMSLPEFRRRLHRGRRAKEKMVQSNLRLVV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIAKKYMNRGLSFQDLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSR
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
TIRLPVHLYETISRIKKVTKLLSQELGRKPTEEEIATRMEITIEKLRFIAKSAQLPISLE
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
TPIGKEEDSRLGDFIESDGETPEDRVAKVLLREDLESVLETLTARERDVLKLRYGLDDGR
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MKTLEEIGQIFNVTRERIRQIEAKALRKLRHPNRNSVLKEYIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]