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The map label for this gene is rimO [H]

Identifier: 86609485

GI number: 86609485

Start: 2138247

End: 2139731

Strand: Direct

Name: rimO [H]

Synonym: CYB_2037

Alternate gene names: 86609485

Gene position: 2138247-2139731 (Clockwise)

Preceding gene: 86609484

Following gene: 86609486

Centisome position: 70.18

GC content: 60.81

Gene sequence:

>1485_bases
ATGCGGCCCTCAACTAGAATAGATAAGCCTCTGGCAAGCTGCCCCCTTTTTGATGCTCAGTCCATGAATGGGTTAGAAGC
GACCGTCTCCAGTTTCCCATCCGTTAAGGACTCCGTCCCGCTTACGCGAACAGCGGTGCAGCGTACAAAGGCGTCGGTTG
CCGTTTTACACCTGGGCTGCGAGAAAAACCGGGTGGACACCGAGCACATGCTGGGCCTGCTGGCCCAAGCGGGCTACCGG
GTGGATGGGGACGAAGAGAGCGCTAACTATGTGATTGTCAACACCTGTAGCTTCATCGAGGCAGCGCGACGGGAGTCGGT
CAGTACCCTGATGGAGCTGGCGGTACAAGGCAAAAAGATCATCATTGCCGGCTGCTTGGCGCAACACTTCCAGGAGGAGC
TGCTGCAGGAGATCCCGGAGGCGGTGGCTATCGTGGGTACGGGCGACTATCACCAGATCGTCCAAATCATCGAGCGGGTG
GAGCGGGGAGAGCGGGTCAATGCCGTTACTTCTAGCCTGGATTACATTGCCGACGAGACCGTACCTCGCTATCGCACCAC
CCACGCGCCGGTGGCCTACCTGCGGGTAGCCGAGGGCTGTAACTACCGCTGCTCTTTCTGCATCATCCCCCACTTGCGGG
GCGACCAACGCTCTCGCTCCATCGAGTCGATCCTACGGGAAGCAGAACAGCTGGCCGAAGAGGGGGTGCAGGAGCTGATT
TTAATTTCCCAGATCACCACCCACTACGGGATCGATCTCTACGGGGAGCCGCGGCTGGCAGATCTCATTCGCGCCCTGGG
CAAGATCCCCATTCCCTGGATCCGCATGCTCTACGCCTACCCCACCGGCGTCACGCCTGCAGTGGTGGAGGCCATCCAAG
AAACTCCCAATTTCTTGCCTTACCTGGATTTGCCCCTACAGCACAGCCATCCGCAGATCCTCAAGGCCATGAATCGCCCC
TGGCAAGGGCAGGTCAACGACCGGGTGATCGAGCGGCTGCGCCAGGCGTTGCCCAATGCCGTGCTGCGCACCAGCTTTAT
CGTCGGCTTTCCTGGGGAGACGGAGGAGCACTTCCAGCATCTACTAGACTTTGTCCAGCGCCATCAGTTTGACCATGTGG
GGGTGTTTACCTTTTCTCCCGAAGAGGGCACCCCTGCCTACCACCTCCCCCATCAGGTTCCAGAGGCCGTCAAACAGGAG
CGCCGTGCCCGCCTGATGCAGGTGCAGCAGGGGATCACCTTCCGCCGCAACCGCGAGCAGGTAGGCCGGGTGGTTCCCGT
TCTCTTGGAGCAGGAAAACCCCAGAACCGGCGAGTGGATTGGCCGTTCCCCCCGCTTTGCCCCCGAGGTGGATGGAGTGG
TGTATGTGCAGGGGCAGGGATCCCTGGGATCCCTGGTGCCGGTGCAGATCACCCGCGCTGAGCCCTATGATCTCTTTGGC
CAAGTGGTGGCAGCGCCGGAGGGTTTTAGCTGGAGCGGGCGGTAG

Upstream 100 bases:

>100_bases
GGACGGGCACCTCTGTTTTCGACGGCGGCTGGCGGGGATCCCTACAGTTGGGCGGCCACCGCGTTGGCCGTGGCTACGGC
TCTATTGCTCCGCAACGCTG

Downstream 100 bases:

>100_bases
CGGTGCAAAGCACTTTGGTAGGATCTTCATCCACGGGAGAGGAGCTGGGAATGACTACCCTTCGCGTCAGCGGGACCGAG
TCTTTAGATGATCTGAAACA

Product: tRNA modifying protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRPSTRIDKPLASCPLFDAQSMNGLEATVSSFPSVKDSVPLTRTAVQRTKASVAVLHLGCEKNRVDTEHMLGLLAQAGYR
VDGDEESANYVIVNTCSFIEAARRESVSTLMELAVQGKKIIIAGCLAQHFQEELLQEIPEAVAIVGTGDYHQIVQIIERV
ERGERVNAVTSSLDYIADETVPRYRTTHAPVAYLRVAEGCNYRCSFCIIPHLRGDQRSRSIESILREAEQLAEEGVQELI
LISQITTHYGIDLYGEPRLADLIRALGKIPIPWIRMLYAYPTGVTPAVVEAIQETPNFLPYLDLPLQHSHPQILKAMNRP
WQGQVNDRVIERLRQALPNAVLRTSFIVGFPGETEEHFQHLLDFVQRHQFDHVGVFTFSPEEGTPAYHLPHQVPEAVKQE
RRARLMQVQQGITFRRNREQVGRVVPVLLEQENPRTGEWIGRSPRFAPEVDGVVYVQGQGSLGSLVPVQITRAEPYDLFG
QVVAAPEGFSWSGR

Sequences:

>Translated_494_residues
MRPSTRIDKPLASCPLFDAQSMNGLEATVSSFPSVKDSVPLTRTAVQRTKASVAVLHLGCEKNRVDTEHMLGLLAQAGYR
VDGDEESANYVIVNTCSFIEAARRESVSTLMELAVQGKKIIIAGCLAQHFQEELLQEIPEAVAIVGTGDYHQIVQIIERV
ERGERVNAVTSSLDYIADETVPRYRTTHAPVAYLRVAEGCNYRCSFCIIPHLRGDQRSRSIESILREAEQLAEEGVQELI
LISQITTHYGIDLYGEPRLADLIRALGKIPIPWIRMLYAYPTGVTPAVVEAIQETPNFLPYLDLPLQHSHPQILKAMNRP
WQGQVNDRVIERLRQALPNAVLRTSFIVGFPGETEEHFQHLLDFVQRHQFDHVGVFTFSPEEGTPAYHLPHQVPEAVKQE
RRARLMQVQQGITFRRNREQVGRVVPVLLEQENPRTGEWIGRSPRFAPEVDGVVYVQGQGSLGSLVPVQITRAEPYDLFG
QVVAAPEGFSWSGR
>Mature_494_residues
MRPSTRIDKPLASCPLFDAQSMNGLEATVSSFPSVKDSVPLTRTAVQRTKASVAVLHLGCEKNRVDTEHMLGLLAQAGYR
VDGDEESANYVIVNTCSFIEAARRESVSTLMELAVQGKKIIIAGCLAQHFQEELLQEIPEAVAIVGTGDYHQIVQIIERV
ERGERVNAVTSSLDYIADETVPRYRTTHAPVAYLRVAEGCNYRCSFCIIPHLRGDQRSRSIESILREAEQLAEEGVQELI
LISQITTHYGIDLYGEPRLADLIRALGKIPIPWIRMLYAYPTGVTPAVVEAIQETPNFLPYLDLPLQHSHPQILKAMNRP
WQGQVNDRVIERLRQALPNAVLRTSFIVGFPGETEEHFQHLLDFVQRHQFDHVGVFTFSPEEGTPAYHLPHQVPEAVKQE
RRARLMQVQQGITFRRNREQVGRVVPVLLEQENPRTGEWIGRSPRFAPEVDGVVYVQGQGSLGSLVPVQITRAEPYDLFG
QVVAAPEGFSWSGR

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=470, Percent_Identity=27.4468085106383, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI28872784, Length=421, Percent_Identity=28.0285035629454, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI93277076, Length=437, Percent_Identity=26.0869565217391, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=40.4921700223714, Blast_Score=310, Evalue=9e-86,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=31.6143497757848, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=488, Percent_Identity=25.2049180327869, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71996771, Length=371, Percent_Identity=23.989218328841, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356207, Length=422, Percent_Identity=28.9099526066351, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI19922432, Length=444, Percent_Identity=25.4504504504505, Blast_Score=148, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55378; Mature: 55378

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSTRIDKPLASCPLFDAQSMNGLEATVSSFPSVKDSVPLTRTAVQRTKASVAVLHLGC
CCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECC
EKNRVDTEHMLGLLAQAGYRVDGDEESANYVIVNTCSFIEAARRESVSTLMELAVQGKKI
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEE
IIAGCLAQHFQEELLQEIPEAVAIVGTGDYHQIVQIIERVERGERVNAVTSSLDYIADET
EEHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
VPRYRTTHAPVAYLRVAEGCNYRCSFCIIPHLRGDQRSRSIESILREAEQLAEEGVQELI
CCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LISQITTHYGIDLYGEPRLADLIRALGKIPIPWIRMLYAYPTGVTPAVVEAIQETPNFLP
HHHHHHHHCCCEECCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
YLDLPLQHSHPQILKAMNRPWQGQVNDRVIERLRQALPNAVLRTSFIVGFPGETEEHFQH
EECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHEEEECCCCCHHHHHH
LLDFVQRHQFDHVGVFTFSPEEGTPAYHLPHQVPEAVKQERRARLMQVQQGITFRRNREQ
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHH
VGRVVPVLLEQENPRTGEWIGRSPRFAPEVDGVVYVQGQGSLGSLVPVQITRAEPYDLFG
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHH
QVVAAPEGFSWSGR
HHHCCCCCCCCCCC
>Mature Secondary Structure
MRPSTRIDKPLASCPLFDAQSMNGLEATVSSFPSVKDSVPLTRTAVQRTKASVAVLHLGC
CCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECC
EKNRVDTEHMLGLLAQAGYRVDGDEESANYVIVNTCSFIEAARRESVSTLMELAVQGKKI
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEE
IIAGCLAQHFQEELLQEIPEAVAIVGTGDYHQIVQIIERVERGERVNAVTSSLDYIADET
EEHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
VPRYRTTHAPVAYLRVAEGCNYRCSFCIIPHLRGDQRSRSIESILREAEQLAEEGVQELI
CCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LISQITTHYGIDLYGEPRLADLIRALGKIPIPWIRMLYAYPTGVTPAVVEAIQETPNFLP
HHHHHHHHCCCEECCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
YLDLPLQHSHPQILKAMNRPWQGQVNDRVIERLRQALPNAVLRTSFIVGFPGETEEHFQH
EECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHEEEECCCCCHHHHHH
LLDFVQRHQFDHVGVFTFSPEEGTPAYHLPHQVPEAVKQERRARLMQVQQGITFRRNREQ
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCHHH
VGRVVPVLLEQENPRTGEWIGRSPRFAPEVDGVVYVQGQGSLGSLVPVQITRAEPYDLFG
HHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHH
QVVAAPEGFSWSGR
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA