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The map label for this gene is cobQ

Identifier: 86609619

GI number: 86609619

Start: 2275713

End: 2277212

Strand: Direct

Name: cobQ

Synonym: CYB_2173

Alternate gene names: 86609619

Gene position: 2275713-2277212 (Clockwise)

Preceding gene: 86609618

Following gene: 86609626

Centisome position: 74.69

GC content: 60.53

Gene sequence:

>1500_bases
GTGAGCTGTTCAGGTCGATCCTTGATGGTGGTGGGAACTTCTTCCCATGTGGGCAAAACCCTTTTGGTGACAGCCCTTTG
TCGCATTCTCAAGAAAGCGGGAAAAAGGGTTGCCCCTTTCAAAGCTCAGAACATGTCCCTCAACGCCTATGTCACCCCAG
ACGGCAAAGAGATCGCCTACGCGCAGGCTCTGCAAGCTTGGGCTGCCGGGATCCCCCCTGCGGTGGAAATGAACCCGATT
CTTCTGAAGCCCCAGGGCAACCTGACTTCCCAGATCGTCTTGAACGGAGTGGCGGTGGGCACCTACCGCGCCGGAGAGTA
TTACGAGCGCTGGTTTGCGCCGGCCTGGCAAGCGGTCAAAGAAGCCCTTGCTCGCCTGCAAAAGCAGTACGACTGGATCC
TTTGCGAGGGGGCAGGTAGCCCAGCAGAGGTGAACCTCAAACATCGGGACTTGGCAAATATGCGGGTTGCCTTGCATTTG
GGATCCCCCACTTGGCTGGTGGCGGACATCGACCGAGGTGGGGCGCTGGCCCATGTGGTGGGCACACTCCAACTGCTGGA
ACCCGAAGAACGGGCCCTGATCCGGGGCATTGTCATCAACAAGTTTCGGGGCTCGCGGGAACTGTTGCAGCCCGGCCTGG
ATTGGCTGGAAAACTACACCGGGATCCCAGTTGTCGGGGTCTTGCCTTGGGTGGACTGGGTTCTGCCCCAAGAGGATTCG
ATGGGAATTGCAGCCAACCCCCTGCTTTGGGAAGATCGCCGCGACCGAGCCGGTGGCCAAGCCCTGAGGGAAGGGCGCCT
GGAGATTGCTGTGGTGCGTTTGCCCCAGGTGGCCAATTTCTCGGATTTTGACCCCTTGTTGGCGGAGCCCACCGTCCATC
TGCGCTGGGTTCACCCCGGCCAAAGTTTGGGATCCCCGGATGTGGTCATCCTGCCCGGCAGCAAAACCACCCTCAACGAT
CTGTTCGCGTTGCAAAAGACCGGCTTGGCCGAGCAACTGCGCCAATACTCTGGCCATATCGTCGGCATTTGCGGCGGACT
GCAAATGTTGGGAGAAACCATCGCCGATCCCGAAGGCTGGGAGGGGATCGCCGGCACCTACTCGGGGTTGGGCTTTCTCC
CTTTGACCACCGTTTTACAGCCCACCAAGGTCACCCAGCAGGTACAGACCCAAAGCCGATGGCCTGCCCTGGCCCCCATC
CAGGGGTACGAAATTCATCAGGGATCCACCCAGGCGGATCCCTCTGGCTGCCTGCCGCTGTTCGACCGGGAGAACCTGGG
CTGGCGGGATCCCACGGGTCGGATTTGGGGCAGCTATCTGCATGGCCTGTTTGACAACCACCTGTGGCGGCGACAATGGC
TGAACTGGCTGCGCCGACAAAAAGGCTGGGATCCCTTGCCGGAGCTGGAGGGCCACTATGCTCAGCAGCGGGAGCAATTG
CTGGAGCGCCTGGCCGATCTGTGGCAGCCTCACCTGGACTTGGGCCTGTTGATGGAATAG

Upstream 100 bases:

>100_bases
GGGGATTGCAGAGTCGCTTTTGCCAACGGAGGCCCTGGCTTGGCAGGACTGGCCGCGGGGAGAATGGCAACAACCAGAAA
CCAAGGGCGAGGGATCCGGG

Downstream 100 bases:

>100_bases
CCGCTCCGAAAGACTTATGCAACGCTGAACAAAACAGAACTTTGCCCAGCATCGCTGCTTTCTTCCTGCTTCAGCGACCC
ATGACTGGCGGCAGAGTTCA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSCSGRSLMVVGTSSHVGKTLLVTALCRILKKAGKRVAPFKAQNMSLNAYVTPDGKEIAYAQALQAWAAGIPPAVEMNPI
LLKPQGNLTSQIVLNGVAVGTYRAGEYYERWFAPAWQAVKEALARLQKQYDWILCEGAGSPAEVNLKHRDLANMRVALHL
GSPTWLVADIDRGGALAHVVGTLQLLEPEERALIRGIVINKFRGSRELLQPGLDWLENYTGIPVVGVLPWVDWVLPQEDS
MGIAANPLLWEDRRDRAGGQALREGRLEIAVVRLPQVANFSDFDPLLAEPTVHLRWVHPGQSLGSPDVVILPGSKTTLND
LFALQKTGLAEQLRQYSGHIVGICGGLQMLGETIADPEGWEGIAGTYSGLGFLPLTTVLQPTKVTQQVQTQSRWPALAPI
QGYEIHQGSTQADPSGCLPLFDRENLGWRDPTGRIWGSYLHGLFDNHLWRRQWLNWLRRQKGWDPLPELEGHYAQQREQL
LERLADLWQPHLDLGLLME

Sequences:

>Translated_499_residues
MSCSGRSLMVVGTSSHVGKTLLVTALCRILKKAGKRVAPFKAQNMSLNAYVTPDGKEIAYAQALQAWAAGIPPAVEMNPI
LLKPQGNLTSQIVLNGVAVGTYRAGEYYERWFAPAWQAVKEALARLQKQYDWILCEGAGSPAEVNLKHRDLANMRVALHL
GSPTWLVADIDRGGALAHVVGTLQLLEPEERALIRGIVINKFRGSRELLQPGLDWLENYTGIPVVGVLPWVDWVLPQEDS
MGIAANPLLWEDRRDRAGGQALREGRLEIAVVRLPQVANFSDFDPLLAEPTVHLRWVHPGQSLGSPDVVILPGSKTTLND
LFALQKTGLAEQLRQYSGHIVGICGGLQMLGETIADPEGWEGIAGTYSGLGFLPLTTVLQPTKVTQQVQTQSRWPALAPI
QGYEIHQGSTQADPSGCLPLFDRENLGWRDPTGRIWGSYLHGLFDNHLWRRQWLNWLRRQKGWDPLPELEGHYAQQREQL
LERLADLWQPHLDLGLLME
>Mature_498_residues
SCSGRSLMVVGTSSHVGKTLLVTALCRILKKAGKRVAPFKAQNMSLNAYVTPDGKEIAYAQALQAWAAGIPPAVEMNPIL
LKPQGNLTSQIVLNGVAVGTYRAGEYYERWFAPAWQAVKEALARLQKQYDWILCEGAGSPAEVNLKHRDLANMRVALHLG
SPTWLVADIDRGGALAHVVGTLQLLEPEERALIRGIVINKFRGSRELLQPGLDWLENYTGIPVVGVLPWVDWVLPQEDSM
GIAANPLLWEDRRDRAGGQALREGRLEIAVVRLPQVANFSDFDPLLAEPTVHLRWVHPGQSLGSPDVVILPGSKTTLNDL
FALQKTGLAEQLRQYSGHIVGICGGLQMLGETIADPEGWEGIAGTYSGLGFLPLTTVLQPTKVTQQVQTQSRWPALAPIQ
GYEIHQGSTQADPSGCLPLFDRENLGWRDPTGRIWGSYLHGLFDNHLWRRQWLNWLRRQKGWDPLPELEGHYAQQREQLL
ERLADLWQPHLDLGLLME

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_SYNJB (Q2JJP4)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478381.1
- ProteinModelPortal:   Q2JJP4
- STRING:   Q2JJP4
- GeneID:   3902875
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2173
- TIGR:   CYB_2173
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   SSP321332:CYB_2173-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR003593
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- SMART:   SM00382
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 55355; Mature: 55224

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 344-344 ACT_SITE 441-441

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCSGRSLMVVGTSSHVGKTLLVTALCRILKKAGKRVAPFKAQNMSLNAYVTPDGKEIAY
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHH
AQALQAWAAGIPPAVEMNPILLKPQGNLTSQIVLNGVAVGTYRAGEYYERWFAPAWQAVK
HHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHEEEEEEEEEEECHHHHHHHHHHHHHHHH
EALARLQKQYDWILCEGAGSPAEVNLKHRDLANMRVALHLGSPTWLVADIDRGGALAHVV
HHHHHHHHCCCEEEEECCCCCCEEEEHHHCCCCEEEEEEECCCEEEEEECCCCCHHHHHH
GTLQLLEPEERALIRGIVINKFRGSRELLQPGLDWLENYTGIPVVGVLPWVDWVLPQEDS
HHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
MGIAANPLLWEDRRDRAGGQALREGRLEIAVVRLPQVANFSDFDPLLAEPTVHLRWVHPG
CCCCCCCCEECCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCHHCCCCEEEEEECCC
QSLGSPDVVILPGSKTTLNDLFALQKTGLAEQLRQYSGHIVGICGGLQMLGETIADPEGW
CCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCC
EGIAGTYSGLGFLPLTTVLQPTKVTQQVQTQSRWPALAPIQGYEIHQGSTQADPSGCLPL
CCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCC
FDRENLGWRDPTGRIWGSYLHGLFDNHLWRRQWLNWLRRQKGWDPLPELEGHYAQQREQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHH
LERLADLWQPHLDLGLLME
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SCSGRSLMVVGTSSHVGKTLLVTALCRILKKAGKRVAPFKAQNMSLNAYVTPDGKEIAY
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHH
AQALQAWAAGIPPAVEMNPILLKPQGNLTSQIVLNGVAVGTYRAGEYYERWFAPAWQAVK
HHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHEEEEEEEEEEECHHHHHHHHHHHHHHHH
EALARLQKQYDWILCEGAGSPAEVNLKHRDLANMRVALHLGSPTWLVADIDRGGALAHVV
HHHHHHHHCCCEEEEECCCCCCEEEEHHHCCCCEEEEEEECCCEEEEEECCCCCHHHHHH
GTLQLLEPEERALIRGIVINKFRGSRELLQPGLDWLENYTGIPVVGVLPWVDWVLPQEDS
HHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
MGIAANPLLWEDRRDRAGGQALREGRLEIAVVRLPQVANFSDFDPLLAEPTVHLRWVHPG
CCCCCCCCEECCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCHHCCCCEEEEEECCC
QSLGSPDVVILPGSKTTLNDLFALQKTGLAEQLRQYSGHIVGICGGLQMLGETIADPEGW
CCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCC
EGIAGTYSGLGFLPLTTVLQPTKVTQQVQTQSRWPALAPIQGYEIHQGSTQADPSGCLPL
CCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCC
FDRENLGWRDPTGRIWGSYLHGLFDNHLWRRQWLNWLRRQKGWDPLPELEGHYAQQREQL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHH
LERLADLWQPHLDLGLLME
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA