Click here to switch to the map view.

The map label for this gene is ddlB

Identifier: 86609556

GI number: 86609556

Start: 2202565

End: 2203542

Strand: Reverse

Name: ddlB

Synonym: CYB_2109

Alternate gene names: 86609556

Gene position: 2203542-2202565 (Counterclockwise)

Preceding gene: 86609557

Following gene: 86609554

Centisome position: 72.33

GC content: 61.15

Gene sequence:

>978_bases
ATGAACATTGTTGTCTTGGCGGGGGGGCGTTCGGCAGAACGTCAAGTCTCTTGGGTAACGGGTAAAGCCTGCAAACGGGC
TTTGGAGGACCTGGGCCATCGGGTCAAGGTAATCGACCCAGATGAGGATCTGCCTCTGCGCCTTTGGCAGGAACGGCAAG
CCGGCTGCGACTTCGTTTGGATTGCCTTGCATGGCCCTGGTGGCGAGGATGGGGTGGTACAGGGAATGCTGGACTGGCTG
GGCCTGCCCTACCAGGGATCCGGGCCGCTGGCCAGTGCTCTGGCCATGGATAAGCTGGTCTCCAAGCAGATCTTTCGCGC
CGCGGGGATCCCTACCCCCGATTGGCAGGTTTGGGATGACCAAAACCCCCTCACTTGGGCCGACTGTGCGGCAGAGTTGG
GATCCCCACTGGTGATTAAGCCCAGCAACAACGGCTCGACGGTGGGCATCAGCATTGTCCGGGATGAGAGGTCATTTGCT
CAGGGGCTGGAGTTGGCCCGCTCGGTCTCGTCGCGGATTTTTCTGGAGCGCTATGTTCCCGGAAAGGAGATCACCCTCTC
GATCTTGTCCGGCCAAGTTCTTCCGGCCATTGAGATCATCCCCGCCCAAGGCGACTTCTACGACTACGAAGCCAAGTACG
CACCCGGTGGCTCGCGGCATTTGATCCCCTGTTCCCTCAGCCCGGCAGGGTTGGCCCGCTGTGAGGCGGCAGGTCTGAAG
GCCTACCAGGCGTTGGGGTGCGAAGGCTTGGCGCGGGTGGATCTGCGGGTGGATCCCGAGGAAAATCCTTGGGTGTTGGA
GGTGAATACACTGCCCGGCATGACCCCCACTTCCCTCTGTCCCGATGCGGCGGCAGCTCTGGGGTGGACGTTCACCGAGT
TGGTGGAGCGAATGCTGCAGGATGCCTTGCAAAAAGCCCAATTGACCGGATCCCGGCATCCTCAAAGTTCATCGGCGACT
CTCGGCAACGCTCCCTGA

Upstream 100 bases:

>100_bases
GTTCTTGCGCCGCCGACGGCCCCCTTCGTAGGGTAGGGTGAAGGTATGCTGGTATCTTGGTGCCGGTTGTTGAGGGTGGG
GAGTGCTTTGGGGTTAGGCC

Downstream 100 bases:

>100_bases
GGCTTTTCCCAAGACAAGAACAAGAACCTTTACCCAGCTCTTTCAGAGAAGGGCAACCAGCAAAAGAATCACCCCAATTC
CAACAATCAGTACAGCTCCC

Product: D-alanine--D-alanine ligase B

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNIVVLAGGRSAERQVSWVTGKACKRALEDLGHRVKVIDPDEDLPLRLWQERQAGCDFVWIALHGPGGEDGVVQGMLDWL
GLPYQGSGPLASALAMDKLVSKQIFRAAGIPTPDWQVWDDQNPLTWADCAAELGSPLVIKPSNNGSTVGISIVRDERSFA
QGLELARSVSSRIFLERYVPGKEITLSILSGQVLPAIEIIPAQGDFYDYEAKYAPGGSRHLIPCSLSPAGLARCEAAGLK
AYQALGCEGLARVDLRVDPEENPWVLEVNTLPGMTPTSLCPDAAAALGWTFTELVERMLQDALQKAQLTGSRHPQSSSAT
LGNAP

Sequences:

>Translated_325_residues
MNIVVLAGGRSAERQVSWVTGKACKRALEDLGHRVKVIDPDEDLPLRLWQERQAGCDFVWIALHGPGGEDGVVQGMLDWL
GLPYQGSGPLASALAMDKLVSKQIFRAAGIPTPDWQVWDDQNPLTWADCAAELGSPLVIKPSNNGSTVGISIVRDERSFA
QGLELARSVSSRIFLERYVPGKEITLSILSGQVLPAIEIIPAQGDFYDYEAKYAPGGSRHLIPCSLSPAGLARCEAAGLK
AYQALGCEGLARVDLRVDPEENPWVLEVNTLPGMTPTSLCPDAAAALGWTFTELVERMLQDALQKAQLTGSRHPQSSSAT
LGNAP
>Mature_325_residues
MNIVVLAGGRSAERQVSWVTGKACKRALEDLGHRVKVIDPDEDLPLRLWQERQAGCDFVWIALHGPGGEDGVVQGMLDWL
GLPYQGSGPLASALAMDKLVSKQIFRAAGIPTPDWQVWDDQNPLTWADCAAELGSPLVIKPSNNGSTVGISIVRDERSFA
QGLELARSVSSRIFLERYVPGKEITLSILSGQVLPAIEIIPAQGDFYDYEAKYAPGGSRHLIPCSLSPAGLARCEAAGLK
AYQALGCEGLARVDLRVDPEENPWVLEVNTLPGMTPTSLCPDAAAALGWTFTELVERMLQDALQKAQLTGSRHPQSSSAT
LGNAP

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=41.8300653594771, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786579, Length=279, Percent_Identity=34.7670250896057, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_SYNJB (Q2JJV2)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_478318.1
- ProteinModelPortal:   Q2JJV2
- SMR:   Q2JJV2
- STRING:   Q2JJV2
- GeneID:   3902696
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_2109
- TIGR:   CYB_2109
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q2JJV2
- ProtClustDB:   CLSK494701
- BioCyc:   SSP321332:CYB_2109-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 34949; Mature: 34949

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVVLAGGRSAERQVSWVTGKACKRALEDLGHRVKVIDPDEDLPLRLWQERQAGCDFVW
CEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCEEE
IALHGPGGEDGVVQGMLDWLGLPYQGSGPLASALAMDKLVSKQIFRAAGIPTPDWQVWDD
EEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
QNPLTWADCAAELGSPLVIKPSNNGSTVGISIVRDERSFAQGLELARSVSSRIFLERYVP
CCCCCHHHHHHHHCCCEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCC
GKEITLSILSGQVLPAIEIIPAQGDFYDYEAKYAPGGSRHLIPCSLSPAGLARCEAAGLK
CCCEEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHH
AYQALGCEGLARVDLRVDPEENPWVLEVNTLPGMTPTSLCPDAAAALGWTFTELVERMLQ
HHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCCCHHCCCHHHHHHCCHHHHHHHHHHH
DALQKAQLTGSRHPQSSSATLGNAP
HHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIVVLAGGRSAERQVSWVTGKACKRALEDLGHRVKVIDPDEDLPLRLWQERQAGCDFVW
CEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCEEE
IALHGPGGEDGVVQGMLDWLGLPYQGSGPLASALAMDKLVSKQIFRAAGIPTPDWQVWDD
EEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
QNPLTWADCAAELGSPLVIKPSNNGSTVGISIVRDERSFAQGLELARSVSSRIFLERYVP
CCCCCHHHHHHHHCCCEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCC
GKEITLSILSGQVLPAIEIIPAQGDFYDYEAKYAPGGSRHLIPCSLSPAGLARCEAAGLK
CCCEEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHHH
AYQALGCEGLARVDLRVDPEENPWVLEVNTLPGMTPTSLCPDAAAALGWTFTELVERMLQ
HHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCCCCHHCCCHHHHHHCCHHHHHHHHHHH
DALQKAQLTGSRHPQSSSATLGNAP
HHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA