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The map label for this gene is aroK

Identifier: 86609195

GI number: 86609195

Start: 1818978

End: 1819541

Strand: Reverse

Name: aroK

Synonym: CYB_1737

Alternate gene names: 86609195

Gene position: 1819541-1818978 (Counterclockwise)

Preceding gene: 86609196

Following gene: 86609192

Centisome position: 59.72

GC content: 61.17

Gene sequence:

>564_bases
ATGATCCACCCCGAGGAAGTTGAGCGCCTGCGCGGCGTAAATGTTTATCTCATTGGGATGATGGGCAGCGGCAAATCCAC
CGTGGGATCGGAGCTGGCAGCCCAGTTGCGCTTTCAATTTTTCGACACCGATGGTCTGGTGGAGCAGGTGGGGGGTTCTT
CCATCGCTCAGATCTTCGCCGAGCATGGGGAAGCCTACTTCCGGGATCTGGAGACGCAGGTGTTGGCCCAGTTGAGCAGC
TACACCCGGCTGGTGATCGCCACAGGCGGCGGCATTGTGTTGCGCCCCAAAAATTGGAGCTACTTGCACCATGGCCTCAC
CGTTTGGCTGGATGTGGCCCCCGAGCTGATTTGGCAGCGGCTCAAGCGGGATCCCGGCCAACGCCCCCTGCTGCAAACCC
CCGACCCCCAAGCTGCCCTGTACAAGCTGATGCAAGAGCGAGAGCCGTTCTACGCCCAAGCCGATGTGCGGGTGCCCATC
CGGTCAGAAACTAGCCCGCCCCAGTTGGTTGAACAGATCTGGCAAGCCATTCGCGCCCGCCTGCGAGACGGGATCCCTTC
CTAG

Upstream 100 bases:

>100_bases
TGGGCAAGTATGTGGAAAAATTGCTGGGCTTGCTGCCCCCCGAGAGCAGCGTTTCTCTGGAGTTTCTGGCCGAGCATGGC
TATATCTAGGTACCTTTCCC

Downstream 100 bases:

>100_bases
CCACCTCGCGCCCAACACAAAAAGGGATCCCATGAGGGATCCCTGAACTCTTCAATACTCAAGTGGATTACTTAAGATTC
GCCGGGAGCGGATCCCTGAC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIHPEEVERLRGVNVYLIGMMGSGKSTVGSELAAQLRFQFFDTDGLVEQVGGSSIAQIFAEHGEAYFRDLETQVLAQLSS
YTRLVIATGGGIVLRPKNWSYLHHGLTVWLDVAPELIWQRLKRDPGQRPLLQTPDPQAALYKLMQEREPFYAQADVRVPI
RSETSPPQLVEQIWQAIRARLRDGIPS

Sequences:

>Translated_187_residues
MIHPEEVERLRGVNVYLIGMMGSGKSTVGSELAAQLRFQFFDTDGLVEQVGGSSIAQIFAEHGEAYFRDLETQVLAQLSS
YTRLVIATGGGIVLRPKNWSYLHHGLTVWLDVAPELIWQRLKRDPGQRPLLQTPDPQAALYKLMQEREPFYAQADVRVPI
RSETSPPQLVEQIWQAIRARLRDGIPS
>Mature_187_residues
MIHPEEVERLRGVNVYLIGMMGSGKSTVGSELAAQLRFQFFDTDGLVEQVGGSSIAQIFAEHGEAYFRDLETQVLAQLSS
YTRLVIATGGGIVLRPKNWSYLHHGLTVWLDVAPELIWQRLKRDPGQRPLLQTPDPQAALYKLMQEREPFYAQADVRVPI
RSETSPPQLVEQIWQAIRARLRDGIPS

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=161, Percent_Identity=38.5093167701863, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1786587, Length=166, Percent_Identity=30.7228915662651, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_SYNJB (Q2JKT7)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477957.1
- ProteinModelPortal:   Q2JKT7
- SMR:   Q2JKT7
- STRING:   Q2JKT7
- GeneID:   3901799
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1737
- TIGR:   CYB_1737
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   Q2JKT7
- ProtClustDB:   PRK00131
- BioCyc:   SSP321332:CYB_1737-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21095; Mature: 21095

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 44-44 BINDING 68-68 BINDING 90-90 BINDING 128-128 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHPEEVERLRGVNVYLIGMMGSGKSTVGSELAAQLRFQFFDTDGLVEQVGGSSIAQIFA
CCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHH
EHGEAYFRDLETQVLAQLSSYTRLVIATGGGIVLRPKNWSYLHHGLTVWLDVAPELIWQR
HHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LKRDPGQRPLLQTPDPQAALYKLMQEREPFYAQADVRVPIRSETSPPQLVEQIWQAIRAR
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHH
LRDGIPS
HHCCCCC
>Mature Secondary Structure
MIHPEEVERLRGVNVYLIGMMGSGKSTVGSELAAQLRFQFFDTDGLVEQVGGSSIAQIFA
CCCHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHH
EHGEAYFRDLETQVLAQLSSYTRLVIATGGGIVLRPKNWSYLHHGLTVWLDVAPELIWQR
HHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LKRDPGQRPLLQTPDPQAALYKLMQEREPFYAQADVRVPIRSETSPPQLVEQIWQAIRAR
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHH
LRDGIPS
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA