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The map label for this gene is prfC [H]

Identifier: 86609331

GI number: 86609331

Start: 1959185

End: 1960810

Strand: Reverse

Name: prfC [H]

Synonym: CYB_1875

Alternate gene names: 86609331

Gene position: 1960810-1959185 (Counterclockwise)

Preceding gene: 86609332

Following gene: 86609330

Centisome position: 64.36

GC content: 52.09

Gene sequence:

>1626_bases
ATGAAAGAACTTGATTTGGCCAAAGCTGTAGAGCAACGGCGCACCTTTGCCATTATTTCCCATCCCGATGCGGGGAAGAC
CACCCTCACCGAAAAGCTGCTGTTGTACGGGGGGGCCATTCAAGAAGCGGGAGCTGTGAAGGCCCGTCGGGCGCAAAAGC
GGGCCACTTCCGACTGGATGGAACTGGAAAAGCAGCGGGGTATTTCCATCACCTCTACCGTCCTGCAATTTGATTACCGC
AACATTCGTCTCAATCTGCTGGATACTCCCGGCCACCAGGACTTCAGCGAAGACACCTATCGCACCCTAGCAGCGGCTGA
CAATGCCGTGATGCTGGAAGATGCCGGCAAAGGGTTGGAAACCCAGACCCGCAAGCTGTTTGAAGTGTGTCAGATGCGGG
GGCTGCCGATTTTTACCTTTATCAACAAGATGGATCGCCCAAGTCGGGATCCCTTGGAGCTGATGGATGAAATTGAGACA
GAACTGGGCCTCAAGCCCTATCCTGTCAACTGGCCAATTGGCTCAGGCGAGCGGTTTACTGCGCTATTTGACCGCCGAAC
TCAGGAGTTTCATTTCTTTGAGCGGGTATCTGGGGGGCGGCTAAGAGTTCAAGATACCGTCATTAAACTTGGGGATCCGC
AAATTGAGGAGTTGATCGACAAGGACTTGTATTACCAATTCAAGGAAGAGCTGGAACTGCTGGATGGTCTCATGCCCACC
TTGGATCTAAAACAGGTGCATTCTGGCAAACAAACCCCGGTCTTTTTCGGCAGTGCCATGACCAATTTTGGGGTGGAGCT
TTTCCTCAATGCATTTTTGGAGTATTCCTTACCGCCAATGCCTCACGAGAGCACTGCTGGGCTAATCCCGCCCGAACATC
CTGAGTTTACGGGCTTCGTCTTTAAACTGCAGGCCAACATGGATCCCCGCCATCGGGATCGCGTTGCTTTTGTGCGCATC
TGTTCCGGCAAGTTTGAGAAGGATATGGTGGTCAACCATGCCCGCCTCAATCGTCCCATTCGCCTATCTTATCCCCAGAA
GTTGTTTGCTCAAGAGCGCATCTCCATCGATGAAGCTTACCCTGGGGATGTGATCGGCCTGAATAATCCCGGCATCTTCA
CCATTGGGGATACCATCTACACCGGCCCGAAGCTGCGCTACGCCGGGATCCCTAGCTTTTCTCCTGAGCTGTTTGCCTAC
TTGAAGAATCCCAATCCTTCCAAGTTTAAGCAGTTCCAGAAAGGGGTAGCGGAACTGCGGGAAGAAGGGGCTGTACAAAT
CATGTACTCTATCGACGATTCCAAACGGGATCCGATTGTGGCAGCGGTGGGCCAGTTGCAGTTTGAGGTTTTGCAGTATC
GCCTTGAAAGTGAATACAACGTCGAAACCCGACTGGAGATGTTGCCCTACACACTGGCTCGCTGGGTCGAGAATGGCTGG
AAGGCGCTGGAGAATATTCCCAGCTTGTTTAACACCCTAGTTGTTAAAGACATTCAAGACAGGCCCGTTCTTCTGTTTAA
GAACGAGTGGACTTTACAGCAATTTGCTGCCAGCAACCCTAAAATCAAGCTCAGCAAAATTGCCCCTATCTCGGATCTCG
GCCCAGAATTGGGTGACGGCCAATAG

Upstream 100 bases:

>100_bases
CTATTTTTTCCTTCTCCGTATTTCTCCCATCCTTTAGGGGACAACATCGCGGCGGAATCTTCTTTAAGGCGACCAGGTTG
TAGTGGAGCGGCAACAGTTG

Downstream 100 bases:

>100_bases
ATCAACCTAAGAGAATCCGTCTCAAAGAGCCTTAGGTTCTGACATTCAGTCGAGATGCCATTGGTCTCCACATCAGCGGT
GTTGATAGGTGTTGATATCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKELDLAKAVEQRRTFAIISHPDAGKTTLTEKLLLYGGAIQEAGAVKARRAQKRATSDWMELEKQRGISITSTVLQFDYR
NIRLNLLDTPGHQDFSEDTYRTLAAADNAVMLEDAGKGLETQTRKLFEVCQMRGLPIFTFINKMDRPSRDPLELMDEIET
ELGLKPYPVNWPIGSGERFTALFDRRTQEFHFFERVSGGRLRVQDTVIKLGDPQIEELIDKDLYYQFKEELELLDGLMPT
LDLKQVHSGKQTPVFFGSAMTNFGVELFLNAFLEYSLPPMPHESTAGLIPPEHPEFTGFVFKLQANMDPRHRDRVAFVRI
CSGKFEKDMVVNHARLNRPIRLSYPQKLFAQERISIDEAYPGDVIGLNNPGIFTIGDTIYTGPKLRYAGIPSFSPELFAY
LKNPNPSKFKQFQKGVAELREEGAVQIMYSIDDSKRDPIVAAVGQLQFEVLQYRLESEYNVETRLEMLPYTLARWVENGW
KALENIPSLFNTLVVKDIQDRPVLLFKNEWTLQQFAASNPKIKLSKIAPISDLGPELGDGQ

Sequences:

>Translated_541_residues
MKELDLAKAVEQRRTFAIISHPDAGKTTLTEKLLLYGGAIQEAGAVKARRAQKRATSDWMELEKQRGISITSTVLQFDYR
NIRLNLLDTPGHQDFSEDTYRTLAAADNAVMLEDAGKGLETQTRKLFEVCQMRGLPIFTFINKMDRPSRDPLELMDEIET
ELGLKPYPVNWPIGSGERFTALFDRRTQEFHFFERVSGGRLRVQDTVIKLGDPQIEELIDKDLYYQFKEELELLDGLMPT
LDLKQVHSGKQTPVFFGSAMTNFGVELFLNAFLEYSLPPMPHESTAGLIPPEHPEFTGFVFKLQANMDPRHRDRVAFVRI
CSGKFEKDMVVNHARLNRPIRLSYPQKLFAQERISIDEAYPGDVIGLNNPGIFTIGDTIYTGPKLRYAGIPSFSPELFAY
LKNPNPSKFKQFQKGVAELREEGAVQIMYSIDDSKRDPIVAAVGQLQFEVLQYRLESEYNVETRLEMLPYTLARWVENGW
KALENIPSLFNTLVVKDIQDRPVLLFKNEWTLQQFAASNPKIKLSKIAPISDLGPELGDGQ
>Mature_541_residues
MKELDLAKAVEQRRTFAIISHPDAGKTTLTEKLLLYGGAIQEAGAVKARRAQKRATSDWMELEKQRGISITSTVLQFDYR
NIRLNLLDTPGHQDFSEDTYRTLAAADNAVMLEDAGKGLETQTRKLFEVCQMRGLPIFTFINKMDRPSRDPLELMDEIET
ELGLKPYPVNWPIGSGERFTALFDRRTQEFHFFERVSGGRLRVQDTVIKLGDPQIEELIDKDLYYQFKEELELLDGLMPT
LDLKQVHSGKQTPVFFGSAMTNFGVELFLNAFLEYSLPPMPHESTAGLIPPEHPEFTGFVFKLQANMDPRHRDRVAFVRI
CSGKFEKDMVVNHARLNRPIRLSYPQKLFAQERISIDEAYPGDVIGLNNPGIFTIGDTIYTGPKLRYAGIPSFSPELFAY
LKNPNPSKFKQFQKGVAELREEGAVQIMYSIDDSKRDPIVAAVGQLQFEVLQYRLESEYNVETRLEMLPYTLARWVENGW
KALENIPSLFNTLVVKDIQDRPVLLFKNEWTLQQFAASNPKIKLSKIAPISDLGPELGDGQ

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=486, Percent_Identity=27.9835390946502, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI19923640, Length=546, Percent_Identity=24.9084249084249, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=27.4809160305344, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI25306287, Length=546, Percent_Identity=23.992673992674, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI157426893, Length=163, Percent_Identity=31.9018404907975, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI94966754, Length=149, Percent_Identity=30.2013422818792, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1790835, Length=468, Percent_Identity=49.5726495726496, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1789738, Length=500, Percent_Identity=27.4, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=36.5384615384615, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=35.5704697986577, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=491, Percent_Identity=26.8839103869654, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=170, Percent_Identity=34.1176470588235, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=149, Percent_Identity=33.5570469798658, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=146, Percent_Identity=30.1369863013699, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=146, Percent_Identity=30.8219178082192, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=27.9503105590062, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=475, Percent_Identity=25.2631578947368, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322359, Length=510, Percent_Identity=25.6862745098039, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=155, Percent_Identity=33.5483870967742, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=30.5732484076433, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=30.5732484076433, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=28.5123966942149, Blast_Score=178, Evalue=9e-45,
Organism=Drosophila melanogaster, GI221458488, Length=500, Percent_Identity=25.6, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=33.9080459770115, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28574573, Length=156, Percent_Identity=30.1282051282051, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 61636; Mature: 61636

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELDLAKAVEQRRTFAIISHPDAGKTTLTEKLLLYGGAIQEAGAVKARRAQKRATSDWM
CCCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELEKQRGISITSTVLQFDYRNIRLNLLDTPGHQDFSEDTYRTLAAADNAVMLEDAGKGLE
HHHHHCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCH
TQTRKLFEVCQMRGLPIFTFINKMDRPSRDPLELMDEIETELGLKPYPVNWPIGSGERFT
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
ALFDRRTQEFHFFERVSGGRLRVQDTVIKLGDPQIEELIDKDLYYQFKEELELLDGLMPT
HHHHCCCHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LDLKQVHSGKQTPVFFGSAMTNFGVELFLNAFLEYSLPPMPHESTAGLIPPEHPEFTGFV
CCHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
FKLQANMDPRHRDRVAFVRICSGKFEKDMVVNHARLNRPIRLSYPQKLFAQERISIDEAY
EEEECCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCC
PGDVIGLNNPGIFTIGDTIYTGPKLRYAGIPSFSPELFAYLKNPNPSKFKQFQKGVAELR
CCCEEECCCCCEEEECCEEECCCCEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHH
EEGAVQIMYSIDDSKRDPIVAAVGQLQFEVLQYRLESEYNVETRLEMLPYTLARWVENGW
HCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KALENIPSLFNTLVVKDIQDRPVLLFKNEWTLQQFAASNPKIKLSKIAPISDLGPELGDG
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCEEEEECCCHHHCCCCCCCC
Q
C
>Mature Secondary Structure
MKELDLAKAVEQRRTFAIISHPDAGKTTLTEKLLLYGGAIQEAGAVKARRAQKRATSDWM
CCCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELEKQRGISITSTVLQFDYRNIRLNLLDTPGHQDFSEDTYRTLAAADNAVMLEDAGKGLE
HHHHHCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCH
TQTRKLFEVCQMRGLPIFTFINKMDRPSRDPLELMDEIETELGLKPYPVNWPIGSGERFT
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
ALFDRRTQEFHFFERVSGGRLRVQDTVIKLGDPQIEELIDKDLYYQFKEELELLDGLMPT
HHHHCCCHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LDLKQVHSGKQTPVFFGSAMTNFGVELFLNAFLEYSLPPMPHESTAGLIPPEHPEFTGFV
CCHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
FKLQANMDPRHRDRVAFVRICSGKFEKDMVVNHARLNRPIRLSYPQKLFAQERISIDEAY
EEEECCCCCCHHHHEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCC
PGDVIGLNNPGIFTIGDTIYTGPKLRYAGIPSFSPELFAYLKNPNPSKFKQFQKGVAELR
CCCEEECCCCCEEEECCEEECCCCEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHH
EEGAVQIMYSIDDSKRDPIVAAVGQLQFEVLQYRLESEYNVETRLEMLPYTLARWVENGW
HCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KALENIPSLFNTLVVKDIQDRPVLLFKNEWTLQQFAASNPKIKLSKIAPISDLGPELGDG
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCEEEEECCCHHHCCCCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA