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The map label for this gene is cobS [H]

Identifier: 86609650

GI number: 86609650

Start: 2310599

End: 2311405

Strand: Reverse

Name: cobS [H]

Synonym: CYB_2204

Alternate gene names: 86609650

Gene position: 2311405-2310599 (Counterclockwise)

Preceding gene: 86609651

Following gene: 86609649

Centisome position: 75.87

GC content: 62.45

Gene sequence:

>807_bases
TTGCGGCGCTTCTGGGCAGCCTTGACTTTCTACACGGCCCTGCCTCTGGGGTGGGTCGGAGACTTGCCGTTTGAGGGCAT
TGCCGCCTGGCTGCCAGGGGTAGGTGTGTTGCTGGGCGGGATCCTCTCCCTGGCTGGGATCCTGCTGCAGATTTTCCCCA
ATCCGGTGCGGGCCGCTTTGCTCTTGGCCCTGTGGGTTCTCTTGACCGGTGGGCTACACCTGGACGGGGTGGCCGATACT
GCCGATGGCTTGGCGATCAATGTCCACAAGAATCCTTACAAAGGGGATCCGGAACGGTCAACTGAGGATGGACAAAAATC
TGACTTGCAGGGATCCCGTCGCCGTCTGCAGGTGATGAGCGATAGCCACACCGGGGCCTATGGGGTACTGGCCTTGGTGC
TGCTGCTGCTGCTGAAATTTGCTGCCCTGAGCAGCGTTCGAGCTTGGCCCCTCTTGCTGCTGGTGCCCGCCTGGGGGCGT
TGGGGCCAGTTGCTCGCCATTGCCCTGTACCCCTATCTGCGAGAGCAGGGATCCGCCCGCTTCCTCAAAGACTCCACTTC
CCTGCCGGGATCCCTGTGGCCGGGCACTCTGCTTGTGCTGGCCATCACCGGGGGGTTGGGCTGGGCTGGGATCTTGGCTT
GGCCCCCTTTGCTGCTCTGGACGCTGGGATCCGCCCTGGGGGCTTGGGCCGTGGGCTACTGGATCTACCGCCAGTTGGGA
GGACACACGGGCGATACCTACGGAGCTACGGTGGAATGGTCAGAAGCGATAGCATTGCTGTGGGGATCCCTTATTGGGCC
ATCATGA

Upstream 100 bases:

>100_bases
TCACCATTGTGGTGGGGCGCACCGTTCGCAAGGATTCCTATTTTCTCTCCCAGAGGGCTGGATTGGCGGATCCCTTTACA
CAGACTCAAGGACTGAGCGA

Downstream 100 bases:

>100_bases
ATCTGATACATTTACGCTGAACACTGCTGAACATCACTGTTCAACTATGCAGCGATAGCATTCTGTTTGTTTCCATTCGC
GTTAGCGGGACGGAGTCCAA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRRFWAALTFYTALPLGWVGDLPFEGIAAWLPGVGVLLGGILSLAGILLQIFPNPVRAALLLALWVLLTGGLHLDGVADT
ADGLAINVHKNPYKGDPERSTEDGQKSDLQGSRRRLQVMSDSHTGAYGVLALVLLLLLKFAALSSVRAWPLLLLVPAWGR
WGQLLAIALYPYLREQGSARFLKDSTSLPGSLWPGTLLVLAITGGLGWAGILAWPPLLLWTLGSALGAWAVGYWIYRQLG
GHTGDTYGATVEWSEAIALLWGSLIGPS

Sequences:

>Translated_268_residues
MRRFWAALTFYTALPLGWVGDLPFEGIAAWLPGVGVLLGGILSLAGILLQIFPNPVRAALLLALWVLLTGGLHLDGVADT
ADGLAINVHKNPYKGDPERSTEDGQKSDLQGSRRRLQVMSDSHTGAYGVLALVLLLLLKFAALSSVRAWPLLLLVPAWGR
WGQLLAIALYPYLREQGSARFLKDSTSLPGSLWPGTLLVLAITGGLGWAGILAWPPLLLWTLGSALGAWAVGYWIYRQLG
GHTGDTYGATVEWSEAIALLWGSLIGPS
>Mature_268_residues
MRRFWAALTFYTALPLGWVGDLPFEGIAAWLPGVGVLLGGILSLAGILLQIFPNPVRAALLLALWVLLTGGLHLDGVADT
ADGLAINVHKNPYKGDPERSTEDGQKSDLQGSRRRLQVMSDSHTGAYGVLALVLLLLLKFAALSSVRAWPLLLLVPAWGR
WGQLLAIALYPYLREQGSARFLKDSTSLPGSLWPGTLLVLAITGGLGWAGILAWPPLLLWTLGSALGAWAVGYWIYRQLG
GHTGDTYGATVEWSEAIALLWGSLIGPS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=274, Percent_Identity=32.4817518248175, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28740; Mature: 28740

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFWAALTFYTALPLGWVGDLPFEGIAAWLPGVGVLLGGILSLAGILLQIFPNPVRAAL
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LLALWVLLTGGLHLDGVADTADGLAINVHKNPYKGDPERSTEDGQKSDLQGSRRRLQVMS
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DSHTGAYGVLALVLLLLLKFAALSSVRAWPLLLLVPAWGRWGQLLAIALYPYLREQGSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCH
FLKDSTSLPGSLWPGTLLVLAITGGLGWAGILAWPPLLLWTLGSALGAWAVGYWIYRQLG
HHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHTGDTYGATVEWSEAIALLWGSLIGPS
CCCCCCCCCEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRFWAALTFYTALPLGWVGDLPFEGIAAWLPGVGVLLGGILSLAGILLQIFPNPVRAAL
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LLALWVLLTGGLHLDGVADTADGLAINVHKNPYKGDPERSTEDGQKSDLQGSRRRLQVMS
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DSHTGAYGVLALVLLLLLKFAALSSVRAWPLLLLVPAWGRWGQLLAIALYPYLREQGSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCH
FLKDSTSLPGSLWPGTLLVLAITGGLGWAGILAWPPLLLWTLGSALGAWAVGYWIYRQLG
HHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHTGDTYGATVEWSEAIALLWGSLIGPS
CCCCCCCCCEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA