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The map label for this gene is trmH [C]

Identifier: 86609412

GI number: 86609412

Start: 2058015

End: 2058827

Strand: Reverse

Name: trmH [C]

Synonym: CYB_1962

Alternate gene names: 86609412

Gene position: 2058827-2058015 (Counterclockwise)

Preceding gene: 86609413

Following gene: 86609410

Centisome position: 67.58

GC content: 62.98

Gene sequence:

>813_bases
ATGGCTTCTGAGCTGCTGACCAGTCCGAAAAACCCCTGGATCCGCAAGTTGCGGCAGTTGCACCGCAGCAAAGGCCGGCG
GGAACAGGGCGCCTTTTTGGTGGAGGGATCCCATCTCATCGAAGAAGCCCTGCGGGTCGGCTGGCCTTTGCAAGCCCTCT
GCTACACGCCCGACTGGGCCAGCCGTCACCCTCACCTGCTGGCCGATCTGCCCAAGGGCCTACGGCGGCAACCCGTCAGC
TCAGAAGTCATGGCAGCGCTCTGTACCACCGAGTCTCCCGAAGGAGCTGTGGCCGTGGCCGTCAGTTGGCAGATTTCCTC
TCCCCCCCTGTCCAAGTTACGTTTGGGCCTTGTGGTGGAAACGCTGCAAGATCCAGGCAATTTGGGATCCCTAATCCGAG
TCGTGGCCGCCGCAGGAGCCGACGGCCTTTGGCTAAGCGCAGACAGCGTCGATCCCGAAAACCCCAAAGTCTTGCGGGCC
TCTGCCGGCCAGTGGTTTCGCCGTCCGCCCCAAGTCGTGCCCGACCTAGCCGCCTGGATCCAGAGCTGCCGTTGCCACGG
GATCCAAGTCTTGGCTGCTACCCTCTCCCCCACAGCAAACTGCTACTGGGATCAAGATCTCACCCGCCCTACCCTTTTTC
TCCTGGGCAACGAAGGGGCTGGCCTGAGGGAAGCGACTATCCAACAGGCCGATGCCCAGGTGCGGATCCCGATGGCCGAG
GGTGTCGAATCTCTGAACGTCGCTATGACGGGGGGGTTGTTGCTCTACGAGGCCCTGCGCCAGCGGCGATCTCGCTCAGG
AAGCGCTCCCTGA

Upstream 100 bases:

>100_bases
TGCGCCGCTCCGGTTACTAACTCCCCTCTCCCTCTGGGAGAGGGGCTGGGGGTGAGGGCAGCAACGCCGAAGACTTGGGG
AGTACCTTAACCATTGCCAC

Downstream 100 bases:

>100_bases
ACAGCTAGCTGCTCCCTCAAAACTCTTCCTCTACAAAGACCGCATCCTCATTCTCTTCCTGCTCTCCCTCCTCCATCGCT
GCCGGCCGGCTGGCGGAAAC

Product: RNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MASELLTSPKNPWIRKLRQLHRSKGRREQGAFLVEGSHLIEEALRVGWPLQALCYTPDWASRHPHLLADLPKGLRRQPVS
SEVMAALCTTESPEGAVAVAVSWQISSPPLSKLRLGLVVETLQDPGNLGSLIRVVAAAGADGLWLSADSVDPENPKVLRA
SAGQWFRRPPQVVPDLAAWIQSCRCHGIQVLAATLSPTANCYWDQDLTRPTLFLLGNEGAGLREATIQQADAQVRIPMAE
GVESLNVAMTGGLLLYEALRQRRSRSGSAP

Sequences:

>Translated_270_residues
MASELLTSPKNPWIRKLRQLHRSKGRREQGAFLVEGSHLIEEALRVGWPLQALCYTPDWASRHPHLLADLPKGLRRQPVS
SEVMAALCTTESPEGAVAVAVSWQISSPPLSKLRLGLVVETLQDPGNLGSLIRVVAAAGADGLWLSADSVDPENPKVLRA
SAGQWFRRPPQVVPDLAAWIQSCRCHGIQVLAATLSPTANCYWDQDLTRPTLFLLGNEGAGLREATIQQADAQVRIPMAE
GVESLNVAMTGGLLLYEALRQRRSRSGSAP
>Mature_269_residues
ASELLTSPKNPWIRKLRQLHRSKGRREQGAFLVEGSHLIEEALRVGWPLQALCYTPDWASRHPHLLADLPKGLRRQPVSS
EVMAALCTTESPEGAVAVAVSWQISSPPLSKLRLGLVVETLQDPGNLGSLIRVVAAAGADGLWLSADSVDPENPKVLRAS
AGQWFRRPPQVVPDLAAWIQSCRCHGIQVLAATLSPTANCYWDQDLTRPTLFLLGNEGAGLREATIQQADAQVRIPMAEG
VESLNVAMTGGLLLYEALRQRRSRSGSAP

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=166, Percent_Identity=31.3253012048193, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790083, Length=155, Percent_Identity=32.258064516129, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790623, Length=146, Percent_Identity=29.4520547945205, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29355; Mature: 29224

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASELLTSPKNPWIRKLRQLHRSKGRREQGAFLVEGSHLIEEALRVGWPLQALCYTPDWA
CCCCHHCCCCCHHHHHHHHHHHHCCCHHCCCEEEECHHHHHHHHHCCCCHHEEEECCCCC
SRHPHLLADLPKGLRRQPVSSEVMAALCTTESPEGAVAVAVSWQISSPPLSKLRLGLVVE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
TLQDPGNLGSLIRVVAAAGADGLWLSADSVDPENPKVLRASAGQWFRRPPQVVPDLAAWI
HHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCHHHCCCCHHHHHHHHHH
QSCRCHGIQVLAATLSPTANCYWDQDLTRPTLFLLGNEGAGLREATIQQADAQVRIPMAE
HHHHHCCHHEEEECCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCHHH
GVESLNVAMTGGLLLYEALRQRRSRSGSAP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ASELLTSPKNPWIRKLRQLHRSKGRREQGAFLVEGSHLIEEALRVGWPLQALCYTPDWA
CCCHHCCCCCHHHHHHHHHHHHCCCHHCCCEEEECHHHHHHHHHCCCCHHEEEECCCCC
SRHPHLLADLPKGLRRQPVSSEVMAALCTTESPEGAVAVAVSWQISSPPLSKLRLGLVVE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
TLQDPGNLGSLIRVVAAAGADGLWLSADSVDPENPKVLRASAGQWFRRPPQVVPDLAAWI
HHHCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCHHHCCCCHHHHHHHHHH
QSCRCHGIQVLAATLSPTANCYWDQDLTRPTLFLLGNEGAGLREATIQQADAQVRIPMAE
HHHHHCCHHEEEECCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCHHH
GVESLNVAMTGGLLLYEALRQRRSRSGSAP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]