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The map label for this gene is trmB

Identifier: 86609613

GI number: 86609613

Start: 2269265

End: 2269984

Strand: Direct

Name: trmB

Synonym: CYB_2166

Alternate gene names: 86609613

Gene position: 2269265-2269984 (Clockwise)

Preceding gene: 86609612

Following gene: 86609614

Centisome position: 74.48

GC content: 60.69

Gene sequence:

>720_bases
ATGGAAGCAGAGGTGCAACAGGGGCAGCAATCGCCTGAGGGCCAACTGGAGAAGCGCCCCCCTTCTCCGCCTTGGGCCGG
GATCCCCTTACAGAGGGGCCTCGGTCACCGGGTACGGCAGCACGTCAATCCCCTGCAGGCACAGTATCAGCAGCCCGCCC
CCCCACCCCATTGGGAACGGGTATACCGGCGGCTGGGGCAGCCCTTTCATCTGGATATCGGCACAGGGAGCGGACGATTT
CTGCTGCGCATTGCTCAGGAGCAGCCCGACTGGAACTTCCTGGGGGTAGAGATTCGGCAGGCGCTGGTGGAACGGGCCAA
TGCCTGGCGGGACGAGCTGGGGCTGGATAACGTCCATTTTCTGTTTGCCAACATCAATGTCTCTTTGCGGCATCTGTTTG
CGCCGGGGGATCTCAGCCGGGTGACCATCCTATTTCCGGATCCCTGGTTCAAAAAGCGCCATCACAAACGGCGGATTGTC
CAACCCCAACTGGTGGCAGATCTGGCTCTGCTGCTGCGACCGGGCAGCCCTGTCTTCCTGCAATCGGATATCCAAGAGGT
GGCCGAAGAAATGGCAGCTCGATTTTTAGAGCATCCGCAGTTTTGGGATCCGCACCAGGGGCCTTTGGACTCCAATCCCC
TCGGCATCCCCACGGAGCGGGAGTGGCAGTGTCTGCAATTGGATCTGCCCATCTATCGCTATTGGCTGGAGCGACGGTGA

Upstream 100 bases:

>100_bases
GGTGGTAGCCTAGATCTCCTGGACTTTTGTCGCTCCCCTCCAAGCTGGGGGATCCCTTGGTCAGTGGGCGAGGGCCGCAG
CTGGCGGCAGGAGGTGGTCT

Downstream 100 bases:

>100_bases
CAACAGGACAAATCCGTAATGCAGTAATAACAACTCCATTCCGCCAAGACAATACAGAAGATAGGATATAACCCGCTTGA
GTTGGATCCGTTGCCGCAAT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEAEVQQGQQSPEGQLEKRPPSPPWAGIPLQRGLGHRVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRF
LLRIAQEQPDWNFLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFAPGDLSRVTILFPDPWFKKRHHKRRIV
QPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARFLEHPQFWDPHQGPLDSNPLGIPTEREWQCLQLDLPIYRYWLERR

Sequences:

>Translated_239_residues
MEAEVQQGQQSPEGQLEKRPPSPPWAGIPLQRGLGHRVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRF
LLRIAQEQPDWNFLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFAPGDLSRVTILFPDPWFKKRHHKRRIV
QPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARFLEHPQFWDPHQGPLDSNPLGIPTEREWQCLQLDLPIYRYWLERR
>Mature_239_residues
MEAEVQQGQQSPEGQLEKRPPSPPWAGIPLQRGLGHRVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRF
LLRIAQEQPDWNFLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFAPGDLSRVTILFPDPWFKKRHHKRRIV
QPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARFLEHPQFWDPHQGPLDSNPLGIPTEREWQCLQLDLPIYRYWLERR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Homo sapiens, GI167004078, Length=165, Percent_Identity=35.1515151515151, Blast_Score=85, Evalue=5e-17,
Organism=Escherichia coli, GI1789330, Length=124, Percent_Identity=33.8709677419355, Blast_Score=71, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17536689, Length=203, Percent_Identity=32.512315270936, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320000, Length=194, Percent_Identity=29.8969072164948, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI20128915, Length=224, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_SYNJB (Q2JJQ0)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478375.1
- ProteinModelPortal:   Q2JJQ0
- SMR:   Q2JJQ0
- STRING:   Q2JJQ0
- GeneID:   3902599
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2166
- TIGR:   CYB_2166
- eggNOG:   COG0220
- HOGENOM:   HBG284653
- OMA:   KRRVVQP
- PhylomeDB:   Q2JJQ0
- ProtClustDB:   CLSK750734
- BioCyc:   SSP321332:CYB_2166-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27864; Mature: 27864

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: BINDING 72-72 BINDING 97-97 BINDING 124-124 BINDING 147-147 BINDING 151-151 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAEVQQGQQSPEGQLEKRPPSPPWAGIPLQRGLGHRVRQHVNPLQAQYQQPAPPPHWER
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHH
VYRRLGQPFHLDIGTGSGRFLLRIAQEQPDWNFLGVEIRQALVERANAWRDELGLDNVHF
HHHHCCCCEEEEECCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEE
LFANINVSLRHLFAPGDLSRVTILFPDPWFKKRHHKRRIVQPQLVADLALLLRPGSPVFL
EEEECCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEE
QSDIQEVAEEMAARFLEHPQFWDPHQGPLDSNPLGIPTEREWQCLQLDLPIYRYWLERR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCC
>Mature Secondary Structure
MEAEVQQGQQSPEGQLEKRPPSPPWAGIPLQRGLGHRVRQHVNPLQAQYQQPAPPPHWER
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHH
VYRRLGQPFHLDIGTGSGRFLLRIAQEQPDWNFLGVEIRQALVERANAWRDELGLDNVHF
HHHHCCCCEEEEECCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEE
LFANINVSLRHLFAPGDLSRVTILFPDPWFKKRHHKRRIVQPQLVADLALLLRPGSPVFL
EEEECCEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEE
QSDIQEVAEEMAARFLEHPQFWDPHQGPLDSNPLGIPTEREWQCLQLDLPIYRYWLERR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA